LOCUS       sequence-243226-       13494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eukaryotic expression of the fusion protein used for drug diruption 
            the p53-HDM2 interaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13494)
  AUTHORS   Scarabelli S, Tan KT, Griss R, Hovius R, D'Alessandro PL, Vorherr T,
            Johnsson K
  TITLE     Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins.
  JOURNAL   ACS Sens. 2017 Aug 25;2(8):1191-1197. doi: 
            10.1021/acssensors.7b00331. Epub 2017 Aug 2.
  PUBMED    28766337
REFERENCE   2  (bases 1 to 13494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.7b00331"; journalName: "ACS Sens"; date: 
            "2017-08-25- 25"; volume: "2"; issue: "8"; pages: "1191-1197"
COMMENT     Sequence Label: pEBTet-p52-HDM2_sensor
FEATURES             Location/Qualifiers
     source          1..13494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     193..212
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     240..259
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        240..258
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             269..889
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(342..361)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    931..1052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        1107..1486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1487..1690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1641..1661
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1692..1710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1713..1731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1741..1765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1865..1888
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1895..1909
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             1967..2680
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     CDS             join(2804..2806,2807..2809,2810..3322)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(2849..2870)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3110..3129)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3457..3478
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3899..3928
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(3969..3986)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3975..4086
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4627..6416
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9080..9098)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9166..9270
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9271..9339,9340..10131)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9489..9508)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10302..10890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10791..10810
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(11079..11099)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11097..11394
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             11471..12070
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11471..11490)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11967..11987
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    12161..12295
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12433..12452)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        12729..12990
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          join(13107..13494,1..185)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg
       61 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa
      121 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc
      181 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgt
      241 aatacgactc actatagggc gaattgatat gtctagatta gataaaagta aagtgattaa
      301 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
      361 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
      421 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
      481 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
      541 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
      601 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
      661 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
      721 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
      781 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
      841 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg cgtacagcgg
      901 atcccgggaa ttcagatctt attaaagcag aacttgttta ttgcagctta taatggttac
      961 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1021 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggtcgaatcc atggcgcaga
     1081 actggtaggt atggaagatc cgcgttgaca ttgattattg actagttatt aatagtaatc
     1141 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1201 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1261 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1321 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1381 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1441 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     1501 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     1561 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     1621 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     1681 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
     1741 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     1801 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttaccat
     1861 ggcctggagc cacccgcagt tcgaaaaggg tgcagatgac gacgacaagg taccgagcca
     1921 ggaaaccttt agcgatctgt ggaaactgct gccggaaaat agtactatgt ctaaaggtga
     1981 agaattattc actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca
     2041 caaattttct gtctccggtg aaggtgaagg tgatgctacg tacggtaaat tgaccttaaa
     2101 attactctgt actactggta aattgccagt tccatggcca accttagtca ctactttagg
     2161 ttatggtgtt caatgttttg ctagataccc agatcatatg aaacaacatg actttttcaa
     2221 gtctgccatg ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa
     2281 ctacaagacc agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt
     2341 aaaaggtatt gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta
     2401 taactctcac aatgtttaca tcactgctga caaacaaaag aatggtatca aagctaactt
     2461 caaaattaga cacaacattg aagatggtgg tgttcaatta gctgaccatt atcaacaaaa
     2521 tactccaatt ggtgatggtc cagtcttgtt accagacaac cattacttat cctatcaatc
     2581 tgccttattc aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttttgac
     2641 tgctgctggt attaccgagg gtatgaatga attgtacaaa gaattccctc ctcctccgcc
     2701 accgccacca ccgccgccgc cgccaccacc aggaggatct ggcggatctc ctcctccgcc
     2761 tcctccacca ccaccgccac cgcctccacc acctggcggc cgcatggtga gcaagggcga
     2821 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2881 caggttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2941 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3001 ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3061 gtccgccatg cccgaaggct acgtccagga gcgtaccatc ttcttcaagg acgacggcaa
     3121 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3181 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3241 catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggcccactt
     3301 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3361 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3421 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3481 cgccgccact agtatgtgca ataccaacat gtctgtacct actgatggtg ctgtaaccac
     3541 ctcacagatt ccagcttcgg aacaagagac cctggttaga ccaaagccat tgcttttgaa
     3601 gttattaaag tctgttggtg cacaaaaaga cacttatact atgaaagagg ttctttttta
     3661 tcttggccag tatattatga ctaaacgatt atatgatgag aagcaacaac atattgtata
     3721 ttgttcaaat gatcttctag gagatttgtt tggcgtgcca agcttctctg tgaaagagca
     3781 caggaagata tataccatga tctacaggaa cttggtagta gtcaatcagc aggaatcatc
     3841 ggactcaggt acatctgtga gtgagaaccg agctccgggc ttctcctcaa tttccgctca
     3901 tcaccaccat catcaccatc accaccacta actcgagtct agagggcccg tttaaacccg
     3961 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4021 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4081 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4141 caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc cccggacgaa
     4201 ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt aatcccttca
     4261 gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa
     4321 acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca
     4381 acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt
     4441 gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc
     4501 caggggccca ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc
     4561 taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta
     4621 accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta
     4681 attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag
     4741 ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg
     4801 gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa
     4861 ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct
     4921 aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt
     4981 ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat
     5041 gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca
     5101 ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg
     5161 tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg
     5221 gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct
     5281 atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct
     5341 aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg
     5401 gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct
     5461 gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca
     5521 ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa
     5581 gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac
     5641 acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga
     5701 gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat
     5761 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     5821 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     5881 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     5941 gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat
     6001 gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta
     6061 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta
     6121 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     6181 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     6241 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     6301 tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt
     6361 gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg
     6421 ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg
     6481 ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga
     6541 gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca
     6601 agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt
     6661 agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca
     6721 ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc
     6781 accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc
     6841 gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag
     6901 gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa
     6961 ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc
     7021 aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg
     7081 ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt
     7141 tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc
     7201 aatgttctca aatttcgggt tggaacctcc ttgaccacga tgcttttcca aaccaccctc
     7261 cttttttgcg ccctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct
     7321 gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg
     7381 ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt
     7441 ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc
     7501 ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc
     7561 ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca
     7621 ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct
     7681 gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc
     7741 cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
     7801 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
     7861 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     7921 gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct
     7981 cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgctcct
     8041 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     8101 cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc
     8161 cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     8221 gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct
     8281 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
     8341 cctcctgctc ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg
     8401 cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg tcttggccct
     8461 gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt
     8521 cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt
     8581 ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca
     8641 gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata
     8701 cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc
     8761 gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc
     8821 accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc
     8881 ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc
     8941 tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga
     9001 agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag
     9061 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     9121 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     9181 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     9241 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     9301 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     9361 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     9421 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     9481 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     9541 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9601 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9661 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9721 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9781 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     9841 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9901 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9961 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    10021 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    10081 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    10141 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    10201 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    10261 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    10321 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    10381 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    10441 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    10501 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    10561 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10621 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10681 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10741 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10801 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10861 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10921 ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc
    10981 atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag
    11041 gccatccagc ctcgcgtcga actagatgat ccggctgtgg aatgtgtgtc agttagggtg
    11101 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    11161 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    11221 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    11281 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    11341 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggagggtg
    11401 accgccacga ccggtgccgc caccatcccc tgacccacgc ccctgacccc tcacaaggag
    11461 acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
    11521 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
    11581 cgaccccgac cgccacatcg aacgcgtcac cgagctgcaa gaactcttcc tcacgcgcgt
    11641 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
    11701 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
    11761 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
    11821 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
    11881 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
    11941 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    12001 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    12061 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccgg
    12121 tccgacgcgg cccgacgggt ccgagggggg tcgacctcga aacttgttta ttgcagctta
    12181 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    12241 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc
    12301 cgaacccctt cctcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
    12361 gttgttgcca ttgctcgacc aattctcatg tttgacagct tatcatcgca gatccgtatg
    12421 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    12481 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
    12541 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
    12601 tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc gaaaagcggg
    12661 gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg
    12721 gagggggaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
    12781 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
    12841 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
    12901 actgaattcc gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacgcc
    12961 atttgaccat tcaccacatt ggtgtgcacc tccaagctgg ccggggatcc tctagggcct
    13021 ctgagctatt ccagaagtag tgaagaggct tttttggagg cctaggcttt tgcaaaaagc
    13081 tccggatcga tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt
    13141 tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa
    13201 tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc
    13261 tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt
    13321 taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt
    13381 gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt
    13441 cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata tttc
//
