LOCUS       sequence-243218-       11627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescently tagged p65 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity 
            AJ, Zhang F, Blainey PC
  TITLE     Optical Pooled Screens in Human Cells.
  JOURNAL   Cell. 2019 Oct 17;179(3):787-799.e17. doi: 
            10.1016/j.cell.2019.09.016.
  PUBMED    31626775
REFERENCE   2  (bases 1 to 11627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.09"; journalName: "Cell"; date: "2019-10-17- 
            17"; volume: "179"; issue: "3"; pages: "787-799.e17"
COMMENT     Sequence Label: p65-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..11627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2687..3864
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2918..3855
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3812..3832
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3871..3880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5158..5529
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             5554..6255
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEEL
                     NLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGA
                     SLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTF
                     KWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKA
                     FTDVMGMDELYK"
     regulatory      5803..5812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6265..6291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6319..6907
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6372..6392)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6790..6801)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6910..6926)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6911..6927)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7432..7612
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7638..7655)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7644..7868
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7914..8342
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8001..8020)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8211..8232
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8351..8371)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8356..8685
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8536..8671
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8598..8617
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8733..8780
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8799..9173
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9303..9424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9340..9359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9394..9413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9473..9489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9473..9489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9486..9508)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9497..9513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9521..9527,9528..9545,9546..9551))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9566..9587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9704..9721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9875..10463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9955..9974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10634..11425,11426..11494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11257..11276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11495..11599)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccactggctg
     3001 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     3061 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     3121 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     3181 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     3241 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     3301 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     3361 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     3421 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     3481 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     3541 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     3601 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     3661 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     3721 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     3781 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     3841 tttcttccat ttcaggtgtc gtgatgtaca gccaccatgg acgaactgtt ccccctcatc
     3901 ttcccggcag agccagccca ggcctctggc ccctatgtgg agatcattga gcagcccaag
     3961 cagcggggca tgcgcttccg ctacaagtgc gaggggcgct ccgcgggcag catcccaggc
     4021 gagaggagca cagataccac caagacccac cccaccatca agatcaatgg ctacacagga
     4081 ccagggacag tgcgcatctc cctggtcacc aaggaccctc ctcaccggcc tcacccccac
     4141 gagcttgtag gaaaggactg ccgggatggc ttctatgagg ctgagctctg cccggaccgc
     4201 tgcatccaca gtttccagaa cctgggaatc cagtgtgtga agaagcggga cctggagcag
     4261 gctatcagtc agcgcatcca gaccaacaac aaccccttcc aagttcctat agaagagcag
     4321 cgtggggact acgacctgaa tgctgtgcgg ctctgcttcc aggtgacagt gcgggaccca
     4381 tcaggcaggc ccctccgcct gccgcctgtc ctttctcatc ccatctttga caatcgtgcc
     4441 cccaacactg ccgagctcaa gatctgccga gtgaaccgaa actctggcag ctgcctcggt
     4501 ggggatgaga tcttcctact gtgtgacaag gtgcagaaag aggacattga ggtgtatttc
     4561 acgggaccag gctgggaggc ccgaggctcc ttttcgcaag ctgatgtgca ccgacaagtg
     4621 gccattgtgt tccggacccc tccctacgca gaccccagcc tgcaggctcc tgtgcgtgtc
     4681 tccatgcagc tgcggcggcc ttccgaccgg gagctcagtg agcccatgga attccagtac
     4741 ctgccagata cagacgatcg tcaccggatt gaggagaaac gtaaaaggac atatgagacc
     4801 ttcaagagca tcatgaagaa gagtcctttc agcggaccca ccgacccccg gcctccacct
     4861 cgacgcattg ctgtgccttc ccgcagctca gcttctgtcc ccaagccagc accccagccc
     4921 tatcccttta cgtcatccct gagcaccatc aactatgatg agtttcccac catggtgttt
     4981 ccttctgggc agatcagcca ggcctcggcc ttggccccgg cccctcccca agtcctgccc
     5041 caggctccag cccctgcccc tgctccagcc atggtatcag ctctggccca ggccccagcc
     5101 cctgtcccag tcctagcccc aggccctcct caggctgtgg ccccacctgc ccccaagccc
     5161 acccaggctg gggaaggaac gctgtcagag gccctgctgc agctgcagtt tgatgatgaa
     5221 gacctggggg ccttgcttgg caacagcaca gacccagctg tgttcacaga cctggcatcc
     5281 gtcgacaact ccgagtttca gcagctgctg aaccagggca tacctgtggc cccccacaca
     5341 actgagccca tgctgatgga gtaccctgag gctataactc gcctagtgac aggggcccag
     5401 aggccccccg acccagctcc tgctccactg ggggccccgg ggctccccaa tggcctcctt
     5461 tcaggagatg aagacttctc ctccattgcg gacatggact tctcagccct gctgagtcag
     5521 atcagctccg gaggcggctc cggtggagga tccgtgagca agggcgagga ggataacgcc
     5581 tctctcccag cgacacatga gttacacatc tttggctcca tcaacggtgt ggactttgac
     5641 atggtgggtc agggcaccgg caatccaaat gatggttatg aggagttaaa cctgaagtcc
     5701 accaagggtg acctccagtt ctccccctgg attctggtcc ctcatatcgg gtatggcttc
     5761 catcagtacc tgccctaccc tgacgggatg tcgcctttcc aggccgccat ggtagatggc
     5821 tccggatacc aagtccatcg cacaatgcag tttgaagatg gtgcctccct tactgttaac
     5881 taccgctaca cctacgaggg aagccacatc aaaggagagg cccaggtgaa ggggactggt
     5941 ttccctgctg acggtcctgt gatgaccaac tcgctgaccg ctgcggactg gtgcaggtcg
     6001 aagaaaactt accccaacga caaaaccatc atcagtacct ttaagtggag ttacaccact
     6061 ggaaatggca agcgctaccg gagcactgcg cggaccacct acacctttgc caagccaatg
     6121 gcggctaact atctgaagaa ccagccgatg tacgtgttcc gtaagacgga gctcaagcac
     6181 tccaagaccg agctcaactt caaggagtgg caaaaggcct ttaccgatgt gatgggcatg
     6241 gacgagctgt ataagggaag atcctaccca tacgatgttc cagattacgc ttgagaattc
     6301 gatatcaagc ttatcggtaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6361 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6421 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6481 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6541 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6601 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6661 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6721 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6781 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6841 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6901 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     6961 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     7021 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     7081 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7141 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     7201 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     7261 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     7321 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     7381 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     7441 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7501 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7561 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     7621 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7681 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     7741 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     7801 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     7861 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     7921 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7981 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8041 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8101 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8161 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8221 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8281 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8341 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     8401 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8461 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     8521 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8581 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     8641 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     8701 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     8761 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     8821 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     8881 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     8941 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     9001 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     9061 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     9121 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     9181 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     9241 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     9301 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9421 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     9481 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9541 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9601 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9661 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9721 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9781 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9841 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9901 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9961 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10021 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10081 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10141 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10201 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10261 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    10321 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10381 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    10441 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    10501 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    10561 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    10621 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    10681 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10741 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10801 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10861 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10921 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10981 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    11041 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    11101 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    11161 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    11221 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    11281 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    11341 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    11401 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    11461 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    11521 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    11581 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
