LOCUS       sequence-243198-        5087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of immunoglobulin light chains in mammalian cells, mouse 
            (C57BL/6), lambda 2 constant region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Lorenzo C, Delgado P, Busse CE, Sanz-Bravo A, Martos-Folgado I, 
            Bonzon-Kulichenko E, Ferrarini A, Gonzalez-Valdes IB, Mur SM, 
            Roldan-Montero R, Martinez-Lopez D, Martin-Ventura JL, Vazquez J, 
            Wardemann H, Ramiro AR
  TITLE     ALDH4A1 is an atherosclerosis auto-antigen targeted by protective 
            antibodies.
  JOURNAL   Nature. 2021 Jan;589(7841):287-292. doi: 10.1038/s41586-020-2993-2. 
            Epub 2020 Dec 2.
  PUBMED    33268892
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2993-2"; journalName: "Nature"; date: "2021-01";
            volume: "589"; issue: "7841"; pages: "287-292"
COMMENT     Sequence Label: AbVec2.1-mIglc2
FEATURES             Location/Qualifiers
     source          1..5087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        249..628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        629..832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     783..803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     829..853
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1063..1081
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1063..1080
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1094..1113
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1622..1631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1635..1769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1672..1691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1726..1745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        1838..2195
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1838..2029
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1861..1881)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2046..2181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2215..2232)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2215..2231)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2224..2246)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2444..2899
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2531..2550)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2741..2762
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2916..2935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3035..3057
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3095..3113)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3181..3285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3286..3354,3355..4146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3504..3523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4317..4905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4806..4825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5059..5076
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccaactggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attaattcga
      241 gctcgcccga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      841 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      901 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
      961 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
     1021 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
     1081 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
     1141 ggttctatcg attgaattcc accatgggat ggtcatgtat catccttttt ctagtagcaa
     1201 ctgcaaccgg ttgactaact agctggccag cccaagagca ctcccactct caccgtgttt
     1261 ccaccttcct ctgaggagct caaggaaaac aaagccacac tggtgtgtct gatttccaac
     1321 ttttccccga gtggtgtgac agtggcctgg aaggcaaatg gtacacctat cacccagggt
     1381 gtggacactt caaatcccac caaagagggc aacaagttca tggcctccag cttcctacat
     1441 ttgacatcgg accagtggag atctcacaac agttttacct gtcaagttac acatgaaggg
     1501 gacactgtgg agaagagtct gtctcctgca gaatgtctct aagaacccag gtttctcctt
     1561 agcctgggaa ccctgcagct tttagagacc cagggtgggg tctcttcttt atataagctt
     1621 ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1681 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1741 tcatcaatgt atcttatcat gtctggatcg ggaattaatt cggcgcagca ccatggcctg
     1801 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccatctgt
     1861 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     1921 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     1981 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     2041 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     2101 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     2161 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct gttaacagct tggcactggc
     2221 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2281 agcacatccc cctttcgcca gttggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2341 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     2401 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     2461 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2521 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2581 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2641 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2701 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2761 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     2821 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     2881 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     2941 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     3001 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     3061 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     3121 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     3181 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3241 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3301 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3361 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3421 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3481 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3541 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3601 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3661 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3721 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3781 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3841 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3901 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3961 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4021 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4081 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4141 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4201 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4261 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4321 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4381 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4441 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     4501 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4561 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4621 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4681 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4741 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4801 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4861 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4921 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4981 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5041 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaga
//
