LOCUS       sequence-243259-        2041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty Backbone for golden gate cloning compatible with Cermak., et 
            al Toolkit. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMOD_D0000a
FEATURES             Location/Qualifiers
     source          1..2041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        329..433
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(1501..2041,1..48)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1990..2009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      181 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccacct
      241 gcaacgtccc ggcgcgccga tatcgagctc agtgactagc aggtgatgaa aagccttagg
      301 gtcaggtggc acttttcggg gaaatgtgcg atgaacccct atttgtttat ttttctaaat
      361 acattcaaat atgtatccgc taatgagaca ataaccctga taaatgcttc aattatattg
      421 aaaaaggaag agtatgagta tccaacattt ccgtgtcgcc cttattccct tttttgcggc
      481 cttctgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      541 tcagttgggt gcccgagtgg gttacattga actggatctc aacagcggca aaatcttaga
      601 gagttttcgc cccgaagaac gcttcccaat gatgagcact ttcaaagttc tgctatgtgg
      661 cgcggtgtta tcccgtattg atgctgggca agagcaactc ggtcgccgca tacactattc
      721 tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac
      781 agtaagagaa ttatgtagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      841 tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      901 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      961 tgacaccacg atgcctgtag caatggcaac aactctccgc aaactattaa ctggcgaact
     1021 acttactctt gcttcacgcc agcaactcat tgactggatg gaggcggaca aagttgcagg
     1081 accacttctg cgctcggcac ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1141 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1201 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1261 tgagataggt gcctcactga ttaagcattg gtaactgtca gacgaaggga aataaatagt
     1321 agcccgcctg atgtgcgggc ttttttttgg tggtggttac cagtgtcgct tagctaccgt
     1381 aggtcctctt aggcggtccg attagcgaca tagtcatcaa tgccttgacg gcaaaatccc
     1441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1561 agcggtggtt tgtttgccgg atcaagagct actaactctt tttccgaagg taactggctt
     1621 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1801 ggcgcagcgg tcgggctgaa cggggggttc gtgctcacag cccagcttgg agcgaacgac
     1861 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2041 t
//
