LOCUS       sequence-243182-       11621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CD G124D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CD-G124D
FEATURES             Location/Qualifiers
     source          1..11621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6581..6605)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6613..6631
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6656..6675)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6657..6675)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6681..6701)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6755..6796
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6817..7384
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6834..7386
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6978..6995)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7205..7224
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7398..7997
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7398..7417)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7894..7914
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8011..8599
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8064..8084)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8482..8493)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8674..8907
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9026..9044)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9112..9216
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9217..9285,9286..10077)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9435..9454)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10248..10836
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10737..10756
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10990..11007
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11247..11268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11283..11288,11289..11306,11307..11313)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11321..11337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11326..11348
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11345..11361
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11345..11361
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11411..11430
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcaccatg ccccctgggg tggactgccc catggaattc
     3481 tggaccaagg aggagaatca gagcgttgtg gttgacttcc tgctgcccac aggggtctac
     3541 ctgaacttcc ctgtgtcccg caatgccaac ctcagcacca tcaagcagct gctgtggcac
     3601 cgcgcccagt atgagccgct cttccacatg ctcagtggcc ccgaggccta tgtgttcacc
     3661 tgcatcaacc agacagcgga gcagcaagag ctggaggacg agcaacggcg tctgtgtgac
     3721 gtgcagccct tcctgcccgt cctgcgcctg gtggcccgtg agggcgaccg cgtgaagaag
     3781 ctcatcaact cacagatcag cctcctcatc ggcaaagacc tccacgagtt tgactccttg
     3841 tgcgacccag aagtgaacga ctttcgcgcc aagatgtgcc aattctgcga ggaggcggcc
     3901 gcccgccggc agcagctggg ctgggaggcc tggctgcagt acagtttccc cctgcagctg
     3961 gagccctcgg ctcaaacctg ggggcctggt accctgcggc tcccgaaccg ggcccttctg
     4021 gtcaacgtta agtttgaggg cagcgaggag agcttcacct tccaggtgtc caccaaggac
     4081 gtgccgctgg cgctgatggc ctgtgccctg cggaagaagg ccacagtgtt ccggcagccg
     4141 ctggtggagc agccggaaga ctacacgctg caggtgaacg gcaggcatga gtacctgtat
     4201 ggcagctacc cgctctgcca gttccagtac atctgcagct gcctgcacag tgggttgacc
     4261 cctcacctga ccatggtcca ttcctcctcc atcctcgcca tgcgggatga gcagagcaac
     4321 cctgcccccc aggtccagaa accgcgtgcc aaaccacctc ccattcctgc gaagaagcct
     4381 tcctctgtgt ccctgtggtc cctggagcag ccgttccgca tcgagctcat ccagggcagc
     4441 aaagtgaacg ccgacgagcg gatgaagctg gtggtgcagg ccgggctttt ccacggcaac
     4501 gagatgctgt gcaagacggt gtccagctcg gaggtgagcg tgtgctcgga gcccgtgtgg
     4561 aagcagcggc tggagttcga catcaacatc tgcgacctgc cccgcatggc ccgtctctgc
     4621 tttgcgctgt acgccgtgat cgagaaagcc aagaaggctc gctccaccaa gaagaagtcc
     4681 aagaaggcgg actgccccat tgcctgggcc aacctcatgc tgtttgacta caaggaccag
     4741 cttaagaccg gggaacgctg cctctacatg tggccctccg tcccagatga gaagggcgag
     4801 ctgctgaacc ccacgggcac tgtgcgcagt aaccccaaca cggatagcgc cgctgccctg
     4861 ctcatctgcc tgcccgaggt ggccccgcac cccgtgtact accccgccct ggagaagatc
     4921 ttggagctgg ggcgacacag cgagtgtgtg catgtcaccg aggaggagca gctgcagctg
     4981 cgggaaatcc tggagcggcg ggggtctggg gagctgtatg agcacgagaa ggacctggtg
     5041 tggaagctgc ggcatgaagt ccaggagcac ttcccggagg cgctagcccg gctgctgctg
     5101 gtcaccaagt ggaacaagca tgaggatgtg gcccagatgc tctacctgct gtgctcctgg
     5161 ccggagctgc ccgtcctgag cgccctggag ctgctagact tcagcttccc cgattgccac
     5221 gtaggctcct tcgccatcaa gtcgctgcgg aaactgacgg acgatgagct gttccagtac
     5281 ctgctgcagc tggtgcaggt gctcaagtac gagtcctacc tggactgcga gctgaccaaa
     5341 ttcctgctgg accgggccct ggccaaccgc aagatcggcc acttcctttt ctggcacctc
     5401 cgctccgaga tgcacgtgcc gtcggtggcc ctgcgcttcg gcctcatcct ggaggcctac
     5461 tgcaggggca gcacccacca catgaaggtg ctgatgaagc agggggaagc actgagcaaa
     5521 ctgaaggccc tgaatgactt cgtcaagctg agctctcaga agacccccaa gccccagacc
     5581 aaggagctga tgcacttgtg catgcggcag gaggcctacc tagaggccct ctcccacctg
     5641 cagtccccac tcgaccccag caccctgctg gctgaagtct gcgtggagca gtgcaccttc
     5701 atggactcca agatgaagcc cctgtggatc atgtacagca acgaggaggc aggcagcggc
     5761 ggcagcgtgg gcatcatctt taagaacggg gatgacctcc ggcaggacat gctgaccctg
     5821 cagatgatcc agctcatgga cgtcctgtgg aagcaggagg ggctggacct gaggatgacc
     5881 ccctatggct gcctccccac cggggaccgc acaggcctca ttgaggtggt actccgttca
     5941 gacaccatcg ccaacatcca actcaacaag agcaacatgg cagccacagc cgccttcaac
     6001 aaggatgccc tgctcaactg gctgaagtcc aagaacccgg gggaggccct ggatcgagcc
     6061 attgaggagt tcaccctctc ctgtgctggc tattgtgtgg ccacatatgt gctgggcatt
     6121 ggcgatcggc acagcgacaa catcatgatc cgagagagtg ggcagctgtt ccacattgat
     6181 tttggccact ttctggggaa tttcaagacc aagtttggaa tcaaccgcga gcgtgtccca
     6241 ttcatcctca cctacgactt tgtccatgtg attcagcagg ggaagactaa taatagtgag
     6301 aaatttgaac ggttccgggg ctactgtgaa agggcctaca ccatcctgcg gcgccacggg
     6361 cttctcttcc tccacctctt tgccctgatg cgggcggcag gcctgcctga gctcagctgc
     6421 tccaaagaca tccagtatct caaggactcc ctggcactgg ggaaaacaga ggaggaggca
     6481 ctgaagcact tccgagtgaa gtttaacgaa gccctccgtg agagctggaa aaccaaagtg
     6541 aactggctgg cccacaacgt gtccaaagac aacaggcagt tgccaacttt cttgtacaaa
     6601 gtggtgatat ccaattaacc ctcactaaag gaaagaatta gtatgataga tgagacccta
     6661 tagtgagtcg tattaccacc caacttttct atacaaagtg gttgatatcc agcacagtgg
     6721 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     6781 cggtctcgat tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac
     6841 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     6901 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6961 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     7021 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     7081 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     7141 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     7201 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     7261 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     7321 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     7381 ataatatggc cacacatatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     7441 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     7501 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     7561 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     7621 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     7681 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     7741 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     7801 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     7861 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7921 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7981 gcaagcccgg tgcctaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa
     8041 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8101 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8161 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8221 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8281 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8341 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8401 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8461 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8521 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8581 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag
     8641 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     8701 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8761 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8821 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8881 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     8941 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg
     9001 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     9061 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     9121 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9181 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9241 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9301 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9361 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9421 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     9481 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9541 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9601 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9661 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9721 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9781 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9841 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9901 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9961 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10021 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10081 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10141 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10201 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10261 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10321 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10381 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10441 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10501 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10561 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10621 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10681 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10741 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10801 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10861 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10921 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10981 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    11041 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta
    11101 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    11161 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg
    11221 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    11281 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    11341 cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg
    11401 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    11461 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    11521 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    11581 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
