LOCUS       sequence-243231-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression for affinity purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Murthy AC, Dignon GL, Kan Y, Zerze GH, Parekh SH, Mittal J, Fawzi NL
  TITLE     Molecular interactions underlying liquid-liquid phase separation of 
            the FUS low-complexity domain.
  JOURNAL   Nat Struct Mol Biol. 2019 Jul;26(7):637-648. doi: 
            10.1038/s41594-019-0250-x. Epub 2019 Jul 1.
  PUBMED    31270472
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-019-0250-x"; journalName: "Nat Struct Mol Biol"; 
            date: "2019-07"; volume: "26"; issue: "7"; pages: "637-648"
COMMENT     Sequence Label: RP1B FUS 1-163 QQ4xSS #1
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(110..121)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(502..522)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(523..540)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             565..587
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    602..626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(626..645)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(627..645)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcgaggatct cagctgttgt actggttgct gcttccatag ccctgagggg gattatagct
       61 ttgctgctgt ccatagcttt gctgctgtcc accatagcta gggctgctgc tgtagctccc
      121 actctggggc tgcccatagc tgctgctctg agaactgcta ccgtaacttc ccgaggtgct
      181 gctgggagct ggctgctggc catagccagg ataggaggag ctgctcccgt aagacgattg
      241 ggagctctgg ctactgccat agccgccagt cgagccatat ccctggggag ttgactgagt
      301 tccatagcct gtgttctggc tctggccata agaagaatag ctgctctggc catagcctga
      361 agtgtccgtg gactggctat aaccactgta actctgctgt ccgtagggct gactgctgct
      421 gctggaatag ccctgcccgg gctgggtggg gtaggcccca tagctttggg ttgcttgttg
      481 ggtataatcg tttgaggcca taccttgaaa ataaagattt tcgtgatgat gatgatgatg
      541 aattttatca gagcccatgg tatatctcct tcttaaagtt aaacaaaatt atttctagag
      601 gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga
      661 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcc
//
