LOCUS       sequence-243258-        4149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35S::IPT::pinII terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM112
FEATURES             Location/Qualifiers
     source          1..4149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      43..631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     532..551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     785..802
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1329..1674
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1617..1637
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        3020..3124
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
       61 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      121 ggatcaagag ctactaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      181 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      241 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      301 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      361 aacggggggt tcgtgctcac agcccagctt ggagcgaacg acctacaccg aactgagata
      421 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      481 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      541 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      601 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      661 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      721 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
      781 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca cctgcaacgt cccggcgcgc
      841 cagatttgcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      901 gcagcaggta tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      961 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
     1021 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1081 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1141 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1201 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1261 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1321 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1381 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1441 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1501 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1561 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1621 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1681 actcctgcag gatggatctg cgtctaattt tcggtccaac ttgcacagga aagacgtcga
     1741 ccgcgatacg tcttgcccag cagactggcc ttccagtcct ttcgctcgat cgggtccaat
     1801 gctgtcctca actgtcaacc ggaagcggac gaccaacagt ggaagaactg aaaggaacga
     1861 cccgtctata ccttgaagat cggcctctgg tgaagggtat catcgcagcc aagcaagctc
     1921 acgaaaggct gatcggggaa gtgtacaatt atgaggccca cggcgggctt attcttgagg
     1981 gaggatctat ctcgttgctc aggtgcatgg cgcaaagcag ttattggagt accgattttc
     2041 gttggcatat tattcgccac aagttagcag acgaggagac attcatgaac gcggccaagg
     2101 ccagagttag gcagatgttg cgccctgctg taggcccatc tattattcaa gagttggttc
     2161 atctttggaa tgagcctcgg ctgaggccca tactgaaaga gatcgacgga tatcgatatg
     2221 ccatgttatt tgctagccag aaccagatca cacccgatat gctattgcag cttgacccag
     2281 atatggaggg tgagttgatt catggaatcg ctcaggagta tctcatccat gcgcgccggc
     2341 aggagcagga attccctcca gtgagcgtgg tcgctttcga aggattcgaa ggtccaccgt
     2401 tcggaatgtg ctagctcgag ccctagactt gtccatcttc tggattggcc aagttaatta
     2461 atgtatgaaa taaaaggatg cacacatagt gacatgctaa tcactataat gtgggcatca
     2521 aagttgtgtg ttatgtgtaa ttactaatta tctgaataag agaaagagat catccatatt
     2581 tcttatccta aatgaatgtc acgtgtcttt ataattcttt gatgaaccag atgcatttta
     2641 ttaaccaatt ccatatacat ataaatatta atcatatata attaatatca attgggttag
     2701 caaaacaaat ctagtctagg tgtgttttgc taattattgg gggatagtgc aaaaagaaat
     2761 ctacgttctc aataattcag atagaaaact taataaagtg agataattta catagattgc
     2821 ttttatcctt tgatatatgt gaaaccatgc atgatataag gaaaatagat agagaaataa
     2881 ttttttacat cgttgaatat gtaaacaatt taattcaaga agctaggaat ataaatattg
     2941 aggagtttat gattagagct cagtgactag caggtgatga aaagccttag ggtcaggtgg
     3001 cacttttcgg ggaaatgtgc gatgaacccc tatttgttta tttttctaaa tacattcaaa
     3061 tatgtatccg ctaatgagac aataaccctg ataaatgctt caattatatt gaaaaaggaa
     3121 gagtatgagt atccaacatt tccgtgtcgc ccttattccc ttttttgcgg ccttctgcct
     3181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3241 tgcccgagtg ggttacattg aactggatct caacagcggc aaaatcttag agagttttcg
     3301 ccccgaagaa cgcttcccaa tgatgagcac tttcaaagtt ctgctatgtg gcgcggtgtt
     3361 atcccgtatt gatgctgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3421 cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3481 attatgtagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3541 gattggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3661 gatgcctgta gcaatggcaa caactctccg caaactatta actggcgaac tacttactct
     3721 tgcttcacgc cagcaactca ttgactggat ggaggcggac aaagttgcag gaccacttct
     3781 gcgctcggca cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3961 tgcctcactg attaagcatt ggtaactgtc agacgaaggg aaataaatag tagcccgcct
     4021 gatgtgcggg cttttttttg gtggtggtta ccagtgtcgc ttagctaccg taggtcctct
     4081 taggcggtcc gattagcgac atagtcatca atgccttgac ggcaaaatcc cttaacgtga
     4141 gttttcgtt
//
