LOCUS       sequence-243195-       11693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA E110delE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-E110delE
FEATURES             Location/Qualifiers
     source          1..11693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6653..6677)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6685..6703
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6728..6747)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6729..6747)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6753..6773)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6827..6868
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6889..7456
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6906..7458
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7050..7067)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7277..7296
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7470..8069
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7470..7489)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7966..7986
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8083..8671
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8136..8156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8554..8565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8746..8979
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9098..9116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9184..9288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9289..9357,9358..10149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9507..9526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10320..10908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10809..10828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11062..11079
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11319..11340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11355..11360,11361..11378,11379..11385)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11393..11409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11398..11420
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11417..11433
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11417..11433
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11483..11502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg
     3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata
     3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa
     3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta
     3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac
     3721 cttcggcttt ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgaaaagatc
     3781 ctcaatcgag aaattggttt tgctatcggc atgccagtgt gtgaatttga tatggttaaa
     3841 gatccagaag tacaggactt ccgaagaaat attctgaacg tttgtaaaga agctgtggat
     3901 cttagggacc tcaattcacc tcatagtaga gcaatgtatg tctatcctcc aaatgtagaa
     3961 tcttcaccag aattgccaaa gcacatatat aataaattag ataaagggca aataatagtg
     4021 gtgatctggg taatagtttc tccaaataat gacaagcaga agtatactct gaaaatcaac
     4081 catgactgtg taccagaaca agtaattgct gaagcaatca ggaaaaaaac tcgaagtatg
     4141 ttgctatcct ctgaacaact aaaactctgt gttttagaat atcagggcaa gtatatttta
     4201 aaagtgtgtg gatgtgatga atacttccta gaaaaatatc ctctgagtca gtataagtat
     4261 ataagaagct gtataatgct tgggaggatg cccaatttga tgttgatggc taaagaaagc
     4321 ctttattctc aactgccaat ggactgtttt acaatgccat cttattccag acgcatttcc
     4381 acagctacac catatatgaa tggagaaaca tctacaaaat ccctttgggt tataaatagt
     4441 gcactcagaa taaaaattct ttgtgcaacc tacgtgaatg taaatattcg agacattgat
     4501 aagatctatg ttcgaacagg tatctaccat ggaggagaac ccttatgtga caatgtgaac
     4561 actcaaagag taccttgttc caatcccagg tggaatgaat ggctgaatta tgatatatac
     4621 attcctgatc ttcctcgtgc tgctcgactt tgcctttcca tttgctctgt taaaggccga
     4681 aagggtgcta aagaggaaca ctgtccattg gcatggggaa atataaactt gtttgattac
     4741 acagacactc tagtatctgg aaaaatggct ttgaatcttt ggccagtacc tcatggatta
     4801 gaagatttgc tgaaccctat tggtgttact ggatcaaatc caaataaaga aactccatgc
     4861 ttagagttgg agtttgactg gttcagcagt gtggtaaagt tcccagatat gtcagtgatt
     4921 gaagagcatg ccaattggtc tgtatcccga gaagcaggat ttagctattc ccacgcagga
     4981 ctgagtaaca gactagctag agacaatgaa ttaagggaaa atgacaaaga acagctcaaa
     5041 gcaatttcta cacgagatcc tctctctgaa atcactgagc aggagaaaga ttttctatgg
     5101 agtcacagac actattgtgt aactatcccc gaaattctac ccaaattgct tctgtctgtt
     5161 aaatggaatt ctagagatga agtagcccag atgtattgct tggtaaaaga ttggcctcca
     5221 atcaaacctg aacaggctat ggaacttctg gactgtaatt acccagatcc tatggttcga
     5281 ggttttgctg ttcggtgctt ggaaaaatat ttaacagatg acaaactttc tcagtattta
     5341 attcagctag tacaggtcct aaaatatgaa caatatttgg ataacttgct tgtgagattt
     5401 ttactgaaga aagcattgac taatcaaagg attgggcact ttttcttttg gcatttaaaa
     5461 tctgagatgc acaataaaac agttagccag aggtttggcc tgcttttgga gtcctattgt
     5521 cgtgcatgtg ggatgtattt gaagcacctg aataggcaag tcgaggcaat ggaaaagctc
     5581 attaacttaa ctgacattct caaacaggag aagaaggatg aaacacaaaa ggtacagatg
     5641 aagtttttag ttgagcaaat gaggcgacca gatttcatgg atgctctaca gggctttctg
     5701 tctcctctaa accctgctca tcaactagga aacctcaggc ttgaagagtg tcgaattatg
     5761 tcctctgcaa aaaggccact gtggttgaat tgggagaacc cagacatcat gtcagagtta
     5821 ctgtttcaga acaatgagat catctttaaa aatggggatg atttacggca agatatgcta
     5881 acacttcaaa ttattcgtat tatggaaaat atctggcaaa atcaaggtct tgatcttcga
     5941 atgttacctt atggttgtct gtcaatcggt gactgtgtgg gacttattga ggtggtgcga
     6001 aattctcaca ctattatgca aattcagtgc aaaggcggct tgaaaggtgc actgcagttc
     6061 aacagccaca cactacatca gtggctcaaa gacaagaaca aaggagaaat atatgatgca
     6121 gccattgacc tgtttacacg ttcatgtgct ggatactgtg tagctacctt cattttggga
     6181 attggagatc gtcacaatag taacatcatg gtgaaagacg atggacaact gtttcatata
     6241 gattttggac actttttgga tcacaagaag aaaaaatttg gttataaacg agaacgtgtg
     6301 ccatttgttt tgacacagga tttcttaata gtgattagta aaggagccca agaatgcaca
     6361 aagacaagag aatttgagag gtttcaggag atgtgttaca aggcttatct agctattcga
     6421 cagcatgcca atctcttcat aaatcttttc tcaatgatgc ttggctctgg aatgccagaa
     6481 ctacaatctt ttgatgacat tgcatacatt cgaaagaccc tagccttaga taaaactgag
     6541 caagaggctt tggagtattt catgaaacaa atgaatgatg cacatcatgg tggctggaca
     6601 acaaaaatgg attggatctt ccacacaatt aaacagcatg cattgaacta gaacccagct
     6661 ttcttgtaca aagtggtgat atccaattaa ccctcactaa aggaagatga tagttaagtg
     6721 taagttaccc tatagtgagt cgtattacca cccaactttt ctatacaaag tggttgatat
     6781 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
     6841 taaccctctc ctcggtctcg attctacgcg taccggttag taatgagatc cctccccccc
     6901 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     6961 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     7021 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     7081 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     7141 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     7201 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     7261 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     7321 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     7381 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     7441 aaaaacacga tgataatatg gccacacata tgaccgagta caagcccacg gtgcgcctcg
     7501 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     7561 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     7621 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     7681 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     7741 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     7801 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     7861 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     7921 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     7981 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     8041 ggtgcatgac ccgcaagccc ggtgcctaaa tcgatagatc ctaatcaacc tctggattac
     8101 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8161 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8221 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8281 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8341 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8401 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8461 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8521 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8581 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8641 agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca
     8701 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8761 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8821 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8881 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8941 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     9001 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa
     9061 ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     9121 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     9181 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     9541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9901 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9961 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    10021 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    10081 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    10141 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    10201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10441 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    11041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    11101 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac
    11161 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    11221 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg
    11281 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    11341 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    11401 ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca
    11461 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    11521 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    11581 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    11641 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
