LOCUS       sequence-243248-        4083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C' with Pnos::WUS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMOD_C'5014
FEATURES             Location/Qualifiers
     source          1..4083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        341..520
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        373..556
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2371..2475
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(3543..4083,1..48)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4032..4051
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      181 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccacct
      241 gcaacgccgg gcgcgccgat catgagcgga gaattaaggg agtcacgtta tgacccccgc
      301 cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag
      361 ccactcagcc gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat
      421 tgcgcgttca aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg
      481 ctccactgac gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca
      541 atccaaataa tctgcaccgt acctgcaggg tccgagctag gtcacagaag cgctcaggaa
      601 ggccgctgag atagaggcat ggcggccaat gcgggcggcg gtggagcggg aggaggcagc
      661 ggcagcggca gcgtggctgc gccggcggtg tgccgcccca gcggctcgcg gtggacgccg
      721 acgccggagc agatcaggat gctgaaggag ctgtactacg gctgcggcat ccggtcgccc
      781 agctcggagc agatccagcg catcaccgcc atgctgcggc agcacggcaa gatcgagggc
      841 aagaacgtct tctactggtt ccagaaccac aaggcccgcg agcgccagaa gcgccgcctc
      901 accagcctcg acgtgaacgt gcccgccgcc ggcgcggccg acgccaccac cagccaactc
      961 ggcgtcctct cgctgtcgtc gccgccgcct tcaggcgcgg cgcctccctc gcccaccctc
     1021 ggcttctacg ccgccggcaa tggcggcgga tcggctgtgc tgctggacac gagttccgac
     1081 tggggcagca gcggcgctgc gatggccacc gagacatgct tcctccagga ctacatgggc
     1141 gtgacggaca cgggcagctc gtcgcagtgg ccacgcttct cgtcgtcgga cacgataatg
     1201 gcggcggccg cggcgcgggc ggcgacgacg cgggcgcccg agactctccc tctcttcccg
     1261 acctgcggcg acgacggcgg cagcggtagc agcagctact tgccgttctg gggtgccgcg
     1321 tccacaactg ccggcgccac ttcttccgtt gcgatccagc agcaacacca gctgcaggag
     1381 cagtacagct tttacagcaa cagcaacagc acccagctgg ccggcaccgg caaccaagac
     1441 gtatcggcaa cagcagcagc agccgccgcc ctggagctga gcctcagctc atggtgctcc
     1501 ccttaccctg ctgcagggag tatgtgagag caacgcgagc tgccactgct cttcacttat
     1561 gtctctggaa tggaaggagg aggaagtgag catagcgttg gtgcgttgct gtcattgtcc
     1621 taggttagta gctagtgcca gttactagta agcatcaggc ataggagtat gtagtagaag
     1681 catgcacgtt gccggccagc caggctttag acgggaaaag aatttggtgc agccggctgc
     1741 aaaacaggat gtttacagcc cccccctcga gccctagact tgtccatctt ctggattggc
     1801 caagttaatt aatgtatgaa ataaaaggat gcacacatag tgacatgcta atcactataa
     1861 tgtgggcatc aaagttgtgt gttatgtgta attactaatt atctgaataa gagaaagaga
     1921 tcatccatat ttcttatcct aaatgaatgt cacgtgtctt tataattctt tgatgaacca
     1981 gatgcatttt attaaccaat tccatataca tataaatatt aatcatatat aattaatatc
     2041 aattgggtta gcaaaacaaa tctagtctag gtgtgttttg ctaattattg ggggatagtg
     2101 caaaaagaaa tctacgttct caataattca gatagaaaac ttaataaagt gagataattt
     2161 acatagattg cttttatcct ttgatatatg tgaaaccatg catgatataa ggaaaataga
     2221 tagagaaata attttttaca tcgttgaata tgtaaacaat ttaattcaag aagctaggaa
     2281 tataaatatt gaggagttta tgattagagc tctcccacta gcaggtgatg aaaagcctta
     2341 gggtcaggtg gcacttttcg gggaaatgtg cgatgaaccc ctatttgttt atttttctaa
     2401 atacattcaa atatgtatcc gctaatgaga caataaccct gataaatgct tcaattatat
     2461 tgaaaaagga agagtatgag tatccaacat ttccgtgtcg cccttattcc cttttttgcg
     2521 gccttctgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2581 gatcagttgg gtgcccgagt gggttacatt gaactggatc tcaacagcgg caaaatctta
     2641 gagagttttc gccccgaaga acgcttccca atgatgagca ctttcaaagt tctgctatgt
     2701 ggcgcggtgt tatcccgtat tgatgctggg caagagcaac tcggtcgccg catacactat
     2761 tctcagaatg acttggttga gtattcacca gtcacagaaa agcatcttac ggatggcatg
     2821 acagtaagag aattatgtag tgctgccata accatgagtg ataacactgc ggccaactta
     2881 cttctgacaa cgattggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2941 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3001 cgtgacacca cgatgcctgt agcaatggca acaactctcc gcaaactatt aactggcgaa
     3061 ctacttactc ttgcttcacg ccagcaactc attgactgga tggaggcgga caaagttgca
     3121 ggaccacttc tgcgctcggc acttccggct ggctggttta ttgctgataa atctggagcc
     3181 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3241 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3301 gctgagatag gtgcctcact gattaagcat tggtaactgt cagacgaagg gaaataaata
     3361 gtagcccgcc tgatgtgcgg gctttttttt ggtggtggtt accagtgtcg cttagctacc
     3421 gtaggtcctc ttaggcggtc cgattagcga catagtcatc aatgccttga cggcaaaatc
     3481 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3541 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3601 ccagcggtgg tttgtttgcc ggatcaagag ctactaactc tttttccgaa ggtaactggc
     3661 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3721 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3781 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3841 aaggcgcagc ggtcgggctg aacggggggt tcgtgctcac agcccagctt ggagcgaacg
     3901 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3961 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4021 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4081 ctt
//
