LOCUS       sequence-243219-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry plasmid for guide cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity 
            AJ, Zhang F, Blainey PC
  TITLE     Optical Pooled Screens in Human Cells.
  JOURNAL   Cell. 2019 Oct 17;179(3):787-799.e17. doi: 
            10.1016/j.cell.2019.09.016.
  PUBMED    31626775
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.09"; journalName: "Cell"; date: "2019-10-17- 
            17"; volume: "179"; issue: "3"; pages: "787-799.e17"
COMMENT     Sequence Label: CROPseq-Guide-Zeo
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2180..2255
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     LTR             2308..2488
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2566..2687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2603..2622)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2657..2676
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2727..2862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2882..2901)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2883..2901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2911..2928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2911..2927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2920..2942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3069..3524
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3156..3175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3366..3387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3550..3654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3655..3723,3724..4515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3873..3892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4686..5274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5175..5194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5428..5445
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5562..5583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5598..5603,5604..5621,5622..5628)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5636..5652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5641..5663
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5660..5676
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5660..5676
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5695..5715
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5697..5715
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5743..5969
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5970..6150
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6197..6322
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6815..7048
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7233..7277
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7458..7575
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7714..8892
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7945..8883
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8840..8860
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8899..8908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8905..9279
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    9295..9883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9348..9368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9766..9777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      301 agacggttgt aaatgagcac acaaaataca catgctaaaa tattatattc tatgaccttt
      361 ataaaatcaa ccaaaatctt ctttttaata actttagtat caataattag aatttttatg
      421 ttcctttttg caaactttta ataaaaatga gcaaaataaa aaaacgctag ttttagtaac
      481 tcgcgttgtt ttcttcacct ttaataatag ctactccacc acttgttcct aagcggtcag
      541 ctcctgcttc aatcattttt tgagcatctt caaatgttct aactccacca gctgctttaa
      601 ctaaagcatt gtctttaaca actgacttca ttagtttaac atcttcaaat gttgcacctg
      661 attttgaaaa tcctgttgat gttttaacaa attctaatcc agcttcaaca gctatttcac
      721 aagctttcat gatttcttct tttgttaata aacaattttc cataatacat ttaacaacat
      781 gtgatccagc tgcttttttt acagctttca tgtcttctaa aactaattca taatttttgt
      841 cttttaatgc accaatattt aataccatat caatttctgt tgcaccatct ttaattgctt
      901 cagaaacttc gaatgctttt gtagctgttg tgcatgcacc tagaggaaaa cctacaacat
      961 ttgttattcc tacatttgtg ccttttaata attctttaca atagcttgtt caatatgaat
     1021 taacacaaac tgttgcaaaa tcaaattcaa ttgcttcatc acataattgt ttaatttcag
     1081 ctttcgtagc atcttgtttt aataatgtgt gatctatata tttgtttagt ttcatttttt
     1141 ctcctatata ttcattttta attttaattc tttaataatt tcgtctactt taactttagc
     1201 gttttgaaca gattcaccaa cacctataaa ataaattttt agtttaggtt cagttccact
     1261 tgggcgaaca gcaaatcatg acttatcttc taaataaaat tttagtaagt cttgtcctgg
     1321 catattatac attccatcga tgtagtcttc aacattaaca actttaagtc cagcaatttg
     1381 agttaagggt gttgctctca atgatttcat taatggttca atttttaatt tcttttcttc
     1441 tggtttaaaa ttcaagttta aagtgaaagt gtaatatgca cccatttctt taaataaatc
     1501 ttctaaatag tctactaatg ttttattttg ttttttataa aatcaagcag cctctgctat
     1561 taatatagaa gcttgtattc catctttatc tctagctgag tcatcaatta catatccata
     1621 actttcttca taagcaaaaa caaaatttaa tccgttatct tcttctttag caatttctct
     1681 acccattcat ttaaatccag ttaaagtttt tacaatatta actccatatt tttcatgagc
     1741 gattctatca cccaaatcac ttgttacaaa acttgaatat agagccggat tttttggaat
     1801 gctatttaag cgttttagat ttgataattt tcaatcaatt aaaattggtc ctgtttgatt
     1861 tccatctaat cttacaaaat gaccatcatg ttttattgcc attccaaatc tgtcagcatc
     1921 tgggtcattc ataataataa tatctgcatc atgtttaata ccatattcaa gcggtatttt
     1981 tcatgcagga tcaaattctg gatttggatt tacaacattt ttaaatgttt catcttcaaa
     2041 tgcatgctct tcaacctcaa taacgttata tcctgattca cgtaatattt ttggggtaaa
     2101 tttagttcct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2161 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc
     2221 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc gtaactagat
     2281 cttgagacac tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     2341 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     2401 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     2461 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     2521 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     2581 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2641 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     2701 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     2761 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     2821 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     2881 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     2941 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     3001 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     3061 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3121 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3181 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3241 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3301 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3361 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     3421 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3481 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     3541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3901 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3961 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4021 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4081 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4141 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4201 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4261 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4321 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4381 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4441 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4501 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4681 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4741 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4801 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     4861 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4921 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4981 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5041 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5101 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5161 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5221 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5281 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5341 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5401 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5461 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     5521 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     5581 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     5641 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     5701 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     5761 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     5821 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     5881 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     5941 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     6001 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6061 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6121 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     6181 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     6241 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     6301 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     6361 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     6421 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     6481 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     6541 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     6601 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     6661 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     6721 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     6781 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     6841 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     6901 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     6961 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     7021 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     7081 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     7141 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     7201 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     7261 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     7321 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     7381 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagaatctt gagacaaatg
     7441 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     7501 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     7561 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc
     7621 gagggggccc gggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     7681 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     7741 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc
     7801 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     7861 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     7921 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     7981 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     8041 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     8101 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     8161 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     8221 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     8281 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     8341 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     8401 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     8461 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     8521 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     8581 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     8641 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     8701 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     8761 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     8821 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     8881 caggtgtcgt gacgtacggc caccatggcc aagttgacca gtgccgttcc ggtgctcacc
     8941 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     9001 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     9061 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     9121 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     9181 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     9241 ggcaactgcg tgcacttcgt ggccgaggag caggactgaa cgcgttaagt cgacaatcaa
     9301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9421 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     9481 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     9541 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     9601 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     9661 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     9721 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     9781 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9841 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     9901 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9961 ggctaattca ctcccaacga agacaagat
//
