LOCUS       sequence-243257-        3828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CmYLCV::STM::35S terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pRN110
FEATURES             Location/Qualifiers
     source          1..3828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1873..2047
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        2116..2220
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(3288..3828,1..48)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3777..3796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      181 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccacct
      241 gcaacgtccc gctggcagac atactgtccc acaaatgaag atggaatctg taaaagaaaa
      301 cgcgtgaaat aatgcgtctg acaaaggtta ggtcggctgc ctttaatcaa taccaaagtg
      361 gtccctacca cgatggaaaa actgtgcagt cggtttggct ttttctgacg aacaaataag
      421 attcgtggcc gacaggtggg ggtccaccat gtgaaggcat cttcagactc caataatgga
      481 gcaatgacgt aagggcttac gaaataagta agggtagttt gggaaatgtc cactcacccg
      541 tcagtctata aatacttagc ccctccctca ttgttaaggg agcaaaatct cagagagata
      601 gtcctagaga gagaaagaga gcaagtagcc tagaagtagt caaggcggcg aagtattcag
      661 gcacgtggcc aggaagaaga aaagccaaga cgacgaaaac aggtaagagc taagcttatg
      721 gagagtggtt ccaacagcac ttcttgtcca atggcttttg ccggggataa tagtgatggt
      781 ccgatgtgtc ctatgatgat gatgatgccg cccatcatga catcacatca acatcatggt
      841 catgatcatc aacatcaaca acaagaacat gatggttatg catatcagtc acaccaccaa
      901 caaagtagtt ccctttttct tcaatcacta gctcctcccc aaggaactaa gaacaaagtt
      961 gcttcttctt cttctccttc ctcttgtgct cctgcctatt ctctaatgga gatccatcat
     1021 aacgaaatcg ttgcaggagg aatcaaccct tgctcctctt cctcttcttc agcctctgtc
     1081 aaggccaaga tcatggctca tcctcactac caccgcctct tggccgctta tgtcaattgt
     1141 cagaaggttg gagcaccacc ggaggttgtg gcgaggctag aggaggcatg ctcgtctgcc
     1201 gcagccgctg ccgcatctat gggaccaaca ggatgtctag gtgaagatcc agggcttgat
     1261 caattcatgg aagcttactg tgaaatgctc gttaagtatg agcaagagct ctccaaacct
     1321 ttcaaggaag ctatggtctt ccttcaacgt gtcgagtgtc aattcaaatc cctctctcta
     1381 tcctcacctt cctctttctc cggttatgga gagacagcaa ttgataggaa caataatggg
     1441 tcatccgagg aagaagtcga tatgaacaat gaatttgtag atccacaagc tgaggataga
     1501 gagcttaaag gacagctctt gcgcaagtac agtggttact tagggagcct caagcaagag
     1561 ttcatgaaga agaggaagaa aggaaagctc cctaaagaag ctcgtcaaca actgcttgat
     1621 tggtggagcc gtcactacaa atggccttac ccttcggagc aacaaaagct cgcccttgcg
     1681 gaatcaacgg ggctggacca gaaacagata aacaattggt tcataaacca gaggaaacgg
     1741 cattggaagc cgtcggagga catgcagttt gtagtaatgg acgcaacaca tcctcaccat
     1801 tacttcatgg ataatgtctt gggcaatcct ttcccaatgg atcacatctc ctccaccatg
     1861 ctttgactcg agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt
     1921 tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
     1981 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat
     2041 ccagatcccc cgaattaagt gactagcagg tgatgaaaag ccttagggtc aggtggcact
     2101 tttcggggaa atgtgcgatg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2161 tatccgctaa tgagacaata accctgataa atgcttcaat tatattgaaa aaggaagagt
     2221 atgagtatcc aacatttccg tgtcgccctt attccctttt ttgcggcctt ctgccttcct
     2281 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcc
     2341 cgagtgggtt acattgaact ggatctcaac agcggcaaaa tcttagagag ttttcgcccc
     2401 gaagaacgct tcccaatgat gagcactttc aaagttctgc tatgtggcgc ggtgttatcc
     2461 cgtattgatg ctgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2521 gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2581 tgtagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatt
     2641 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2701 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2761 cctgtagcaa tggcaacaac tctccgcaaa ctattaactg gcgaactact tactcttgct
     2821 tcacgccagc aactcattga ctggatggag gcggacaaag ttgcaggacc acttctgcgc
     2881 tcggcacttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2941 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3001 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3061 tcactgatta agcattggta actgtcagac gaagggaaat aaatagtagc ccgcctgatg
     3121 tgcgggcttt tttttggtgg tggttaccag tgtcgcttag ctaccgtagg tcctcttagg
     3181 cggtccgatt agcgacatag tcatcaatgc cttgacggca aaatccctta acgtgagttt
     3241 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3301 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3361 ttgccggatc aagagctact aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3421 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3481 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3541 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3601 ggctgaacgg ggggttcgtg ctcacagccc agcttggagc gaacgaccta caccgaactg
     3661 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3721 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3781 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgactt
//
