LOCUS       sequence-243242-       12916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive GFP expression, WUS2, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM119
FEATURES             Location/Qualifiers
     source          1..12916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      889..1083
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1319..1341)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1427..1567
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1482..1501
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1582..1599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1753..2341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1833..1852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2428..3222)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3647..3671
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4612..4957
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4900..4920
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(5008..5036)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5631..5651
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      5702..5950
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        6440..6515
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6618..6797
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6650..6833
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    10285..10309
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11609..12238
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(12672..12916,1..823)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
ORIGIN
        1 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
       61 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
      121 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
      181 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
      241 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
      301 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
      361 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
      421 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
      481 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
      541 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
      601 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
      661 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
      721 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
      781 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
      841 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac
      901 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
      961 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     1021 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     1081 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     1141 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     1201 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     1261 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     1321 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca
     1381 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     1441 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     1501 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     1561 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1741 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2161 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2401 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
     2461 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     2521 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     2581 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
     2641 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
     2701 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
     2761 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
     2821 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
     2881 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
     2941 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
     3001 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
     3061 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
     3121 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
     3181 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
     3241 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
     3301 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
     3361 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
     3421 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     3481 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     3541 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
     3601 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
     3661 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
     3721 agagctcaaa gtttaacgcg ttagcagaag gcatgttgtt gtgactccga ggggttgcct
     3781 caaactctat cttataaccg gcgtggaggc atggaggcag gggtattttg gtcattttaa
     3841 tagatagtgg aaaatgacgt ggaatttact taaagacgaa gtctttgcga caaggggggg
     3901 cccacgccga atttaatatt accggcgtgg ccccccctta tcgcgagtgc tttagcacga
     3961 gcggtccaga tttaaagtag aaaatttccc gcccactagg gttaaaggtg ttcacactat
     4021 aaaagcatat acgatgtgat ggtatttgat ggagcgtata ttgtatcagg tatttccgtt
     4081 ggatacgaat tattcgtacg accctcggta ccgatcggcg cgccagattt gccttttcaa
     4141 tttcagaaag aatgctaacc cacagatggt tagagaggct tacgcagcag gtatcatcaa
     4201 gacgatctac ccgagcaata atctccagga aatcaaatac cttcccaaga aggttaaaga
     4261 tgcagtcaaa agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa
     4321 gatcagaagt actattccag tatggacgat tcaaggcttg cttcacaaac caaggcaagt
     4381 aatagagatt ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa
     4441 gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag
     4501 tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact
     4561 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
     4621 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
     4681 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
     4741 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     4801 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     4861 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     4921 ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactcctg caggatggtg
     4981 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     5041 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     5101 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aaccctggtc
     5161 accaccctga cctacggcgt gcagtgcttc tcccgttacc ctgatcatat gaagcggcac
     5221 gacttcttca agagcgccat gcctgaggga tacgtgcagg agaggaccat cttcttcaag
     5281 gacgacggga actacaagac acgtgctgaa gtcaagtttg agggagacac cctcgtcaac
     5341 aggatcgagc ttaagggaat cgatttcaag gaggacggaa acatcctcgg ccacaagttg
     5401 gaatacaact acaactccca caacgtatac atcatggccg acaagcaaaa gaacggcatc
     5461 aaagccaact tcaagacccg ccacaacatc gaagacggcg gcgtgcaact cgctgatcat
     5521 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     5581 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     5641 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataactcgag
     5701 atatgaagat gaagatgaaa tatttggtgt gtcaaataaa aagcttgtgt gcttaagttt
     5761 gtgttttttt cttggcttgt tgtgttatga atttgtggct ttttctaata ttaaatgaat
     5821 gtaagatcac attataatga ataaacaaat gtttctataa tccattgtga atgttttgtt
     5881 ggatctcttc tgcagcatat aactactgta tgtgctatgg tatggactat ggaatatgat
     5941 taaagataag gagctccggt gacggaccca tggcttcgtt gaacaacgga aactcgactt
     6001 gccttccgca caatacatca tttcttctta gctttttttc ttcttcttcg ttcatacagt
     6061 ttttttttgt ttatcagctt acattttctt gaaccgtagc tttcgttttc ttctttttaa
     6121 ctttccattc ggagtttttg tatcttgttt catagtttgt cccaggatta gaatgattag
     6181 gcatcgaacc ttcaagaatt tgattgaata aaacatcttc attcttaaga tatgaagata
     6241 atcttcaaaa ggcccctggg aatctgaaag aagagaagca ggcccattta tatgggaaag
     6301 aacaatagta tttcttatat aggcccattt aagttgaaaa caatcttcaa aagtcccaca
     6361 tcgcttagat aagaaaacga agctgagttt atatacagct agagtcgaag tagtgattgt
     6421 tggtagtagc gactccatgg ttttagagct agaaatagca agttaaaata aggctagtcc
     6481 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttcccgggcg cgccgatcat
     6541 gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt
     6601 tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg ggtttctgga
     6661 gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag
     6721 gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc
     6781 ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct gcaccgtacc
     6841 tgcagggtcc gagctaggtc acagaagcgc tcaggaaggc cgctgagata gaggcatggc
     6901 ggccaatgcg ggcggcggtg gagcgggagg aggcagcggc agcggcagcg tggctgcgcc
     6961 ggcggtgtgc cgccccagcg gctcgcggtg gacgccgacg ccggagcaga tcaggatgct
     7021 gaaggagctg tactacggct gcggcatccg gtcgcccagc tcggagcaga tccagcgcat
     7081 caccgccatg ctgcggcagc acggcaagat cgagggcaag aacgtcttct actggttcca
     7141 gaaccacaag gcccgcgagc gccagaagcg ccgcctcacc agcctcgacg tgaacgtgcc
     7201 cgccgccggc gcggccgacg ccaccaccag ccaactcggc gtcctctcgc tgtcgtcgcc
     7261 gccgccttca ggcgcggcgc ctccctcgcc caccctcggc ttctacgccg ccggcaatgg
     7321 cggcggatcg gctgtgctgc tggacacgag ttccgactgg ggcagcagcg gcgctgcgat
     7381 ggccaccgag acatgcttcc tccaggacta catgggcgtg acggacacgg gcagctcgtc
     7441 gcagtggcca cgcttctcgt cgtcggacac gataatggcg gcggccgcgg cgcgggcggc
     7501 gacgacgcgg gcgcccgaga ctctccctct cttcccgacc tgcggcgacg acggcggcag
     7561 cggtagcagc agctacttgc cgttctgggg tgccgcgtcc acaactgccg gcgccacttc
     7621 ttccgttgcg atccagcagc aacaccagct gcaggagcag tacagctttt acagcaacag
     7681 caacagcacc cagctggccg gcaccggcaa ccaagacgta tcggcaacag cagcagcagc
     7741 cgccgccctg gagctgagcc tcagctcatg gtgctcccct taccctgctg cagggagtat
     7801 gtgagagcaa cgcgagctgc cactgctctt cacttatgtc tctggaatgg aaggaggagg
     7861 aagtgagcat agcgttggtg cgttgctgtc attgtcctag gttagtagct agtgccagtt
     7921 actagtaagc atcaggcata ggagtatgta gtagaagcat gcacgttgcc ggccagccag
     7981 gctttagacg ggaaaagaat ttggtgcagc cggctgcaaa acaggatgtt tacagccccc
     8041 ccctcgagcc ctagacttgt ccatcttctg gattggccaa gttaattaat gtatgaaata
     8101 aaaggatgca cacatagtga catgctaatc actataatgt gggcatcaaa gttgtgtgtt
     8161 atgtgtaatt actaattatc tgaataagag aaagagatca tccatatttc ttatcctaaa
     8221 tgaatgtcac gtgtctttat aattctttga tgaaccagat gcattttatt aaccaattcc
     8281 atatacatat aaatattaat catatataat taatatcaat tgggttagca aaacaaatct
     8341 agtctaggtg tgttttgcta attattgggg gatagtgcaa aaagaaatct acgttctcaa
     8401 taattcagat agaaaactta ataaagtgag ataatttaca tagattgctt ttatcctttg
     8461 atatatgtga aaccatgcat gatataagga aaatagatag agaaataatt ttttacatcg
     8521 ttgaatatgt aaacaattta attcaagaag ctaggaatat aaatattgag gagtttatga
     8581 ttagagctct cccggcgcgc cgatatcgag ctcagtgttt gatcgccggc ggtaccgagt
     8641 gtacttcaag tcagtgggaa atcaataaaa tgattatttt atgaatatat ttcattgtgc
     8701 aagtagatag aaattacata tgttacataa cacacgaaat aaacaaaaaa agacaatcca
     8761 aaaacaaaca ccccaaaaaa aataatcact ttagataaac tcgtatgagg agaggcacgt
     8821 tcagtgactc gacgattccc gagcaaaaaa agtctccccg tcacacatgt agtgggtgac
     8881 gcaattatct ttaaagtaat ccttctgttg acttgtcatt gataacatcc agtcttcgtc
     8941 aggattgcaa agaattatag aagggatccc accttttatt ttcttctttt ttccatattt
     9001 agggttgaca gtgaaatcag actggcaacc tattaattgc ttccacaatg ggacgaactt
     9061 gaaggggatg tcgtcgatga tattataggt ggcgtgttca tcgtagttgg tgaaatcgat
     9121 ggtaccgttc caatagttgt gtcgtccgag acttctagcc caggtggtct ttccggtacg
     9181 agttggtccg cagatgtaga ggctggggtg tcggattcca ttccttccat tgtccttgtt
     9241 aaatcggcca tccattcaag gtcagattga gcttgttggt atgagacagg atgtatgtaa
     9301 gtataagcgt ctatgcttac atggtataga tgggtttccc tccaggagtg tagatcttcg
     9361 tggcagcgaa gatctgattc tgtgaagggc gacacatacg gttcaggttg tggagggaat
     9421 aatttgttgg ctgaatattc cagccattga agctttgttg cccattcatg agggaattct
     9481 tccttgatca tgtcaagata ttcctcctta gacgttgcag tctggataat agttctccat
     9541 cgtgcgtcag atttgcgagg agaaacctta tgatctcgga aatctcctct ggttttaata
     9601 tctccgtcct ttgatatgta atcaaggact tgtttagagt ttctagctgg ctggatatta
     9661 gggtgatttc cttcaaaatc gaaaaaagaa ggatccctaa tacaaggttt tttatcaagc
     9721 tggagaagag catgatagtg ggtagtgcca tcttgatgaa gctcagaagc aacaccaagg
     9781 aagaaaataa gaaaaggtgt gagtttctcc cagagaaact ggaataaatc atctctttga
     9841 gatgagcact tgggataggt aaggaaaaca tatttagatt ggagtctgaa gttcttacta
     9901 gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt ataaccggcg
     9961 tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa atgacgtgga
    10021 atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt taatattacc
    10081 ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt aaagtagaaa
    10141 atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg atgtgatggt
    10201 atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat tcgtacgacc
    10261 ctcatagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
    10321 gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
    10381 tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc
    10441 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
    10501 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
    10561 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
    10621 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
    10681 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
    10741 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
    10801 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
    10861 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
    10921 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
    10981 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
    11041 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
    11101 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
    11161 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
    11221 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
    11281 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
    11341 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
    11401 ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
    11461 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
    11521 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
    11581 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
    11641 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
    11701 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
    11761 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
    11821 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
    11881 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
    11941 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
    12001 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
    12061 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
    12121 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
    12181 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
    12241 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
    12301 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
    12361 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
    12421 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
    12481 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
    12541 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
    12601 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
    12661 atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
    12721 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
    12781 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
    12841 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
    12901 ttttcgttcc gatgct
//
