LOCUS       sequence-243245-       13472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, WUS2, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13472)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 13472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM135
FEATURES             Location/Qualifiers
     source          1..13472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             66..695
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1129..2196
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      2262..2456
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(2692..2714)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2800..2940
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2855..2874
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2955..2972)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3126..3714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3206..3225)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3801..4595)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5020..5044
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5971..7623
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(6037..6055)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      7631..7879
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        8369..8444
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        8547..8726
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        8579..8762
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    12214..12238
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg
       61 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
      121 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
      181 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
      241 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
      301 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
      361 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
      421 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
      481 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
      541 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
      601 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
      661 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
      721 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac
      781 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga
      841 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct
      901 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa
      961 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg
     1021 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc
     1081 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca
     1141 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     1201 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     1261 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     1321 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     1381 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     1441 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     1501 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     1561 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     1621 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     1681 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     1741 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     1801 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     1861 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     1921 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     1981 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     2041 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     2101 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     2161 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     2221 attgccctag caggggaaaa aggtcgaaaa ggcctctttc ctgtggatag cacgtacatt
     2281 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     2341 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     2401 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     2461 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     2521 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     2581 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     2641 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     2701 catgcagctc ccggaaacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     2761 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     2821 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     2881 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     2941 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3001 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3061 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3541 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3721 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3781 ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc
     3841 tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg
     3901 atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg
     3961 cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct
     4021 cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca
     4081 tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg
     4141 gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta
     4201 tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg
     4261 ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc
     4321 tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca
     4381 ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc
     4441 cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta
     4501 tataccttag caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt
     4561 tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac
     4621 agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc
     4681 ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg
     4741 cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc
     4801 tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg
     4861 cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac
     4921 aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt
     4981 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
     5041 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtagagctc
     5101 aaagtttaac gcgttagcag aaggcatgtt gttgtgactc cgaggggttg cctcaaactc
     5161 tatcttataa ccggcgtgga ggcatggagg caggggtatt ttggtcattt taatagatag
     5221 tggaaaatga cgtggaattt acttaaagac gaagtctttg cgacaagggg gggcccacgc
     5281 cgaatttaat attaccggcg tggccccccc ttatcgcgag tgctttagca cgagcggtcc
     5341 agatttaaag tagaaaattt cccgcccact agggttaaag gtgttcacac tataaaagca
     5401 tatacgatgt gatggtattt gatggagcgt atattgtatc aggtatttcc gttggatacg
     5461 aattattcgt acgaccctcg gtaccgatcg gcgcgcctgg cagacatact gtcccacaaa
     5521 tgaagatgga atctgtaaaa gaaaacgcgt gaaataatgc gtctgacaaa ggttaggtcg
     5581 gctgccttta atcaatacca aagtggtccc taccacgatg gaaaaactgt gcagtcggtt
     5641 tggctttttc tgacgaacaa ataagattcg tggccgacag gtgggggtcc accatgtgaa
     5701 ggcatcttca gactccaata atggagcaat gacgtaaggg cttacgaaat aagtaagggt
     5761 agtttgggaa atgtccactc acccgtcagt ctataaatac ttagcccctc cctcattgtt
     5821 aagggagcaa aatctcagag agatagtcct agagagagaa agagagcaag tagcctagaa
     5881 gtagtcaagg cggcgaagta ttcaggcacg tggccaggaa gaagaaaagc caagacgacg
     5941 aaaacaggta agagctaagc ttcctgcacc atggaagacg ccaaaaacat aaagaaaggc
     6001 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
     6061 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
     6121 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
     6181 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
     6241 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     6301 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
     6361 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
     6421 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     6481 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
     6541 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg
     6601 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa
     6661 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     6721 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     6781 gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc
     6841 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     6901 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg
     6961 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg
     7021 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     7081 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg
     7141 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
     7201 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
     7261 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct
     7321 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc tggtgtcgca
     7381 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
     7441 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
     7501 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
     7561 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg
     7621 taactcgaga tatgaagatg aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg
     7681 cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt tttctaatat
     7741 taaatgaatg taagatcaca ttataatgaa taaacaaatg tttctataat ccattgtgaa
     7801 tgttttgttg gatctcttct gcagcatata actactgtat gtgctatggt atggactatg
     7861 gaatatgatt aaagataagg agctccggtg acggacccat ggcttcgttg aacaacggaa
     7921 actcgacttg ccttccgcac aatacatcat ttcttcttag ctttttttct tcttcttcgt
     7981 tcatacagtt tttttttgtt tatcagctta cattttcttg aaccgtagct ttcgttttct
     8041 tctttttaac tttccattcg gagtttttgt atcttgtttc atagtttgtc ccaggattag
     8101 aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca ttcttaagat
     8161 atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag gcccatttat
     8221 atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac aatcttcaaa
     8281 agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta gagtcgaagt
     8341 agtgattgtt ggtagtagcg actccatggt tttagagcta gaaatagcaa gttaaaataa
     8401 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tcccgggcgc
     8461 gccgatcatg agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac
     8521 aagccgtttt acgtttggaa ctgacagaac cgcaacgttg aaggagccac tcagccgcgg
     8581 gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag
     8641 tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc
     8701 cataaattcc cctcggtatc caattagagt ctcatattca ctctcaatcc aaataatctg
     8761 caccgtacct gcagggtccg agctaggtca cagaagcgct caggaaggcc gctgagatag
     8821 aggcatggcg gccaatgcgg gcggcggtgg agcgggagga ggcagcggca gcggcagcgt
     8881 ggctgcgccg gcggtgtgcc gccccagcgg ctcgcggtgg acgccgacgc cggagcagat
     8941 caggatgctg aaggagctgt actacggctg cggcatccgg tcgcccagct cggagcagat
     9001 ccagcgcatc accgccatgc tgcggcagca cggcaagatc gagggcaaga acgtcttcta
     9061 ctggttccag aaccacaagg cccgcgagcg ccagaagcgc cgcctcacca gcctcgacgt
     9121 gaacgtgccc gccgccggcg cggccgacgc caccaccagc caactcggcg tcctctcgct
     9181 gtcgtcgccg ccgccttcag gcgcggcgcc tccctcgccc accctcggct tctacgccgc
     9241 cggcaatggc ggcggatcgg ctgtgctgct ggacacgagt tccgactggg gcagcagcgg
     9301 cgctgcgatg gccaccgaga catgcttcct ccaggactac atgggcgtga cggacacggg
     9361 cagctcgtcg cagtggccac gcttctcgtc gtcggacacg ataatggcgg cggccgcggc
     9421 gcgggcggcg acgacgcggg cgcccgagac tctccctctc ttcccgacct gcggcgacga
     9481 cggcggcagc ggtagcagca gctacttgcc gttctggggt gccgcgtcca caactgccgg
     9541 cgccacttct tccgttgcga tccagcagca acaccagctg caggagcagt acagctttta
     9601 cagcaacagc aacagcaccc agctggccgg caccggcaac caagacgtat cggcaacagc
     9661 agcagcagcc gccgccctgg agctgagcct cagctcatgg tgctcccctt accctgctgc
     9721 agggagtatg tgagagcaac gcgagctgcc actgctcttc acttatgtct ctggaatgga
     9781 aggaggagga agtgagcata gcgttggtgc gttgctgtca ttgtcctagg ttagtagcta
     9841 gtgccagtta ctagtaagca tcaggcatag gagtatgtag tagaagcatg cacgttgccg
     9901 gccagccagg ctttagacgg gaaaagaatt tggtgcagcc ggctgcaaaa caggatgttt
     9961 acagcccccc cctcgagccc tagacttgtc catcttctgg attggccaag ttaattaatg
    10021 tatgaaataa aaggatgcac acatagtgac atgctaatca ctataatgtg ggcatcaaag
    10081 ttgtgtgtta tgtgtaatta ctaattatct gaataagaga aagagatcat ccatatttct
    10141 tatcctaaat gaatgtcacg tgtctttata attctttgat gaaccagatg cattttatta
    10201 accaattcca tatacatata aatattaatc atatataatt aatatcaatt gggttagcaa
    10261 aacaaatcta gtctaggtgt gttttgctaa ttattggggg atagtgcaaa aagaaatcta
    10321 cgttctcaat aattcagata gaaaacttaa taaagtgaga taatttacat agattgcttt
    10381 tatcctttga tatatgtgaa accatgcatg atataaggaa aatagataga gaaataattt
    10441 tttacatcgt tgaatatgta aacaatttaa ttcaagaagc taggaatata aatattgagg
    10501 agtttatgat tagagctctc ccggcgcgcc gatatcgagc tcagtgtttg atcgccggcg
    10561 gtaccgagtg tacttcaagt cagtgggaaa tcaataaaat gattatttta tgaatatatt
    10621 tcattgtgca agtagataga aattacatat gttacataac acacgaaata aacaaaaaaa
    10681 gacaatccaa aaacaaacac cccaaaaaaa ataatcactt tagataaact cgtatgagga
    10741 gaggcacgtt cagtgactcg acgattcccg agcaaaaaaa gtctccccgt cacacatgta
    10801 gtgggtgacg caattatctt taaagtaatc cttctgttga cttgtcattg ataacatcca
    10861 gtcttcgtca ggattgcaaa gaattataga agggatccca ccttttattt tcttcttttt
    10921 tccatattta gggttgacag tgaaatcaga ctggcaacct attaattgct tccacaatgg
    10981 gacgaacttg aaggggatgt cgtcgatgat attataggtg gcgtgttcat cgtagttggt
    11041 gaaatcgatg gtaccgttcc aatagttgtg tcgtccgaga cttctagccc aggtggtctt
    11101 tccggtacga gttggtccgc agatgtagag gctggggtgt cggattccat tccttccatt
    11161 gtccttgtta aatcggccat ccattcaagg tcagattgag cttgttggta tgagacagga
    11221 tgtatgtaag tataagcgtc tatgcttaca tggtatagat gggtttccct ccaggagtgt
    11281 agatcttcgt ggcagcgaag atctgattct gtgaagggcg acacatacgg ttcaggttgt
    11341 ggagggaata atttgttggc tgaatattcc agccattgaa gctttgttgc ccattcatga
    11401 gggaattctt ccttgatcat gtcaagatat tcctccttag acgttgcagt ctggataata
    11461 gttctccatc gtgcgtcaga tttgcgagga gaaaccttat gatctcggaa atctcctctg
    11521 gttttaatat ctccgtcctt tgatatgtaa tcaaggactt gtttagagtt tctagctggc
    11581 tggatattag ggtgatttcc ttcaaaatcg aaaaaagaag gatccctaat acaaggtttt
    11641 ttatcaagct ggagaagagc atgatagtgg gtagtgccat cttgatgaag ctcagaagca
    11701 acaccaagga agaaaataag aaaaggtgtg agtttctccc agagaaactg gaataaatca
    11761 tctctttgag atgagcactt gggataggta aggaaaacat atttagattg gagtctgaag
    11821 ttcttactag cagaaggcat gttgttgtga ctccgagggg ttgcctcaaa ctctatctta
    11881 taaccggcgt ggaggcatgg aggcaggggt attttggtca ttttaataga tagtggaaaa
    11941 tgacgtggaa tttacttaaa gacgaagtct ttgcgacaag ggggggccca cgccgaattt
    12001 aatattaccg gcgtggcccc cccttatcgc gagtgcttta gcacgagcgg tccagattta
    12061 aagtagaaaa tttcccgccc actagggtta aaggtgttca cactataaaa gcatatacga
    12121 tgtgatggta tttgatggag cgtatattgt atcaggtatt tccgttggat acgaattatt
    12181 cgtacgaccc tcatagttta aactatcagt gtttgacagg atatattggc gggtaaacct
    12241 aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc
    12301 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
    12361 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
    12421 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
    12481 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
    12541 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
    12601 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
    12661 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
    12721 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
    12781 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
    12841 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
    12901 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
    12961 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
    13021 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
    13081 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
    13141 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
    13201 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
    13261 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
    13321 tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
    13381 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
    13441 taaaaaggtg atgtgtattt gagtaaaaca gc
//
