LOCUS       sequence-243232-         569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression for affinity purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Murthy AC, Dignon GL, Kan Y, Zerze GH, Parekh SH, Mittal J, Fawzi NL
  TITLE     Molecular interactions underlying liquid-liquid phase separation of 
            the FUS low-complexity domain.
  JOURNAL   Nat Struct Mol Biol. 2019 Jul;26(7):637-648. doi: 
            10.1038/s41594-019-0250-x. Epub 2019 Jul 1.
  PUBMED    31270472
REFERENCE   2  (bases 1 to 569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-019-0250-x"; journalName: "Nat Struct Mol Biol"; 
            date: "2019-07"; volume: "26"; issue: "7"; pages: "637-648"
COMMENT     Sequence Label: RP1B FUS 1-163 QQ4xSS #2
FEATURES             Location/Qualifiers
     source          1..569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             452..463
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gaaggagata taccatgggc tctgataaaa ttcatcacca tcaccatcac gaaaatcttt
       61 attttcaagg tatggcctca aacgattata ccagcagcgc aacccaaagc tatggggcct
      121 accccaccca gcccgggcag ggctattccc agcagagcag tcagccctac ggacagcaga
      181 gttacagtgg ttatagccag tccacggaca cttcaggcta tggccagagc agctattctt
      241 cttatggcag cagcagcaac acaggctatg gaactcagtc aactccccag ggatatggct
      301 cgactggcgg ctatggcagt agccagagct cccaatcgtc ttacgggcag cagtcctcct
      361 atcctggcta tggcagcagc ccagctccca gcagcacctc gggaagttac ggtagcagtt
      421 ctcagagcag cagctatggg cagccccaga gtgggagcta cagccagcag cctagctatg
      481 gtggaagcag ccaaagctat ggacagcagc aaagctataa tccccctcag ggctatggac
      541 agcagaacca gtacaacagc tgactcgag
//
