LOCUS       sequence-243256-        4157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35S::IPT::pinII terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM107
FEATURES             Location/Qualifiers
     source          1..4157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      792..1380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1281..1300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1534..1551
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2086..2431
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2374..2394
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        3777..3881
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atacactatt ctcagaatga cttggttgag tattcaccag tcacagaaaa gcatcttacg
       61 gatggcatga cagtaagaga attatgtagt gctgccataa ccatgagtga taacactgcg
      121 gccaacttac ttctgacaac gattggagga ccgaaggagc taaccgcttt tttgcacaac
      181 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      241 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caactctccg caaactatta
      301 actggcgaac tacttactct tgcttcacgc cagcaactca ttgactggat ggaggcggac
      361 aaagttgcag gaccacttct gcgctcggca cttccggctg gctggtttat tgctgataaa
      421 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      481 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
      541 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agacgaaggg
      601 aaataaatag tagcccgcct gatgtgcggg cttttttttg gtggtggtta ccagtgtcgc
      661 ttagctaccg taggtcctct taggcggtcc gattagcgac atagtcatca atgccttgac
      721 ggcaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
      781 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      841 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc tactaactct ttttccgaag
      901 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      961 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1021 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1081 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgctcaca gcccagcttg
     1141 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1201 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1261 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1321 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1381 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1441 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1501 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1561 gagcgcccac ctgcaacgcc ggcgtaatat ggcgcgccag atttgccttt tcaatttcag
     1621 aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtatca tcaagacgat
     1681 ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt
     1741 caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag
     1801 aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga
     1861 gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca
     1921 aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt
     1981 acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta
     2041 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
     2101 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
     2161 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
     2221 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     2281 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
     2341 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
     2401 ataaggaagt tcatttcatt tggagagaac acgggggact cctgcaggat ggatctgcgt
     2461 ctaattttcg gtccaacttg cacaggaaag acgtcgaccg cgatacgtct tgcccagcag
     2521 actggccttc cagtcctttc gctcgatcgg gtccaatgct gtcctcaact gtcaaccgga
     2581 agcggacgac caacagtgga agaactgaaa ggaacgaccc gtctatacct tgaagatcgg
     2641 cctctggtga agggtatcat cgcagccaag caagctcacg aaaggctgat cggggaagtg
     2701 tacaattatg aggcccacgg cgggcttatt cttgagggag gatctatctc gttgctcagg
     2761 tgcatggcgc aaagcagtta ttggagtacc gattttcgtt ggcatattat tcgccacaag
     2821 ttagcagacg aggagacatt catgaacgcg gccaaggcca gagttaggca gatgttgcgc
     2881 cctgctgtag gcccatctat tattcaagag ttggttcatc tttggaatga gcctcggctg
     2941 aggcccatac tgaaagagat cgacggatat cgatatgcca tgttatttgc tagccagaac
     3001 cagatcacac ccgatatgct attgcagctt gacccagata tggagggtga gttgattcat
     3061 ggaatcgctc aggagtatct catccatgcg cgccggcagg agcaggaatt ccctccagtg
     3121 agcgtggtcg ctttcgaagg attcgaaggt ccaccgttcg gaatgtgcta gctcgagccc
     3181 tagacttgtc catcttctgg attggccaag ttaattaatg tatgaaataa aaggatgcac
     3241 acatagtgac atgctaatca ctataatgtg ggcatcaaag ttgtgtgtta tgtgtaatta
     3301 ctaattatct gaataagaga aagagatcat ccatatttct tatcctaaat gaatgtcacg
     3361 tgtctttata attctttgat gaaccagatg cattttatta accaattcca tatacatata
     3421 aatattaatc atatataatt aatatcaatt gggttagcaa aacaaatcta gtctaggtgt
     3481 gttttgctaa ttattggggg atagtgcaaa aagaaatcta cgttctcaat aattcagata
     3541 gaaaacttaa taaagtgaga taatttacat agattgcttt tatcctttga tatatgtgaa
     3601 accatgcatg atataaggaa aatagataga gaaataattt tttacatcgt tgaatatgta
     3661 aacaatttaa ttcaagaagc taggaatata aatattgagg agtttatgat tagagctctc
     3721 ccactagcag gtgatgaaaa gccttagggt caggtggcac ttttcgggga aatgtgcgat
     3781 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgcta atgagacaat
     3841 aaccctgata aatgcttcaa ttatattgaa aaaggaagag tatgagtatc caacatttcc
     3901 gtgtcgccct tattcccttt tttgcggcct tctgccttcc tgtttttgct cacccagaaa
     3961 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc ccgagtgggt tacattgaac
     4021 tggatctcaa cagcggcaaa atcttagaga gttttcgccc cgaagaacgc ttcccaatga
     4081 tgagcacttt caaagttctg ctatgtggcg cggtgttatc ccgtattgat gctgggcaag
     4141 agcaactcgg tcgccgc
//
