LOCUS       sequence-243254-        2577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AtU6::sgRNA targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM100
FEATURES             Location/Qualifiers
     source          1..2577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_RNA        1343..1418
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1485..1589
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gccaggggac ccatggcttc gttgaacaac ggaaactcga
      901 cttgccttcc gcacaataca tcatttcttc ttagcttttt ttcttcttct tcgttcatac
      961 agtttttttt tgtttatcag cttacatttt cttgaaccgt agctttcgtt ttcttctttt
     1021 taactttcca ttcggagttt ttgtatcttg tttcatagtt tgtcccagga ttagaatgat
     1081 taggcatcga accttcaaga atttgattga ataaaacatc ttcattctta agatatgaag
     1141 ataatcttca aaaggcccct gggaatctga aagaagagaa gcaggcccat ttatatggga
     1201 aagaacaata gtatttctta tataggccca tttaagttga aaacaatctt caaaagtccc
     1261 acatcgctta gataagaaaa cgaagctgag tttatataca gctagagtcg aagtagtgat
     1321 tgttggtagt agcgactcca tggttttaga gctagaaata gcaagttaaa ataaggctag
     1381 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttcccgg tgacgcaggt
     1441 gatgaaaagc cttagggtca ggtggcactt ttcggggaaa tgtgcgatga acccctattt
     1501 gtttattttt ctaaatacat tcaaatatgt atccgctaat gagacaataa ccctgataaa
     1561 tgcttcaatt atattgaaaa aggaagagta tgagtatcca acatttccgt gtcgccctta
     1621 ttcccttttt tgcggccttc tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1681 taaaagatgc tgaagatcag ttgggtgccc gagtgggtta cattgaactg gatctcaaca
     1741 gcggcaaaat cttagagagt tttcgccccg aagaacgctt cccaatgatg agcactttca
     1801 aagttctgct atgtggcgcg gtgttatccc gtattgatgc tgggcaagag caactcggtc
     1861 gccgcataca ctattctcag aatgacttgg ttgagtattc accagtcaca gaaaagcatc
     1921 ttacggatgg catgacagta agagaattat gtagtgctgc cataaccatg agtgataaca
     1981 ctgcggccaa cttacttctg acaacgattg gaggaccgaa ggagctaacc gcttttttgc
     2041 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2101 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaact ctccgcaaac
     2161 tattaactgg cgaactactt actcttgctt cacgccagca actcattgac tggatggagg
     2221 cggacaaagt tgcaggacca cttctgcgct cggcacttcc ggctggctgg tttattgctg
     2281 ataaatctgg agccggtgag cgtggctctc gcggtatcat tgcagcactg gggccagatg
     2341 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2401 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacg
     2461 aagggaaata aatagtagcc cgcctgatgt gcgggctttt ttttggtggt ggttaccagt
     2521 gtcgcttagc taccgtaggt cctcttaggc ggtccgatta gcgacatagt catcaat
//
