LOCUS       sequence-243302-        9647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Temperature sensitive plasmid for either lambda Red recombinase 
            genes or flippase recombinase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Jensen SI, Lennen RM, Herrgard MJ, Nielsen AT
  TITLE     Seven gene deletions in seven days: Fast generation of Escherichia 
            coli strains tolerant to acetate and osmotic stress.
  JOURNAL   Sci Rep. 2015 Dec 8;5:17874. doi: 10.1038/srep17874.
  PUBMED    26643270
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-12-8"; volume: "5:17874. doi"; pages: " 
            10.1038/srep17874"
COMMENT     Sequence Label: pSIJ8
FEATURES             Location/Qualifiers
     source          1..9647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     512..531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(750..854)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(891..1769)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1796..2080
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2003..2022
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             2107..2115
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2124..2540
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             2546..3331
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             3328..4008
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      4009..4253
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     CDS             complement(4376..5647)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     promoter        complement(5671..5789)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             5935..6771
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     CDS             6845..7693
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEYESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(7694..8644)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      8692..8914
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(join(9536..9647,1..680,681..749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
       61 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
      121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
      181 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
      301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
      361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
      421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
      481 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
      541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
      601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
      661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
      721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
      781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
      841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg catcgattta ttatgacaac
      901 ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc
      961 ccggtgcatt ttttaaatac ccgcgagaag tagagttgat cgtcaaaacc aacattgcga
     1021 ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt
     1081 tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga
     1141 cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc
     1201 aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc
     1261 gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc
     1321 agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg
     1381 aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc
     1441 cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat
     1501 tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata
     1561 tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg
     1621 gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa
     1681 ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc
     1741 agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa
     1801 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg
     1861 ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc
     1921 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat
     1981 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
     2041 acctgacgct ttttatcgca actctctact gtttctccat acccgttttt ttgggaattc
     2101 gagctctaag gaggttataa aaaatggata ttaatactga aactgagatc aagcaaaagc
     2161 attcactaac cccctttcct gttttcctaa tcagcccggc atttcgcggg cgatattttc
     2221 acagctattt caggagttca gccatgaacg cttattacat tcaggatcgt cttgaggctc
     2281 agagctgggc gcgtcactac cagcagctcg cccgtgaaga gaaagaggca gaactggcag
     2341 acgacatgga aaaaggcctg ccccagcacc tgtttgaatc gctatgcatc gatcatttgc
     2401 aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt tgatgacgat gttgagtttc
     2461 aggagcgcat ggcagaacac atccggtaca tggttgaaac cattgctcac caccaggttg
     2521 atattgattc agaggtataa aacgaatgag tactgcactc gcaacgctgg ctgggaagct
     2581 ggctgaacgt gtcggcatgg attctgtcga cccacaggaa ctgatcacca ctcttcgcca
     2641 gacggcattt aaaggtgatg ccagcgatgc gcagttcatc gcattactga tcgttgccaa
     2701 ccagtacggc cttaatccgt ggacgaaaga aatttacgcc tttcctgata agcagaatgg
     2761 catcgttccg gtggtgggcg ttgatggctg gtcccgcatc atcaatgaaa accagcagtt
     2821 tgatggcatg gactttgagc aggacaatga atcctgtaca tgccggattt accgcaagga
     2881 ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa tgccgccgcg aaccattcaa
     2941 aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg catcccaaac ggatgttacg
     3001 tcataaagcc atgattcagt gtgcccgtct ggccttcgga tttgctggta tctatgacaa
     3061 ggatgaagcc gagcgcattg tcgaaaatac tgcatacact gcagaacgtc agccggaacg
     3121 cgacatcact ccggttaacg atgaaaccat gcaggagatt aacactctgc tgatcgccct
     3181 ggataaaaca tgggatgacg acttattgcc gctctgttcc cagatatttc gccgcgacat
     3241 tcgtgcatcg tcagaactga cacaggccga agcagtaaaa gctcttggat tcctgaaaca
     3301 gaaagccgca gagcagaagg tggcagcatg acaccggaca ttatcctgca gcgtaccggg
     3361 atcgatgtga gagctgtcga acagggggat gatgcgtggc acaaattacg gctcggcgtc
     3421 atcaccgctt cagaagttca caacgtgata gcaaaacccc gctccggaaa gaagtggcct
     3481 gacatgaaaa tgtcctactt ccacaccctg cttgctgagg tttgcaccgg tgtggctccg
     3541 gaagttaacg ctaaagcact ggcctgggga aaacagtacg agaacgacgc cagaaccctg
     3601 tttgaattca cttccggcgt gaatgttact gaatccccga tcatctatcg cgacgaaagt
     3661 atgcgtaccg cctgctctcc cgatggttta tgcagtgacg gcaacggcct tgaactgaaa
     3721 tgcccgttta cctcccggga tttcatgaag ttccggctcg gtggtttcga ggccataaag
     3781 tcagcttaca tggcccaggt gcagtacagc atgtgggtga cgcgaaaaaa tgcctggtac
     3841 tttgccaact atgacccgcg tatgaagcgt gaaggcctgc attatgtcgt gattgagcgg
     3901 gatgaaaagt acatggcgag ttttgacgag atcgtgccgg agttcatcga aaaaatggac
     3961 gaggcactgg ctgaaattgg ttttgtattt ggggagcaat ggcgatgacg catcctcacg
     4021 ataatatccg ggtaggcgca atcactttcg tctactccgt tacaaagcga ggctgggtat
     4081 ttcccggcct ttctgttatc cgaaatccac tgaaagcaca gcggctggct gaggagataa
     4141 ataataaacg aggggctgta tgcacaaagc atcttctgtt gagttaagaa cgagtatcga
     4201 gatggcacat agccttgctc aaattggaat caggtttgtg ccaataccag tagaaacaga
     4261 cgaagaatcc atgggtatgg acagttttcc ctttgatatg taacggtgaa cagttgttct
     4321 acttttgttt gttagtcttg atgcttcact gatagataca agagccataa gaaccttata
     4381 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc
     4441 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact
     4501 cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatatg
     4561 catagtaccg agaaactagt gcgaagtagt gatcaggtat tgctgttatc tgatgagtat
     4621 acgttgtcct ggccacggca gaagcacgct tatcgctcca atttcccaca acattagtca
     4681 actccgttag gcccttcatt gaaagaaatg aggtcatcaa atgtcttcca atgtgagatt
     4741 ttgggccatt ttttatagca aagattgaat aaggcgcatt tttcttcaaa gctttattgt
     4801 acgatctgac taagttatct tttaataatt ggtattcctg tttattgctt gaagaattgc
     4861 cggtcctatt tactcgtttt aggactggtt cagaattcct caaaaattca tccaaatata
     4921 caagtggatc gatcctaccc cttgcgctaa agaagtatat gtgcctacta acgcttgtct
     4981 ttgtctctgt cactaaacac tggattatta ctcccagata cttattttgg actaatttaa
     5041 atgatttcgg atcaacgttc ttaatatcgc tgaatcttcc acaattgatg aaagtagcta
     5101 ggaagaggaa ttggtataaa gtttttgttt ttgtaaatct cgaagtatac tcaaacgaat
     5161 ttagtatttt ctcagtgatc tcccagatgc tttcaccctc acttagaagt gctttaagca
     5221 tttttttact gtggctattt cccttatctg cttcttccga tgattcgaac tgtaattgca
     5281 aactacttac aatatcagtg atatcagatt gatgtttttg tccatagtaa ggaataattg
     5341 taaattccca agcaggaatc aatttcttta atgaggcttc cagaattgtt gctttttgcg
     5401 tcttgtattt aaactggagt gatttattga caatatcgaa actcagcgaa ttgcttatga
     5461 tagtattata gctcatgaat gtggctctct tgattgctgt tccgttatgt gtaatcatcc
     5521 aacataaata ggttagttca gcagcacata atgctatttt ctcacctgaa ggtctttcaa
     5581 acctttccac aaactgacga acaagcacct taggtggtgt tttacataat ataccaaatt
     5641 gtggcatcta gtatttctcc tcaatttcat tacgaccagt ctaaaaagcg cctgaattcg
     5701 cgaccttctc gttactgaca ggaaaatggg ccattggcaa ccagggaaag atgaacgtga
     5761 tgatgttcac aatttgctga attgtggtga tgtgatgctc accgcatttc ctgaaaattc
     5821 acgctgtatc ttgaaaaatc gacgtttttt acgtggtttt ccgtcgaaaa tttaaggtaa
     5881 gaacctgacc tcgtgattac tatttcgccg tgttgacgac atcaggaggc cagtatgacc
     5941 gtattacata gtgtggattt ttttccgtct ggtaacgcgt ccgtggcgat agaaccccgg
     6001 ctcccgcagg cggattttcc tgaacatcat catgattttc atgaaattgt gattgtcgaa
     6061 catggcacgg gtattcatgt gtttaatggg cagccctata ccatcaccgg tggcacggtc
     6121 tgtttcgtac gcgatcatga tcggcatctg tatgaacata ccgataatct gtgtctgacc
     6181 aatgtgctgt atcgctcgcc ggatcgattt cagtttctcg ccgggctgaa tcagttgctg
     6241 ccacaagagc tggatgggca gtatccgtct cactggcgcg ttaaccacag cgtattgcag
     6301 caggtgcgac agctggttgc acagatggaa cagcaggaag gggaaaatga tttaccctcg
     6361 accgccagtc gcgagatctt gtttatgcaa ttactgctct tgctgcgtaa aagcagtttg
     6421 caggagaacc tggaaaacag cgcatcacgt ctcaacttgc ttctggcctg gctggaggac
     6481 cattttgccg atgaggtgaa ttgggatgcc gtggcggatc aattttctct ttcactgcgt
     6541 acgctacatc ggcagcttaa gcagcaaacg ggactgacgc ctcagcgata cctgaaccgc
     6601 ctgcgactga tgaaagcccg acatctgcta cgccacagcg aggccagcgt tactgacatc
     6661 gcctatcgct gtggattcag cgacagtaac cacttttcga cgctttttcg ccgagagttt
     6721 aactggtcac cgcgtgatat tcgccaggga cgggatggct ttctgcaata acgcgaatct
     6781 tctcaacgta tttgtacgcc atattgcgaa taatcaactt cgttctctgg ccgaggtagc
     6841 cacggtggcg catcagttaa aacttctcaa agatgatttt tttgccagcg accagcaggc
     6901 agtcgctgtg gctgaccgtt atccgcaaga tgtctttgct gaacatacac atgatttttg
     6961 tgagctggtg attgtctggc gcggtaatgg cctgcatgta ctcaacgatc gcccttatcg
     7021 cattacccgt ggcgatctct tttacattca tgctgacgat aaacactcct acgcttccgt
     7081 taacgatctg gttttgcaga atattattta ttgcccggag cgtctgaagc tgaatcttga
     7141 ctggcagggg gcgattccgg gatttaacgc cagcgcaggg caaccacact ggcgcttagg
     7201 tagcatgggg atggcgcagg cgcggcaggt tatcggtcag cttgagtatg aaagtagtca
     7261 gcatgtgccg tttgctaacg aaatggctga gttgctgttc gggcagttgg tgatgttgct
     7321 gaatcgccat cgttacacca gtgattcgtt gccgccaaca tccagcgaaa cgttgctgga
     7381 taagctgatt acccggctgg cggctagcct gaaaagtccc tttgcgctgg ataaattttg
     7441 tgatgaggca tcgtgcagtg agcgcgtttt gcgtcagcaa tttcgccagc agactggaat
     7501 gaccatcaat caatatctgc gacaggtcag agtgtgtcat gcgcaatatc ttctccagca
     7561 tagccgcctg ttaatcagtg atatttcgac cgaatgtggc tttgaagata gtaactattt
     7621 ttcggtggtg tttacccggg aaaccgggat gacgcccagc cagtggcgtc atctcaattc
     7681 gcagaaagat taatcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt
     7741 gtttttgcgt gagccatgag aacgaaccat tgagatcata cttactttgc atgtcactca
     7801 aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt
     7861 ttcttagtcc gttatgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat
     7921 tcatttttat ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa
     7981 cttggaaaat caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc
     8041 tgtaagtgtt taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact
     8101 catggtagtt attttcaagc attaacatga acttaaattc atcaaggcta atctctatat
     8161 ttgccttgtg agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag
     8221 agtatttgtt ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact
     8281 ggaaaagata aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact
     8341 tggcatagtt tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca
     8401 cagttctcgt catcagctct ctggttgctt tagctaatac accataagca ttttccctac
     8461 tgatgttcat catctgaacg tattggttat aagtgaacga taccgtccgt tctttccttg
     8521 tagggttttc aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat
     8581 gctccgttaa gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag
     8641 acatacatct caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc
     8701 aatgataatt actagtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct
     8761 ttgctggaaa acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg
     8821 ttttttttgt ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga
     8881 taaaaagaat agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt
     8941 acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag
     9001 gcttaagtag caccctcgca agctcggttg cggccgcaat cgggcaaatc gctgaatatt
     9061 ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc
     9121 tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc ttttatggat
     9181 tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct cagggcgttt
     9241 tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt tcctgccctc
     9301 tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga ctggctaatg
     9361 cacccagtaa ggcagcggta tcatcaacgg ggtctgacgc tcagtggaac gaaaactcac
     9421 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9481 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9541 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9601 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttacca
//
