LOCUS       sequence-243244-       13546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, IPT, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 13546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM134
FEATURES             Location/Qualifiers
     source          1..13546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      841..1035
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1271..1293)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1379..1519
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1434..1453
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1534..1551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1705..2293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1785..1804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2380..3174)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3599..3623
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4550..6202
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4616..4634)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      6210..6458
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        6948..7023
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7539..7884
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7827..7847
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    10867..10891
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12191..12820
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(13254..13546,1..775)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
ORIGIN
        1 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
       61 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
      121 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
      181 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
      241 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
      301 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
      361 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
      421 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
      481 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
      541 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
      601 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
      661 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
      721 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
      781 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gcctctttcc
      841 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
      901 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      961 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     1021 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     1081 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     1141 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     1201 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     1261 acggtgaaaa cctctgacac atgcagctcc cggaaacggt cacagcttgt ctgtaagcgg
     1321 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     1381 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     1441 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     1501 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1561 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1621 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1681 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1741 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1801 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1861 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1921 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1981 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2041 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2101 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     2161 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2221 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2281 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2341 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa
     2401 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
     2461 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
     2521 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
     2581 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
     2641 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
     2701 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
     2761 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
     2821 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
     2881 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
     2941 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
     3001 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
     3061 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
     3121 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
     3181 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
     3241 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
     3301 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
     3361 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
     3421 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
     3481 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
     3541 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
     3601 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
     3661 cgtttttaat gtagagctca aagtttaacg cgttagcaga aggcatgttg ttgtgactcc
     3721 gaggggttgc ctcaaactct atcttataac cggcgtggag gcatggaggc aggggtattt
     3781 tggtcatttt aatagatagt ggaaaatgac gtggaattta cttaaagacg aagtctttgc
     3841 gacaaggggg ggcccacgcc gaatttaata ttaccggcgt ggccccccct tatcgcgagt
     3901 gctttagcac gagcggtcca gatttaaagt agaaaatttc ccgcccacta gggttaaagg
     3961 tgttcacact ataaaagcat atacgatgtg atggtatttg atggagcgta tattgtatca
     4021 ggtatttccg ttggatacga attattcgta cgaccctcgg taccgatcgg cgcgcctggc
     4081 agacatactg tcccacaaat gaagatggaa tctgtaaaag aaaacgcgtg aaataatgcg
     4141 tctgacaaag gttaggtcgg ctgcctttaa tcaataccaa agtggtccct accacgatgg
     4201 aaaaactgtg cagtcggttt ggctttttct gacgaacaaa taagattcgt ggccgacagg
     4261 tgggggtcca ccatgtgaag gcatcttcag actccaataa tggagcaatg acgtaagggc
     4321 ttacgaaata agtaagggta gtttgggaaa tgtccactca cccgtcagtc tataaatact
     4381 tagcccctcc ctcattgtta agggagcaaa atctcagaga gatagtccta gagagagaaa
     4441 gagagcaagt agcctagaag tagtcaaggc ggcgaagtat tcaggcacgt ggccaggaag
     4501 aagaaaagcc aagacgacga aaacaggtaa gagctaagct tcctgcacca tggaagacgc
     4561 caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga
     4621 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
     4681 tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc
     4741 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa
     4801 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc
     4861 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac
     4921 cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc
     4981 aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat
     5041 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga
     5101 gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct
     5161 gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga
     5221 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca
     5281 tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt
     5341 aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag
     5401 tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata
     5461 cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg
     5521 ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga
     5581 gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa
     5641 agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt
     5701 taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa
     5761 tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc
     5821 ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta
     5881 caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat
     5941 cttcgacgct ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt
     6001 tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag
     6061 tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa
     6121 aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa
     6181 gggcggaaag atcgccgtgt aactcgagat atgaagatga agatgaaata tttggtgtgt
     6241 caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat
     6301 ttgtggcttt ttctaatatt aaatgaatgt aagatcacat tataatgaat aaacaaatgt
     6361 ttctataatc cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg
     6421 tgctatggta tggactatgg aatatgatta aagataagga gctccggtga cggacccatg
     6481 gcttcgttga acaacggaaa ctcgacttgc cttccgcaca atacatcatt tcttcttagc
     6541 tttttttctt cttcttcgtt catacagttt ttttttgttt atcagcttac attttcttga
     6601 accgtagctt tcgttttctt ctttttaact ttccattcgg agtttttgta tcttgtttca
     6661 tagtttgtcc caggattaga atgattaggc atcgaacctt caagaatttg attgaataaa
     6721 acatcttcat tcttaagata tgaagataat cttcaaaagg cccctgggaa tctgaaagaa
     6781 gagaagcagg cccatttata tgggaaagaa caatagtatt tcttatatag gcccatttaa
     6841 gttgaaaaca atcttcaaaa gtcccacatc gcttagataa gaaaacgaag ctgagtttat
     6901 atacagctag agtcgaagta gtgattgttg gtagtagcga ctccatggtt ttagagctag
     6961 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     7021 tgcttttttt cccggcgtaa tatggcgcgc cagatttgcc ttttcaattt cagaaagaat
     7081 gctaacccac agatggttag agaggcttac gcagcaggta tcatcaagac gatctacccg
     7141 agcaataatc tccaggaaat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
     7201 ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact
     7261 attccagtat ggacgattca aggcttgctt cacaaaccaa ggcaagtaat agagattgga
     7321 gtctctaaaa aggtagttcc cactgaatca aaggccatgg agtcaaagat tcaaatagag
     7381 gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc
     7441 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa
     7501 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta
     7561 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata
     7621 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt
     7681 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     7741 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
     7801 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     7861 agttcatttc atttggagag aacacggggg actcctgcag gatggatctg cgtctaattt
     7921 tcggtccaac ttgcacagga aagacgtcga ccgcgatacg tcttgcccag cagactggcc
     7981 ttccagtcct ttcgctcgat cgggtccaat gctgtcctca actgtcaacc ggaagcggac
     8041 gaccaacagt ggaagaactg aaaggaacga cccgtctata ccttgaagat cggcctctgg
     8101 tgaagggtat catcgcagcc aagcaagctc acgaaaggct gatcggggaa gtgtacaatt
     8161 atgaggccca cggcgggctt attcttgagg gaggatctat ctcgttgctc aggtgcatgg
     8221 cgcaaagcag ttattggagt accgattttc gttggcatat tattcgccac aagttagcag
     8281 acgaggagac attcatgaac gcggccaagg ccagagttag gcagatgttg cgccctgctg
     8341 taggcccatc tattattcaa gagttggttc atctttggaa tgagcctcgg ctgaggccca
     8401 tactgaaaga gatcgacgga tatcgatatg ccatgttatt tgctagccag aaccagatca
     8461 cacccgatat gctattgcag cttgacccag atatggaggg tgagttgatt catggaatcg
     8521 ctcaggagta tctcatccat gcgcgccggc aggagcagga attccctcca gtgagcgtgg
     8581 tcgctttcga aggattcgaa ggtccaccgt tcggaatgtg ctagctcgag ccctagactt
     8641 gtccatcttc tggattggcc aagttaatta atgtatgaaa taaaaggatg cacacatagt
     8701 gacatgctaa tcactataat gtgggcatca aagttgtgtg ttatgtgtaa ttactaatta
     8761 tctgaataag agaaagagat catccatatt tcttatccta aatgaatgtc acgtgtcttt
     8821 ataattcttt gatgaaccag atgcatttta ttaaccaatt ccatatacat ataaatatta
     8881 atcatatata attaatatca attgggttag caaaacaaat ctagtctagg tgtgttttgc
     8941 taattattgg gggatagtgc aaaaagaaat ctacgttctc aataattcag atagaaaact
     9001 taataaagtg agataattta catagattgc ttttatcctt tgatatatgt gaaaccatgc
     9061 atgatataag gaaaatagat agagaaataa ttttttacat cgttgaatat gtaaacaatt
     9121 taattcaaga agctaggaat ataaatattg aggagtttat gattagagct ctcccggcgc
     9181 gccgatatcg agctcagtgt ttgatcgccg gcggtaccga gtgtacttca agtcagtggg
     9241 aaatcaataa aatgattatt ttatgaatat atttcattgt gcaagtagat agaaattaca
     9301 tatgttacat aacacacgaa ataaacaaaa aaagacaatc caaaaacaaa caccccaaaa
     9361 aaaataatca ctttagataa actcgtatga ggagaggcac gttcagtgac tcgacgattc
     9421 ccgagcaaaa aaagtctccc cgtcacacat gtagtgggtg acgcaattat ctttaaagta
     9481 atccttctgt tgacttgtca ttgataacat ccagtcttcg tcaggattgc aaagaattat
     9541 agaagggatc ccacctttta ttttcttctt ttttccatat ttagggttga cagtgaaatc
     9601 agactggcaa cctattaatt gcttccacaa tgggacgaac ttgaagggga tgtcgtcgat
     9661 gatattatag gtggcgtgtt catcgtagtt ggtgaaatcg atggtaccgt tccaatagtt
     9721 gtgtcgtccg agacttctag cccaggtggt ctttccggta cgagttggtc cgcagatgta
     9781 gaggctgggg tgtcggattc cattccttcc attgtccttg ttaaatcggc catccattca
     9841 aggtcagatt gagcttgttg gtatgagaca ggatgtatgt aagtataagc gtctatgctt
     9901 acatggtata gatgggtttc cctccaggag tgtagatctt cgtggcagcg aagatctgat
     9961 tctgtgaagg gcgacacata cggttcaggt tgtggaggga ataatttgtt ggctgaatat
    10021 tccagccatt gaagctttgt tgcccattca tgagggaatt cttccttgat catgtcaaga
    10081 tattcctcct tagacgttgc agtctggata atagttctcc atcgtgcgtc agatttgcga
    10141 ggagaaacct tatgatctcg gaaatctcct ctggttttaa tatctccgtc ctttgatatg
    10201 taatcaagga cttgtttaga gtttctagct ggctggatat tagggtgatt tccttcaaaa
    10261 tcgaaaaaag aaggatccct aatacaaggt tttttatcaa gctggagaag agcatgatag
    10321 tgggtagtgc catcttgatg aagctcagaa gcaacaccaa ggaagaaaat aagaaaaggt
    10381 gtgagtttct cccagagaaa ctggaataaa tcatctcttt gagatgagca cttgggatag
    10441 gtaaggaaaa catatttaga ttggagtctg aagttcttac tagcagaagg catgttgttg
    10501 tgactccgag gggttgcctc aaactctatc ttataaccgg cgtggaggca tggaggcagg
    10561 ggtattttgg tcattttaat agatagtgga aaatgacgtg gaatttactt aaagacgaag
    10621 tctttgcgac aagggggggc ccacgccgaa tttaatatta ccggcgtggc ccccccttat
    10681 cgcgagtgct ttagcacgag cggtccagat ttaaagtaga aaatttcccg cccactaggg
    10741 ttaaaggtgt tcacactata aaagcatata cgatgtgatg gtatttgatg gagcgtatat
    10801 tgtatcaggt atttccgttg gatacgaatt attcgtacga ccctcatagt ttaaactatc
    10861 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
    10921 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
    10981 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
    11041 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
    11101 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
    11161 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
    11221 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
    11281 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
    11341 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
    11401 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
    11461 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
    11521 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
    11581 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
    11641 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
    11701 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
    11761 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
    11821 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
    11881 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
    11941 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ggctcaaccg
    12001 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
    12061 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
    12121 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
    12181 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
    12241 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
    12301 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
    12361 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
    12421 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
    12481 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
    12541 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
    12601 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
    12661 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
    12721 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
    12781 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
    12841 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
    12901 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
    12961 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
    13021 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
    13081 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
    13141 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt
    13201 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca
    13261 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
    13321 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
    13381 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
    13441 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
    13501 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgt
//
