LOCUS       sequence-243246-       14829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14829)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 14829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM136
FEATURES             Location/Qualifiers
     source          1..14829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      960..1154
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1390..1412)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1498..1638
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1553..1572
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1653..1670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1824..2412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1904..1923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2499..3293)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3718..3742
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4669..6321
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4735..4753)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      6329..6577
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        7067..7142
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7668..8013
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7956..7976
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    10413..10587
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12269..12293
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             13593..14222
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(14656..14829,1..894)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
ORIGIN
        1 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
       61 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
      121 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
      181 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
      241 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
      301 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
      361 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
      421 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
      481 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
      541 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
      601 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
      661 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
      721 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
      781 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
      841 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
      901 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg cctctttcct
      961 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     1021 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     1081 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     1141 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     1201 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     1261 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     1321 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
     1381 cggtgaaaac ctctgacaca tgcagctccc ggaaacggtc acagcttgtc tgtaagcgga
     1441 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     1501 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     1561 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     1621 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1681 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1741 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1801 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1861 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1981 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2221 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     2281 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2341 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2401 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2461 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
     2521 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     2581 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     2641 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     2701 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     2761 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     2821 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
     2881 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
     2941 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
     3001 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
     3061 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
     3121 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
     3181 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
     3241 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
     3301 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     3361 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     3421 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
     3481 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
     3541 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
     3601 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
     3661 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     3721 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
     3781 gtttttaatg tagagctcaa agtttaacgc gttagcagaa ggcatgttgt tgtgactccg
     3841 aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca ggggtatttt
     3901 ggtcatttta atagatagtg gaaaatgacg tggaatttac ttaaagacga agtctttgcg
     3961 acaagggggg gcccacgccg aatttaatat taccggcgtg gccccccctt atcgcgagtg
     4021 ctttagcacg agcggtccag atttaaagta gaaaatttcc cgcccactag ggttaaaggt
     4081 gttcacacta taaaagcata tacgatgtga tggtatttga tggagcgtat attgtatcag
     4141 gtatttccgt tggatacgaa ttattcgtac gaccctcggt accgatcggc gcgcctggca
     4201 gacatactgt cccacaaatg aagatggaat ctgtaaaaga aaacgcgtga aataatgcgt
     4261 ctgacaaagg ttaggtcggc tgcctttaat caataccaaa gtggtcccta ccacgatgga
     4321 aaaactgtgc agtcggtttg gctttttctg acgaacaaat aagattcgtg gccgacaggt
     4381 gggggtccac catgtgaagg catcttcaga ctccaataat ggagcaatga cgtaagggct
     4441 tacgaaataa gtaagggtag tttgggaaat gtccactcac ccgtcagtct ataaatactt
     4501 agcccctccc tcattgttaa gggagcaaaa tctcagagag atagtcctag agagagaaag
     4561 agagcaagta gcctagaagt agtcaaggcg gcgaagtatt caggcacgtg gccaggaaga
     4621 agaaaagcca agacgacgaa aacaggtaag agctaagctt cctgcaccat ggaagacgcc
     4681 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
     4741 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     4801 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
     4861 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     4921 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     4981 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
     5041 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
     5101 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     5161 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
     5221 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     5281 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     5341 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     5401 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     5461 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     5521 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     5581 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     5641 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     5701 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     5761 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     5821 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     5881 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     5941 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     6001 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     6061 ttcgacgctg gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     6121 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     6181 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     6241 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     6301 ggcggaaaga tcgccgtgta actcgagata tgaagatgaa gatgaaatat ttggtgtgtc
     6361 aaataaaaag cttgtgtgct taagtttgtg tttttttctt ggcttgttgt gttatgaatt
     6421 tgtggctttt tctaatatta aatgaatgta agatcacatt ataatgaata aacaaatgtt
     6481 tctataatcc attgtgaatg ttttgttgga tctcttctgc agcatataac tactgtatgt
     6541 gctatggtat ggactatgga atatgattaa agataaggag ctccggtgac ggacccatgg
     6601 cttcgttgaa caacggaaac tcgacttgcc ttccgcacaa tacatcattt cttcttagct
     6661 ttttttcttc ttcttcgttc atacagtttt tttttgttta tcagcttaca ttttcttgaa
     6721 ccgtagcttt cgttttcttc tttttaactt tccattcgga gtttttgtat cttgtttcat
     6781 agtttgtccc aggattagaa tgattaggca tcgaaccttc aagaatttga ttgaataaaa
     6841 catcttcatt cttaagatat gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag
     6901 agaagcaggc ccatttatat gggaaagaac aatagtattt cttatatagg cccatttaag
     6961 ttgaaaacaa tcttcaaaag tcccacatcg cttagataag aaaacgaagc tgagtttata
     7021 tacagctaga gtcgaagtag tgattgttgg tagtagcgac tccatggttt tagagctaga
     7081 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     7141 gctttttttc ccggggcgcg ccgatatcga gctctcccgc agatttgcct tttcaatttc
     7201 agaaagaatg ctaacccaca gatggttaga gaggcttacg cagcaggtat catcaagacg
     7261 atctacccga gcaataatct ccaggaaatc aaataccttc ccaagaaggt taaagatgca
     7321 gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc
     7381 agaagtacta ttccagtatg gacgattcaa ggcttgcttc acaaaccaag gcaagtaata
     7441 gagattggag tctctaaaaa ggtagttccc actgaatcaa aggccatgga gtcaaagatt
     7501 caaatagagg acctaacaga actcgccgta aagactggcg aacagttcat acagagtctc
     7561 ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cacacttgtc
     7621 tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa
     7681 caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt
     7741 gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag
     7801 gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
     7861 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
     7921 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
     7981 atataaggaa gttcatttca tttggagaga acacggggga ctatgatggc ttcattgtct
     8041 tgtgttgaag acaagatgaa aacaagttgt ttggttaatg gtggaggaac tataacaaca
     8101 acaacatctc aatctacctt gcttgaagag atgaagctgt tgaaagacca gtcaggtaca
     8161 agaaagccgg taataaactc ggagctatgg cacgcttgtg caggcccttt ggtgtgtctc
     8221 cctcaagttg ggagcttagt gtattacttc tcacaaggtc atagcgagca ggttgctgtt
     8281 tcaaccagaa gatcagcaac aacacaagtt cctaattatc cgaaccttcc atctcagttg
     8341 atgtgtcaag tccataatgt tactcttcat gctgacaaag acagtgacga aatctatgct
     8401 cagatgagtc ttcaacctgt tcactctgag agagatgtgt tccctgtacc agactttgga
     8461 atgctgagag gaagtaagca cccgactgag tttttctgca aaacacttac tgcaagtgac
     8521 acaagcacac atggaggttt ctcagtgcca cgtagagctg cagagaagct atttccacca
     8581 ttggactact cagcacagcc gccaacgcaa gagcttgtag ttcgagatct tcatgagaat
     8641 acttggacat ttcgccatat ctaccgaggg caaccaaaga gacatctcct aactacagga
     8701 tggagtttgt tcgttggatc gaagagattg agagctgggg attctgtttt gttcatcagg
     8761 gatgagaagt cacaacttat ggtcggtgtt aggcgtgcca atcgccaaca aacagcactt
     8821 ccttcatcag ttctctcagc ggatagtatg cacatcggtg ttcttgctgc tgctgctcac
     8881 gcaaccgcca accgtactcc ttttttgata ttctataatc caagagcttg tccagcagag
     8941 ttcgtgatcc ctctagctaa gtaccgtaag gcgatatgcg ggtctcagct ctcagttggt
     9001 atgagatttg gaatgatgtt tgaaactgaa gattccggga aacgaaggta catgggaact
     9061 attgttggaa tcagcgattt ggatccgttg agatggcctg gttctaagtg gcgtaacctt
     9121 caggtagaat gggatgagcc tggatgtaat gataaaccta ctcgggtcag tccatgggat
     9181 atcgaaacac ctgaaagtct cttcattttt ccttctctga cctcaggact caaacgtcag
     9241 ctccatccat cttactttgc tggtgaaact gaatggggta gcttgataaa acggccactt
     9301 atacgtgttc ctgattccgc gaatgggatt atgccatatg catctttccc tagtatggct
     9361 tcggagcagc ttatgaaaat gatgatgagg cctcacaaca accaaaatgt accatctttc
     9421 atgtctgaga tgcagcagaa tattgtaatg gggaatggag gtttgctagg agatatgaag
     9481 atgcagcaac ccctgatgat gaaccagaaa tctgagatgg tgcagccaca aaacaagcta
     9541 acagtgaacc catctgcttc taatacgagt ggccaagaac agaatctttc acagagtatg
     9601 agtgctcctg ctaaacctga gaactctaca ctctctggtt gcagctctgg tagagtccaa
     9661 catggacttg agcagtcaat ggaacaggca agccaggtta ctacatccac agtgtgtaat
     9721 gaggaaaagg ttaatcagct acttcagaaa ccgggtgctt cgtcgcctgt acaagctgat
     9781 caatgtcttg acattactca tcagatttac caaccacagt ctgatccaat aaatggattc
     9841 tctttcctgg aaactgatga gctgacatca caagtctctt ccttccagtc tcttgccgga
     9901 tcatacaagc aaccattcat tctatcctcc caggattctt cagctgttgt gttaccggat
     9961 tccacaaact caccgctgtt tcatgatgtg tgggacactc agttgaacgg tctcaagttt
    10021 gaccagttca gtcccttgat gcagcaggac ctttatgcta gtcagaatat ctgtatgagt
    10081 aatagcacaa ccagtaacat tctagatcct ccactctcaa acacagtcct tgatgacttc
    10141 tgtgccatca aagacactga tttccagaac cacccttctg gttgtttggt tggaaacaac
    10201 aacactagct ttgctcaaga tgtccagtcg cagatcacat cagctagctt tgcagactca
    10261 caggccttct ctcgccaaga ttttccagat aattctggag gcactggtac atcttcaagc
    10321 aatgttgatt ttgatgattg tagtctgcgg caaaatagta aaggctcatc atggcagaaa
    10381 attgcgacac cccgcgtccg aacctactcg agtttctcca taataatgtg tgagtagttc
    10441 ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac
    10501 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
    10561 ccaaaatcca gtactaaaat ccagatcccc cgaattaagt gtttgatcgc cggcggtacc
    10621 gagtgtactt caagtcagtg ggaaatcaat aaaatgatta ttttatgaat atatttcatt
    10681 gtgcaagtag atagaaatta catatgttac ataacacacg aaataaacaa aaaaagacaa
    10741 tccaaaaaca aacaccccaa aaaaaataat cactttagat aaactcgtat gaggagaggc
    10801 acgttcagtg actcgacgat tcccgagcaa aaaaagtctc cccgtcacac atgtagtggg
    10861 tgacgcaatt atctttaaag taatccttct gttgacttgt cattgataac atccagtctt
    10921 cgtcaggatt gcaaagaatt atagaaggga tcccaccttt tattttcttc ttttttccat
    10981 atttagggtt gacagtgaaa tcagactggc aacctattaa ttgcttccac aatgggacga
    11041 acttgaaggg gatgtcgtcg atgatattat aggtggcgtg ttcatcgtag ttggtgaaat
    11101 cgatggtacc gttccaatag ttgtgtcgtc cgagacttct agcccaggtg gtctttccgg
    11161 tacgagttgg tccgcagatg tagaggctgg ggtgtcggat tccattcctt ccattgtcct
    11221 tgttaaatcg gccatccatt caaggtcaga ttgagcttgt tggtatgaga caggatgtat
    11281 gtaagtataa gcgtctatgc ttacatggta tagatgggtt tccctccagg agtgtagatc
    11341 ttcgtggcag cgaagatctg attctgtgaa gggcgacaca tacggttcag gttgtggagg
    11401 gaataatttg ttggctgaat attccagcca ttgaagcttt gttgcccatt catgagggaa
    11461 ttcttccttg atcatgtcaa gatattcctc cttagacgtt gcagtctgga taatagttct
    11521 ccatcgtgcg tcagatttgc gaggagaaac cttatgatct cggaaatctc ctctggtttt
    11581 aatatctccg tcctttgata tgtaatcaag gacttgttta gagtttctag ctggctggat
    11641 attagggtga tttccttcaa aatcgaaaaa agaaggatcc ctaatacaag gttttttatc
    11701 aagctggaga agagcatgat agtgggtagt gccatcttga tgaagctcag aagcaacacc
    11761 aaggaagaaa ataagaaaag gtgtgagttt ctcccagaga aactggaata aatcatctct
    11821 ttgagatgag cacttgggat aggtaaggaa aacatattta gattggagtc tgaagttctt
    11881 actagcagaa ggcatgttgt tgtgactccg aggggttgcc tcaaactcta tcttataacc
    11941 ggcgtggagg catggaggca ggggtatttt ggtcatttta atagatagtg gaaaatgacg
    12001 tggaatttac ttaaagacga agtctttgcg acaagggggg gcccacgccg aatttaatat
    12061 taccggcgtg gccccccctt atcgcgagtg ctttagcacg agcggtccag atttaaagta
    12121 gaaaatttcc cgcccactag ggttaaaggt gttcacacta taaaagcata tacgatgtga
    12181 tggtatttga tggagcgtat attgtatcag gtatttccgt tggatacgaa ttattcgtac
    12241 gaccctcata gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
    12301 aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg
    12361 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
    12421 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
    12481 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
    12541 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
    12601 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
    12661 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
    12721 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
    12781 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
    12841 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
    12901 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
    12961 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
    13021 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
    13081 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
    13141 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
    13201 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
    13261 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
    13321 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
    13381 ccgcccgcgc acggctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
    13441 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
    13501 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
    13561 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
    13621 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
    13681 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
    13741 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
    13801 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
    13861 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
    13921 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
    13981 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
    14041 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
    14101 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
    14161 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
    14221 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
    14281 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
    14341 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
    14401 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
    14461 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
    14521 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
    14581 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg
    14641 aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
    14701 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca
    14761 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
    14821 caaccgccg
//
