LOCUS       sequence-24322-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     T4 Lysozyme Plasmids - Tray 4
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme R119-[A] WT*
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(716..738)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    727..743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctancgaaaa agcccgctca ttaggcgggc tcagatctgc tcatgtttga cagcttatca
       61 tcgataagct tcggcaataa attcttgata tgttttcata tgtttccttt aaatatttta
      121 attagtaatt gtctattcaa gtaattgtga atatactatc acaattccaa gagaaagtaa
      181 acagctttat agatttttat acgcgtccca agtgccagtt ctaaacgttg taatgactcg
      241 ttttgcgcga ttaggtgttt gattatacca tctactttta gctaagttaa ctgctgcttc
      301 atcccagcgt ttttgttgaa gcattgcacg taaagagtta gtaaatcctg ccacaccggt
      361 ttctcccatt tggaaaacca tattaatcaa tgcagcgcga cgaaccgcat caagagaatc
      421 ataaaccggt tttaatttag catttctcag aattccgcga acagcagcat caacatcctg
      481 attaaagagt ttttcagcct catcttttgt aattacacca ttagtattac gcccaatagc
      541 tttatctaat tcagatttag cagcattaag tgatggactt tttgtaagca aatgaccgat
      601 gccaatagtg taatagcctt ctgtgtcttt atagatttta agtctaagac cttcatctat
      661 acgtaacatt tcaaatatat tcataatacc tcctaagcat cgatggatcc tgtttcctgt
      721 gtgaaattgt tatccgctca caattccaca cattatacga gccgatgata attgtcacag
      781 gggatgggag tagctgacct gttccgggtg aatgtaccgc tacatcncaa taacgagcga
      841 na
//
