LOCUS       sequence-243247-       16222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, BBM, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16222)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 16222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM146
FEATURES             Location/Qualifiers
     source          1..16222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      747..941
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1177..1199)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1285..1425
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1340..1359
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1440..1457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1611..2199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1691..1710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2286..3080)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3505..3529
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4456..6108
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4522..4540)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      6116..6364
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        6854..6929
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     RBS             10040..10051
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    13449..13473
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14773..15402
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(15836..16222,1..681)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
ORIGIN
        1 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
       61 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
      121 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
      181 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
      241 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
      301 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
      361 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
      421 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
      481 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
      541 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
      601 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
      661 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
      721 gaaaaaggtc gaaaaggcct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
      781 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
      841 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
      901 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
      961 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     1021 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     1081 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     1141 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     1201 aacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     1261 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     1321 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     1381 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     1441 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1501 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1561 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1621 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1681 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1741 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1801 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1861 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1921 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1981 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2041 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2101 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2161 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2221 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
     2281 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     2341 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     2401 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     2461 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     2521 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     2581 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     2641 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     2701 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     2761 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     2821 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     2881 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     2941 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     3001 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     3061 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     3121 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     3181 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     3241 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
     3301 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     3361 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     3421 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     3481 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     3541 tagacaactt aataacacat tgcggacgtt tttaatgtag agctcaaagt ttaacgcgtt
     3601 agcagaaggc atgttgttgt gactccgagg ggttgcctca aactctatct tataaccggc
     3661 gtggaggcat ggaggcaggg gtattttggt cattttaata gatagtggaa aatgacgtgg
     3721 aatttactta aagacgaagt ctttgcgaca agggggggcc cacgccgaat ttaatattac
     3781 cggcgtggcc cccccttatc gcgagtgctt tagcacgagc ggtccagatt taaagtagaa
     3841 aatttcccgc ccactagggt taaaggtgtt cacactataa aagcatatac gatgtgatgg
     3901 tatttgatgg agcgtatatt gtatcaggta tttccgttgg atacgaatta ttcgtacgac
     3961 cctcggtacc gatcggcgcg cctggcagac atactgtccc acaaatgaag atggaatctg
     4021 taaaagaaaa cgcgtgaaat aatgcgtctg acaaaggtta ggtcggctgc ctttaatcaa
     4081 taccaaagtg gtccctacca cgatggaaaa actgtgcagt cggtttggct ttttctgacg
     4141 aacaaataag attcgtggcc gacaggtggg ggtccaccat gtgaaggcat cttcagactc
     4201 caataatgga gcaatgacgt aagggcttac gaaataagta agggtagttt gggaaatgtc
     4261 cactcacccg tcagtctata aatacttagc ccctccctca ttgttaaggg agcaaaatct
     4321 cagagagata gtcctagaga gagaaagaga gcaagtagcc tagaagtagt caaggcggcg
     4381 aagtattcag gcacgtggcc aggaagaaga aaagccaaga cgacgaaaac aggtaagagc
     4441 taagcttcct gcaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     4501 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     4561 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
     4621 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     4681 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     4741 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     4801 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
     4861 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
     4921 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     4981 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
     5041 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
     5101 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     5161 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     5221 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
     5281 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
     5341 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     5401 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     5461 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     5521 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     5581 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     5641 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     5701 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
     5761 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     5821 tccatcttgc tccaacaccc caacatcttc gacgctggtg tcgcaggtct tcccgacgat
     5881 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     5941 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     6001 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     6061 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaact cgagatatga
     6121 agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt
     6181 ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga
     6241 tcacattata atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct
     6301 cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga
     6361 taaggagctc cggtgacgga cccatggctt cgttgaacaa cggaaactcg acttgccttc
     6421 cgcacaatac atcatttctt cttagctttt tttcttcttc ttcgttcata cagttttttt
     6481 ttgtttatca gcttacattt tcttgaaccg tagctttcgt tttcttcttt ttaactttcc
     6541 attcggagtt tttgtatctt gtttcatagt ttgtcccagg attagaatga ttaggcatcg
     6601 aaccttcaag aatttgattg aataaaacat cttcattctt aagatatgaa gataatcttc
     6661 aaaaggcccc tgggaatctg aaagaagaga agcaggccca tttatatggg aaagaacaat
     6721 agtatttctt atataggccc atttaagttg aaaacaatct tcaaaagtcc cacatcgctt
     6781 agataagaaa acgaagctga gtttatatac agctagagtc gaagtagtga ttgttggtag
     6841 tagcgactcc atggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     6901 aacttgaaaa agtggcaccg agtcggtgct ttttttcccg gggcgcgccg atatcgagct
     6961 ctcccggcgc gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc
     7021 attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt
     7081 gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata
     7141 gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta
     7201 aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt
     7261 gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta
     7321 catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt
     7381 ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta
     7441 ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta
     7501 agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt
     7561 aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc
     7621 aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc gagagttccg
     7681 ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac
     7741 gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc tacgggggat
     7801 tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc
     7861 tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct
     7921 cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc
     7981 ccctctctac cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact
     8041 tctgttcatg tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac
     8101 acggatgcga cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg
     8161 gggaatcctg ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg
     8221 tttcgttgca tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg
     8281 tttgtcgggt catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg
     8341 ggcggtcgtt ctagatcgga gtagaattaa ttctgtttca aactacctgg tggatttatt
     8401 aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga
     8461 tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca
     8521 gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg
     8581 ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact
     8641 gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct
     8701 aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag
     8761 catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta
     8821 taattatttt gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt
     8881 ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc
     8941 accctgttgt ttggtgttac ttcctgcagg cttccctaac ctttgcactg tccaaaatgg
     9001 cttcctgatc ccctcacttc ctcgaatcaa tctaagaaga aactcaagcc gcaaccatta
     9061 ggggcagatt aattgctgca ctttcagata atcaagcatg gccactgtga acaactggct
     9121 cgctttctcc ctctccccgc aggagctgcc gccctcccag acgacggact ccacactcat
     9181 ctcggccgcc accgccgacc atgtctccgg cgatgtctgc ttcaacatcc cccaagattg
     9241 gagcatgagg ggatcagagc tttcggcgct cgtcgcggag ccgaagctgg aggacttcct
     9301 cggcggcatc tccttctccg agcagcatca caaggccaac tgcaacatga tacccagcac
     9361 tagcagcaca gtttgctacg ccagctcagg tgctagcacc ggctaccatc accagctgta
     9421 ccaccagccc accagctcag cgctccactt cgcggactcc gtaatggtgg cctcctcggc
     9481 cggtgtccac gacggcggtg ccatgctcag cgcggccgcc gctaacggtg tcgctggcgc
     9541 tgccagtgcc aacggcggcg gcatcgggct gtccatgatt aagaactggc tgcggagcca
     9601 accggcgccc atgcagccga gggtggcggc ggctgagggc gcgcaggggc tctctttgtc
     9661 catgaacatg gcggggacga cccaaggcgc tgctggcatg ccacttctcg ctggagagcg
     9721 cgcacgggcg cccgagagtg tatccacgtc agcacagggt ggagccgtcg tcgtcacggc
     9781 gccgaaggag gatagcggtg gcagcggtgt tgccggcgct ctagtagccg tgagcacgga
     9841 cacgggtggc agcggcggcg cgtcggctga caacacggca aggaagacgg tggacacgtt
     9901 cgggcagcgc acgtcgattt accgtggcgt gacaaggcat agatggactg ggagatatga
     9961 ggcacatctt tgggataaca gttgcagaag ggaagggcaa actcgtaagg gtcgtcaagt
    10021 ctatttaggt ggctatgata aagaggagaa agctgctagg gcttatgatc ttgctgctct
    10081 gaagtactgg ggtgccacaa caacaacaaa ttttccagtg agtaactacg aaaaggagct
    10141 ggaggacatg aagcacatga caaggcagga gtttgtagcg cctctgagaa ggaagtccag
    10201 tggtttctcc agaggtgcat ccatttacag gggagtgact aggcatcacc aacatggaag
    10261 atggcaagca cggattggac gagttgcagg gaacaaggat ctttacttgg gcaccttcag
    10321 cacccaggag gaggcagcgg aggcgtacga catcgcggcg atcaagttcc gcggcctcaa
    10381 cgccgtcacc aacttcgaca tgagccgcta cgacgtgaag tccatcctgg acagcagcgc
    10441 cctccccatc ggcagcgccg ccaagcgcct caaggaggcc gaggccgcag cgtccgcgca
    10501 gcaccaccat gcgggtgtcg tttcctatga cgttgggagg attgccagcc aactgggaga
    10561 tggcggtgcc ctcgctgcgg cctatggtgc tcactatcac ggtgccgcgt ggccaacgat
    10621 tgcattccag ccgggcgcgg cgtccaccgg actgtaccat ccttacgcgc agcagcctat
    10681 gcgcggcggt ggatggtgta aacaagagca agatcacgct gtgatagcag cggcacactc
    10741 cttgcaggat cttcatcatt tgaatctcgg agccgccggg gcccacgact ttttctcggc
    10801 agggcagcag gccgccgccg ctgcgatgca cggcctgggt agcatcgaca gtgcgtcgct
    10861 ggagcacagc accggctcca actccgtcgt ctacaacggc ggggtcggcg acagcaacgg
    10921 cgccagcgcc gtcggcggca gtggcggtgg ctacatgatg ccgatgagcg ctgccggagc
    10981 aaccactaca tcggcaatgg tgagccacga gcaggtccat gcacgggcct acgacgaagc
    11041 caagcaggct gctcagatgg ggtacgagag ctacctggtg aacgcggaga acaatggtgg
    11101 cggaaggatg tctgcatggg ggactgtcgt gtctgcagcc gcggcggcag cagcaagcag
    11161 caacgacaac atggccgccg acgtgggcca cggcggcgcg cagctgttca gtgtctggaa
    11221 cgacacttaa ctcgagccct agacttgtcc atcttctgga ttggccaagt taattaatgt
    11281 atgaaataaa aggatgcaca catagtgaca tgctaatcac tataatgtgg gcatcaaagt
    11341 tgtgtgttat gtgtaattac taattatctg aataagagaa agagatcatc catatttctt
    11401 atcctaaatg aatgtcacgt gtctttataa ttctttgatg aaccagatgc attttattaa
    11461 ccaattccat atacatataa atattaatca tatataatta atatcaattg ggttagcaaa
    11521 acaaatctag tctaggtgtg ttttgctaat tattggggga tagtgcaaaa agaaatctac
    11581 gttctcaata attcagatag aaaacttaat aaagtgagat aatttacata gattgctttt
    11641 atcctttgat atatgtgaaa ccatgcatga tataaggaaa atagatagag aaataatttt
    11701 ttacatcgtt gaatatgtaa acaatttaat tcaagaagct aggaatataa atattgagga
    11761 gtttatgatt agagctcagt gtttgatcgc cggcggtacc gagtgtactt caagtcagtg
    11821 ggaaatcaat aaaatgatta ttttatgaat atatttcatt gtgcaagtag atagaaatta
    11881 catatgttac ataacacacg aaataaacaa aaaaagacaa tccaaaaaca aacaccccaa
    11941 aaaaaataat cactttagat aaactcgtat gaggagaggc acgttcagtg actcgacgat
    12001 tcccgagcaa aaaaagtctc cccgtcacac atgtagtggg tgacgcaatt atctttaaag
    12061 taatccttct gttgacttgt cattgataac atccagtctt cgtcaggatt gcaaagaatt
    12121 atagaaggga tcccaccttt tattttcttc ttttttccat atttagggtt gacagtgaaa
    12181 tcagactggc aacctattaa ttgcttccac aatgggacga acttgaaggg gatgtcgtcg
    12241 atgatattat aggtggcgtg ttcatcgtag ttggtgaaat cgatggtacc gttccaatag
    12301 ttgtgtcgtc cgagacttct agcccaggtg gtctttccgg tacgagttgg tccgcagatg
    12361 tagaggctgg ggtgtcggat tccattcctt ccattgtcct tgttaaatcg gccatccatt
    12421 caaggtcaga ttgagcttgt tggtatgaga caggatgtat gtaagtataa gcgtctatgc
    12481 ttacatggta tagatgggtt tccctccagg agtgtagatc ttcgtggcag cgaagatctg
    12541 attctgtgaa gggcgacaca tacggttcag gttgtggagg gaataatttg ttggctgaat
    12601 attccagcca ttgaagcttt gttgcccatt catgagggaa ttcttccttg atcatgtcaa
    12661 gatattcctc cttagacgtt gcagtctgga taatagttct ccatcgtgcg tcagatttgc
    12721 gaggagaaac cttatgatct cggaaatctc ctctggtttt aatatctccg tcctttgata
    12781 tgtaatcaag gacttgttta gagtttctag ctggctggat attagggtga tttccttcaa
    12841 aatcgaaaaa agaaggatcc ctaatacaag gttttttatc aagctggaga agagcatgat
    12901 agtgggtagt gccatcttga tgaagctcag aagcaacacc aaggaagaaa ataagaaaag
    12961 gtgtgagttt ctcccagaga aactggaata aatcatctct ttgagatgag cacttgggat
    13021 aggtaaggaa aacatattta gattggagtc tgaagttctt actagcagaa ggcatgttgt
    13081 tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca
    13141 ggggtatttt ggtcatttta atagatagtg gaaaatgacg tggaatttac ttaaagacga
    13201 agtctttgcg acaagggggg gcccacgccg aatttaatat taccggcgtg gccccccctt
    13261 atcgcgagtg ctttagcacg agcggtccag atttaaagta gaaaatttcc cgcccactag
    13321 ggttaaaggt gttcacacta taaaagcata tacgatgtga tggtatttga tggagcgtat
    13381 attgtatcag gtatttccgt tggatacgaa ttattcgtac gaccctcata gtttaaacta
    13441 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
    13501 aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
    13561 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
    13621 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
    13681 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
    13741 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
    13801 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
    13861 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
    13921 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
    13981 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
    14041 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
    14101 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
    14161 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
    14221 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
    14281 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
    14341 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
    14401 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
    14461 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
    14521 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acggctcaac
    14581 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
    14641 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
    14701 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
    14761 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
    14821 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
    14881 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
    14941 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
    15001 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
    15061 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
    15121 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
    15181 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
    15241 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
    15301 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
    15361 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
    15421 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
    15481 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
    15541 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
    15601 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
    15661 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
    15721 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
    15781 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg
    15841 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
    15901 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
    15961 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
    16021 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
    16081 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
    16141 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
    16201 ggtttccgca gggccggccg gc
//
