LOCUS       sequence-243229-         569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression for affinity purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Murthy AC, Dignon GL, Kan Y, Zerze GH, Parekh SH, Mittal J, Fawzi NL
  TITLE     Molecular interactions underlying liquid-liquid phase separation of 
            the FUS low-complexity domain.
  JOURNAL   Nat Struct Mol Biol. 2019 Jul;26(7):637-648. doi: 
            10.1038/s41594-019-0250-x. Epub 2019 Jul 1.
  PUBMED    31270472
REFERENCE   2  (bases 1 to 569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-019-0250-x"; journalName: "Nat Struct Mol Biol"; 
            date: "2019-07"; volume: "26"; issue: "7"; pages: "637-648"
COMMENT     Sequence Label: RP1B FUS 1-163 S12xQ
FEATURES             Location/Qualifiers
     source          1..569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaaggagata taccatgggc tctgataaaa ttcatcacca tcaccatcac gaaaatcttt
       61 attttcaagg tatggcctca aacgattata cccaacaagc aacccaaagc tatggggcct
      121 accccaccca gcccgggcag ggctattccc agcagagcca gcagccctac ggacagcaga
      181 gttacagtgg ttatagccag cagacggaca ctcagggcta tggccagcag agctattctt
      241 cttatggcca gagccagaac acaggctatg gaactcagca gactccccag ggatatggct
      301 cgactggcgg ctatggcagt cagcagagct cccaacagtc ttacgggcag cagcagtcct
      361 atcctggcta tggccagcag ccagctccca gccagacctc gggaagttac ggtagcagtc
      421 agcagcagag cagctatggg cagccccagc aggggagcta cagccagcag cctagctatg
      481 gtggacagca gcaaagctat ggacagcagc aaagctataa tccccctcag ggctatggac
      541 agcagaacca gtacaacagc tgactcgag
//
