LOCUS       sequence-243224-       14409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eukaryotic expression of the fusion protein used for sulfa-drugs 
            sensing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14409)
  AUTHORS   Scarabelli S, Tan KT, Griss R, Hovius R, D'Alessandro PL, Vorherr T,
            Johnsson K
  TITLE     Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins.
  JOURNAL   ACS Sens. 2017 Aug 25;2(8):1191-1197. doi: 
            10.1021/acssensors.7b00331. Epub 2017 Aug 2.
  PUBMED    28766337
REFERENCE   2  (bases 1 to 14409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.7b00331"; journalName: "ACS Sens"; date: 
            "2017-08-25- 25"; volume: "2"; issue: "8"; pages: "1191-1197"
COMMENT     Sequence Label: pEBTet-SNAP-HCA_sensor
FEATURES             Location/Qualifiers
     source          1..14409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..36
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             43..57
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             616..1329
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     CDS             join(1453..1455,1456..1458,1459..1971)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(1498..1519)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1759..1778)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2106..2127
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2962..2991
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(3032..3049)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3038..3149
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3690..5479
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(8143..8161)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8229..8333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8334..8402,8403..9194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8552..8571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9365..9953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9854..9873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(10142..10162)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10160..10457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             10534..11133
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10534..10553)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11030..11050
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    11224..11358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11496..11515)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11792..12053
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          12170..12742
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     12750..12769
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     12797..12816
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12797..12815
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             12826..13446
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(12899..12918)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    13488..13609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        13664..14043
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14044..14247
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     14198..14218
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    14249..14267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    14270..14288
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     14298..14322
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 accatggcaa gctggagcca cccgcagttc gaaaagggtg cagatgacga cgacaaggta
       61 ccaatggaca aggattgtga aatgaaacgc accacactgg acagcccttt ggggaagctg
      121 gagctgtctg gttgtgagca gggtctgcac gaaataattt ttctgggcaa ggggacgtct
      181 gcagctgatg ccgtggaggt cccagccccc gctgcggttc tcggaggtcc ggagcccctg
      241 atgcaggcta cagcctggct gaatgcctat ttccaccagc ccgaggctat cgaagagttc
      301 cccgtgccgg ctcttcacca tcccgttttc cagcaagagt cgttcaccag acaggtgtta
      361 tggaagctgc tgaaggttgt gaaattcgga gaagtgattt cttactctca cttagcagcc
      421 ctggcaggca accccgcagc cacggcagca gtgaagacgg cactgagtgg caatcctgtc
      481 cctatcctga tcccgtgcca cagagtggtc cagggggatt tggatgtggg cggttacgag
      541 ggtggactgg ccgtgaagga atggcttctg gcccatgaag gccaccggtt ggggaagcca
      601 ggcttgggaa ctagtatgtc taaaggtgaa gaattattca ctggtgttgt cccaattttg
      661 gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt
      721 gatgctacgt acggtaaatt gaccttaaaa ttactctgta ctactggtaa attgccagtt
      781 ccatggccaa ccttagtcac tactttaggt tatggtgttc aatgttttgc tagataccca
      841 gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa
      901 agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa
      961 ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac
     1021 attttaggtc acaaattgga atacaactat aactctcaca atgtttacat cactgctgac
     1081 aaacaaaaga atggtatcaa agctaacttc aaaattagac acaacattga agatggtggt
     1141 gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta
     1201 ccagacaacc attacttatc ctatcaatct gccttattca aagatccaaa cgaaaagaga
     1261 gaccacatgg tcttgttaga atttttgact gctgctggta ttaccgaggg tatgaatgaa
     1321 ttgtacaaag aattccctcc tcctccgcca ccgccaccac cgccgccgcc gccaccacca
     1381 ggaggatctg gcggatctcc tcctccgcct cctccaccac caccgccacc gcctccacca
     1441 cctggcggcc gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1501 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
     1561 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1621 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
     1681 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1741 cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1801 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1861 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
     1921 aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcagc
     1981 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2041 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2101 gatcacatgg tcctgctgga gttcgtgacc gccgccacta gtgccggagg catgtcccat
     2161 cactgggggt acggcaaaca caacggacct gagcactggc ataaggactt ccccattgcc
     2221 aagggagagc gccagtcccc tgttgacatc gacactcata cagccaagta tgacccttcc
     2281 ctgaagcccc tgtctgtttc ctatgatcaa gcaacttccc tgaggatcct caacaatggt
     2341 catgctttca acgtggagtt tgatgactct caggacaaag cagtgctcaa gggaggaccc
     2401 ctggatggca cttacagatt gattcagttt cactttcact ggggttcact tgatggacaa
     2461 ggttcagagc atactgtgga taaaaagaaa tatgctgcag aacttcactt ggttcactgg
     2521 aacaccaaat atggggattt tgggaaagct gtgcagcaac ctgatggact ggccgttcta
     2581 ggtatttttt tgaaggttgg cagcgctaaa ccgggccttc agaaagttgt tgacgtgctg
     2641 gattccatta aaacaaaggg caagagtgct gacttcacta acttcgatcc tcgtggcctc
     2701 cttcctgaat ccctggatta ctggacctac ccaggctcac tgaccacccc tcctcttctg
     2761 gaatgtgtga cctggattgt gctcaaggaa cccatcagcg tcagcagcga gcaggtgttg
     2821 aaattccgta aacttaactt caatggggag ggtgaacccg aagaactgat ggtggacaac
     2881 tggcgcccag ctcagccact gaagaacagg caaatcaaag cttccttcaa acgagctccg
     2941 ggcttctcct caatttccgc tcatcaccac catcatcacc atcaccacca ctaactcgag
     3001 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3061 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3121 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3181 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggctagag
     3241 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     3301 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     3361 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     3421 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     3481 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     3541 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     3601 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     3661 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     3721 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     3781 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     3841 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     3901 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     3961 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     4021 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     4081 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     4141 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     4201 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     4261 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     4321 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     4381 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     4441 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     4501 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     4561 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     4621 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     4681 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     4741 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     4801 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     4861 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     4921 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     4981 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     5041 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     5101 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5161 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5221 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5281 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5341 ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta
     5401 gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc
     5461 gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc
     5521 taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga
     5581 gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg
     5641 ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga
     5701 tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg
     5761 gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct
     5821 tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac
     5881 cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta
     5941 ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata
     6001 tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt
     6061 gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt
     6121 ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat
     6181 acgaacacac cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc
     6241 aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca
     6301 cgatgctttt ccaaaccacc ctcctttttt gcgccctgcc tccatcaccc tgaccccggg
     6361 gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc gctcccgggg
     6421 gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa tcggcttccc
     6481 ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga gacccggatg
     6541 atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga cctctccccc
     6601 tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg cggcctccac
     6661 tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg cctcctcgac
     6721 cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc
     6781 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
     6841 ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc
     6901 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
     6961 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg
     7021 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcctg
     7081 cccctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     7141 cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
     7201 ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg
     7261 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
     7321 cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     7381 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct gctcctgctc
     7441 ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga
     7501 caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc
     7561 ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc
     7621 cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag
     7681 gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt
     7741 tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc
     7801 ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc
     7861 tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca
     7921 tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg
     7981 ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac
     8041 agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc
     8101 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
     8161 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     8221 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8281 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8341 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8401 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8461 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8521 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     8581 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8641 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8701 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8761 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8821 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     8881 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8941 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9001 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9061 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9121 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9181 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9241 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9301 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9361 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9421 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9481 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     9541 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9601 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9661 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9721 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9781 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9841 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9901 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9961 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt
    10021 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag
    10081 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgaactagat gatccggctg
    10141 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    10201 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    10261 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
    10321 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    10381 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    10441 tgaggaggct tttttggagg gtgaccgcca cgaccggtgc cgccaccatc ccctgaccca
    10501 cgcccctgac ccctcacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc
    10561 ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac
    10621 taccccgcca cgcgccacac cgtcgacccc gaccgccaca tcgaacgcgt caccgagctg
    10681 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
    10741 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
    10801 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
    10861 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
    10921 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
    10981 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
    11041 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
    11101 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
    11161 accgaaagga gcgcacgacc cggtccgacg cggcccgacg ggtccgaggg gggtcgacct
    11221 cgaaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    11281 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    11341 tcttatcatg tctggatctg atccgaaccc cttcctcgac caattctcat gtttgacagc
    11401 ttatcatcgc agatccgggc aacgttgttg ccattgctcg accaattctc atgtttgaca
    11461 gcttatcatc gcagatccgt atggtgcact ctcagtacaa tctgctctga tgccgcatag
    11521 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    11581 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    11641 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtatctg aggggactag
    11701 ggtgtgttta ggcgaaaagc ggggcttcgg ttgtacgcgg ttaggagtcc cctcaggata
    11761 tagtagtttc gcttttgcat agggaggggg aaatgtagtc ttatgcaata ctcttgtagt
    11821 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
    11881 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
    11941 gtctgacatg gattggacga accactgaat tccgcattgc agagatattg tatttaagtg
    12001 cctagctcga tacaataaac gccatttgac cattcaccac attggtgtgc acctccaagc
    12061 tggccgggga tcctctaggg cctctgagct attccagaag tagtgaagag gcttttttgg
    12121 aggcctaggc ttttgcaaaa agctccggat cgatcctgag aacttcaggg tgagtttggg
    12181 gacccttgat tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa
    12241 agttttcagg gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg
    12301 ataattttgt ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt
    12361 ttcattttct gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt
    12421 cacttttgtt tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa
    12481 tcagggtata ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg
    12541 ataaggtaga atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag
    12601 aaacaactac atcctggtca tcatcctgcc tttctcttta tggttacaat gatatacact
    12661 gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca
    12721 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
    12781 ttttggcaaa gaattgtaat acgactcact atagggcgaa ttgatatgtc tagattagat
    12841 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta
    12901 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta
    12961 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact
    13021 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt
    13081 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct
    13141 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt
    13201 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta
    13261 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt
    13321 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc
    13381 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt
    13441 gggtccgcgt acagcggatc ccgggaattc agatcttatt aaagcagaac ttgtttattg
    13501 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    13561 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt
    13621 cgaatccatg gcgcagaact ggtaggtatg gaagatccgc gttgacattg attattgact
    13681 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
    13741 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
    13801 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
    13861 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
    13921 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
    13981 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
    14041 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
    14101 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
    14161 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
    14221 cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt
    14281 gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
    14341 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt
    14401 aaacttaag
//
