LOCUS       sequence-24330-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  TITLE     T4 Lysozyme Plasmids - Tray 4
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme R119-delta WT*   27 OR 16
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(708..730)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    719..735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tctgaaaaag cccgctcatt aggcgggctc agatctgctc atgtttgaca gcttatcatc
       61 gataagcttc ggcaataaat tcttgatatg ttttcatatg tttcctttaa atattttaat
      121 tagtaattgt ctattcaagt aattgtgaat atactatcac aattccaaga gaaagtaaac
      181 agctttatag atttttatac gcgtcccaag tgccagttct aaacgttgta atgactcgtt
      241 ttgcgcgatt aggtgtttga ttataccatc tacttttagc taagttaact gctgcttcat
      301 cccagcgttt ttgttgaagc attaaagagt tagtaaatcc tgccacaccg gtttctccca
      361 tttggaaaac catattaatc aatgcagcgc gacgaaccgc atcaagagaa tcataaaccg
      421 gttttaattt agcatttctc agaattccgc gaacagcagc atcaacatcc tgattaaaga
      481 gtttttcagc ctcatctttt gtaattacac cattagtatt acgcccaata gctttatcta
      541 attcagattt agcagcatta agtgatggac tttttgtaag caaatgaccg atgccaatag
      601 tgtaatagcc ttctgtgtct ttatagattt taagtctaag accttcatct atacgtaaca
      661 tttcaaatat attcataata cctcctaagc atcgatggat cctgnttcct gtgtgaaatt
      721 gttatccgct cacaatccac acattatacg agccgatgat aattgtcaca gggggngggg
      781 agtagctgat cnggttcngt gtga
//
