LOCUS       sequence-243304-       12479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gateway cloning destination vector to express LexAop2 driven 
            transgene in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Poe AR, Tang L, Wang B, Li Y, Sapar ML, Han C
  TITLE     Dendritic space-filling requires a neuronal type-specific 
            extracellular permissive signal in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Sep 19;114(38):E8062-E8071. doi: 
            10.1073/pnas.1707467114. Epub 2017 Sep 5.
  PUBMED    28874572
REFERENCE   2  (bases 1 to 12479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1707467114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-09-19- 19"; volume: "114"; issue: "38"; pages: 
            "E8062-E8071"
COMMENT     Sequence Label: pDEST-APLO
FEATURES             Location/Qualifiers
     source          1..12479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    415..800
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_feature    join(1198..1399,1400..1430)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    1974..2043
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2252..2271)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2371..2393
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2431..2449)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2517..2621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2622..2690,2691..3482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2840..2859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3653..4241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4142..4161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(4476..4506,4507..5061)
                     /label=P element 5' end
     CDS             complement(join(5840..6454,6525..6656,6860..7175,7237..7891,
                     7966..8239,8641..8712))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9164..9182
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    9192..9577
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    10246..10370
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(10411..10716)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(10513..10532)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(11058..11717)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     11632..11651
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(11771..11777,11778..11795,11796..11801))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(11826..11950)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(12001..12020)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          12003..12135
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(12231..12252)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 cttactgtat ggatgtacag tacttactgt atggatgtac agtacttact gtatggatgt
       61 acagtactta ctgtatggat gtacagtaac ctgcaggcat gcgctagcgc tagagtctcc
      121 gcttactgta tggatgtaca gtacttactg tatggatgta cagtacttac tgtatggatg
      181 tacagtaacc tgcaggcatg ccctagggct agagtctccg cttactgtat ggatgtacag
      241 tacttactgt atggatgtac agtacttact gtatggatgt acagtactta ctgtatggat
      301 gtacagtact tactgtatgg atgtacagta acctgcaggc atgcaagctc ggcgcgccgg
      361 cgaattcgcc cgggctctag accagatctg ctgcagcatg ctgttgccga gcacaattga
      421 tcggctaaat ggtatggcaa gaaaaggtat gcaatataat aatcttttat tgggtatgca
      481 acgaaaattt gtttcgtcaa cgtatgcaat attttttatt aaaagagggt atgcaatgta
      541 ttttattaaa aacgggtatg caatataata atcttttatt gggtatgcaa cgaaaatttg
      601 tttcgtcaaa gtatgcaata ttttttatta aaagagggta tgcaatgtat tttattaaaa
      661 acgggtatgc aataaaaaat tatttggttt ctctaaaaag tatgcagcac ttattttttg
      721 ataaggtatg caacaaaatt ttactttgcc gaaaatatgc aatgtttttg cgaataaatt
      781 caacgcacac ttattacgtg atgcagccaa gcttggcgaa cagttcggct ggcgcgagcc
      841 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
      901 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
      961 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     1021 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     1081 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     1141 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacctag gaagctttgc
     1201 gtactcgcaa attattaaaa ataaaacttt aaaaataatt tcgtctaatt aatattatga
     1261 gttaattcaa accccacgga catgctaagg gttaatcaac aatcatatcg ctgtctcact
     1321 cagactcaat acgacactca gaatactatt cctttcactc gcacttattg caagcatacg
     1381 ttaagtggat gtctcttgcc gacgggacca ccttatgtta tttcatcatg gtctggccat
     1441 tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa
     1501 ggagtagccg acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat
     1561 cgtgtttact gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa
     1621 acaagatatg acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt
     1681 tggatttgtc ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt
     1741 tatcgctaca taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc
     1801 ctcttcataa ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac
     1861 ccgctctccg caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc
     1921 cgcgcgattc ggtgcggtat ttcacaccgc atatcgacga tgtaggtcac ggtctcgaag
     1981 ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc
     2041 atctggtcca tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg
     2101 cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga
     2161 cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc
     2221 acggcgggca tgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     2281 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     2341 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     2401 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     2461 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     2521 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2581 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2641 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2701 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2761 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2821 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2881 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2941 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3001 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3061 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3121 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3181 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3241 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3301 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3361 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3421 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3481 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3541 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3601 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3661 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3721 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3781 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3841 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3901 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3961 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4021 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4081 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4141 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4201 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccttc ttcttgaact
     4261 cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca
     4321 acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat
     4381 cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg
     4441 ccgccgatct gccgctggac tacgtgggtc tggcccatga tgaaataaca taaggtggtc
     4501 ccgtcgatag ccgaagctta ccgaagtata cacttaaatt cagtgcacgt ttgcttgttg
     4561 agaggaaagg ttgtgtgcgg acgaattttt ttttgaaaac attaaccctt acgtggaata
     4621 aaaaaaaatg aaatattgca aattttgctg caaagctgtg actggagtaa aattaattca
     4681 cgtgccgaag tgtgctatta agagaaaatt gtgggagcag agccttgggt gcagccttgg
     4741 tgaaaactcc caaatttgtg atacccactt taatgattcg cagtggaagg ctgcacctgc
     4801 aaaaggtcag acatttaaaa ggaggcgact caacgcagat gccgtaccta gtaaagtgat
     4861 agagcctgaa ccagaaaaga taaaagaagg ctataccagt gggagtacac aaacagagta
     4921 agtttgaata gtaaaaaaaa tcatttatgt aaacaataac gtgactgtgc gttaggtcct
     4981 gttcattgtt taatgaaaat aagagcttga gggaaaaaat tcgtactttg gagtacgaaa
     5041 tgcgtcgttt agagcagcag ccgaattaat tctagttcca gtgaaatcca agcattttct
     5101 aaattaaatg tattcttatt attatagttg ttatttttga tatatataaa caacactatt
     5161 atgcccacca tttttttgag atgcatctac acaaggaaca aacactggat gtcactttca
     5221 gttcaaattg taacgctaat cactccgaac aggtcacaaa aaattacctt aaaaagtcat
     5281 aatattaaat tagaataaat atagctgtga gggaaatata tacaaatata ttggagcaaa
     5341 taaattgtac atacaaatat ttattactaa tttctattga gacgaaatga accactcgga
     5401 accatttgag cgaaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca
     5461 aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg
     5521 gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta tataaaaaat
     5581 atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg caggtgtgta
     5641 agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga aggggaaata
     5701 cttgtattct ataggtcata tcttgttttt attggcacaa atataattac attagctttt
     5761 tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa aacaagcagt
     5821 tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga gccagatatg
     5881 cgagcacccg gaagctcacg atgagaatgg ccagacccac gtagtccagc ggcagatcgg
     5941 cggcggagaa gttaagcgtc tccaggatga ccttgcccga actggggcac gtggtgttcg
     6001 acgatgtgca gctaatttcg cccggctcca cgtccgccca ttggttaatc agcagaccct
     6061 cgttggcgta acggaaccat gagaggtacg acaaccattt gaggtatact ggcaccgagc
     6121 ccgagttcaa gaagaagccg ccaaagagca ggaatggtat gataaccggc ggacccacag
     6181 acagcgccat cgaggtcgag gagctggcgc aggatattag atatccgaag gacgttgaca
     6241 cattggccac cagagtgacc agcgccaggc agttgaagaa gtgcagcact ccggcccgca
     6301 gtccgatcat cggataggca atcgccgtga agaccagtgg cactgtgaga aaaagcggca
     6361 attcggcaat cgttttgccc agaaagtatg tgtcacagcg ataaagtcga cttcgggcct
     6421 ccctcataaa aactggcagc tctgaggtga acacctaaat cgaatcgatt cattagaaag
     6481 ttagtaaatt attgaaatgc aaatgtattc taaacatgac ttacatttat cgtggcaaag
     6541 acgttttgaa aggtcatgtt ggtcaggaag aggaagatgg ctccgttgat attcatcaca
     6601 cccacttgcg tgagttgttg gcccaaaaag atgaggccaa tcaagatggc aaccatctgc
     6661 aaattaaaat gttactcgca tctcattaat attcgcgagt taaatgaaat ttatttatct
     6721 tctgcaaaac tataaactat acatctcatt gaaaaaaact aagaagggtg tggaatcagg
     6781 caattctatc taaaatctag cgaatttgtt tccaagaatt gtaagcgtta tatcatttgt
     6841 ttccactgga accactcacc gttgtctgaa taagtcgcac ttttacgagg agtggttcct
     6901 tgagcaccga cagccaggat cgccacagga ccgcccggaa ctgcatgaac caggtggcct
     6961 tgtaggtgta cccattctcc ggctgctcca gtggcttctc cagatttttg gtggccaaca
     7021 actgctccat atcccgggct actttgctaa tggcaaaatt gtcgcatatc ttggcgatcc
     7081 gatcacggga ctcgatctcc cgtccgggca caacggccaa cacctgtacg taaaagtccg
     7141 ccggattgta gttggtagga cactgggcac ccacgctgga taggagttga gatgttatgt
     7201 aatactagat acccttaata aacacatcga actcactagg aaaagaagtc gacggcttcg
     7261 ctgggagtgc ccaagaaagc taccctgccc tcggccatca gaaggatctt gtcaaagagc
     7321 tcaaacagct cggaagacgg ctgatgaatg gtcaggatga cggtcttgcc cttctgcgac
     7381 agcttcttca gcacctggac gacgctgtgg gcggtaaagg agtccagtcc ggaggtgggc
     7441 tcatcgcaga tcagaagcgg cggatcggtt agagcctcgg aggcgaatgc cagacgcttc
     7501 ctttctccgc cggacagacc tttcaccctg ccgggcacac cgatgatcgt gtgctgacat
     7561 ttgctgagcg aaagctcctg gatcacctga tccacgcggg ccactcgctg ccgataggtc
     7621 agatgtcgtg gcatccgcac catggcttgg aaaatcaggt gttccctggc cgttagggag
     7681 ccgataaaga ggtcatcctg ctggacatag gcgcacctgg cctgcatctc cttggcgtcc
     7741 acaggttggc cattgagcag tcgcatcccg gatggcgata cttggatgcc ctgcggcgat
     7801 cgaaaggcaa gggcattcag cagggtcgtc tttccggcac cggaactgcc catcacggcc
     7861 aaaagttcgc ccggataggc cacgccgcaa actgagtttc aaattggtaa ttggaccctt
     7921 tattaagatt tcacacagat cagccgactg cgaatagaaa ctcaccgttc ttgagcaaat
     7981 gtttcctggg cgccggtatg tgtcgctcgt tgcagaatag tccgcgtgtc cggttgacca
     8041 gctgccgcca tccggagccc ggctgattga ccgccccaaa gatgtccata ttgtgccagg
     8101 cataggtgag gttctcggct agttggccgc tccctgaacc ggagtcctcc ggcggactgg
     8161 gtggccggag cgtgccgtag tttttggcct gcccgaagcc ctggttaatg cagctctgcg
     8221 aagccgctcc gctgtcaccc tgcaatgata ggggatctca aatatcaact acaagcgtta
     8281 tgctcatcta accccgaaca aaaagtaccc cgaagtatcc tacgaagtag gtttatactt
     8341 ttatttattt tttgtgcatc taggatcagc ttaaaatatc tggttgttat attttttgta
     8401 aaaaagaata tagtcgaaaa tgaatgcctt tagatgtctt gatcatgata tgatctcaaa
     8461 aattgtctta tatagcgaga acagctacca gaataatctg tttcgtgtca ctatttgttt
     8521 gtgcaattgc ggtttgggat ttttgtgggt cgcagttctc acgccgcaga caatttgatg
     8581 ttgcaatcgc agttcctata gatcaagtga acttaagatg tatgcacatg tactactcac
     8641 attgttcaga tgctcggcag atgggtgttt gctgcctccg cgaattaata gctcctgatc
     8701 ctcttggccc attgccggga tttttcacac tttcccctgc ttacccaccc aaaaccaatc
     8761 accaccccaa tcactcaaaa aacaaacaaa aataagaagc gagaggagtt ttggcacagc
     8821 actttgtgtt taattgatgg cgtaaaccgc ttggagcttc gtcacgaaac cgctgacaaa
     8881 atgcaactga aggcggacat tgacgctacg taacgctaca aacggtggcg aaagagatag
     8941 cggacgcagc ggcgaaagag acggcgatat ttctgtggac agagaaggag gcaaacagcg
     9001 ctgactttga gtggaatgtc attttgagtg agaggtaatc gaaagaacct ggtacatcaa
     9061 atacccttgg atcgaagtaa atttaaaact gatcagataa gttcaatgat atccagtgca
     9121 gtaaaaaaaa aaaatgtttt ttttatctac tttccgcaaa aatgggtttt attaacttac
     9181 atacatacta gaattgatcg gctaaatggt atggcaagaa aaggtatgca atataataat
     9241 cttttattgg gtatgcaacg aaaatttgtt tcgtcaacgt atgcaatatt ttttattaaa
     9301 agagggtatg caatgtattt tattaaaaac gggtatgcaa tataataatc ttttattggg
     9361 tatgcaacga aaatttgttt cgtcaaagta tgcaatattt tttattaaaa gagggtatgc
     9421 aatgtatttt attaaaaacg ggtatgcaat aaaaaattat ttggtttctc taaaaagtat
     9481 gcagcactta ttttttgata aggtatgcaa caaaatttta ctttgccgaa aatatgcaat
     9541 gtttttgcga ataaattcaa cgcacactta ttacgtgact agttacgcat tttgaaaaac
     9601 gtcaggtcag tctattcatt attatctttc ggatgaaaag ctacagttgt gatattttgc
     9661 tctaggcatt tacacgttta ttgggcttac aaatacattg ggttcgagct gctaaatgcc
     9721 ctgattaaaa ctgaacttta agcgatacta ctgcactttt ctcacaatta tgaaaaactt
     9781 gtacttctta cagctgaaat taattaccat ctatgtagtt gcaattcttt acattcatcg
     9841 acacaagcgc aaacatctcg tctagttttt tttttttttt ttttttttgg aaataacatg
     9901 attggcgttt ttattatagt ttttttttat taaatttatt cgtgtaatta ctttgctcta
     9961 aattaagttt tgtttttgtt tcatactcaa cctcgccaag gatgcgtgcg ggtaaatacg
    10021 ttttgtgtgt ttgtattttt tgtttgtttg tatgtgagtg ttggggagat gctagaatta
    10081 cataattatg tattacgttg tatataagaa atatatgctg gcgcacagaa atgattacaa
    10141 ctcgtcggtg ttttagcttg tctctgctga aatctgaatt aaacattagt gttgcatgtt
    10201 tcgaaggcgt ccgattgccg actggcttag ttaattaata ggatcaccac tttgtacaag
    10261 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
    10321 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc
    10381 agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc
    10441 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac
    10501 cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac
    10561 cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac
    10621 catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg
    10681 gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag
    10741 caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg
    10801 ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac
    10861 cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac
    10921 tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta
    10981 tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt
    11041 aagccggatc cacgcgttta cgccccgccc tgccactcat cgcagtactg ttgtaattca
    11101 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
    11161 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
    11221 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
    11281 gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
    11341 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
    11401 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
    11461 tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc
    11521 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
    11581 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
    11641 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
    11701 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg atcctaactc
    11761 aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt gcctaatgcg
    11821 gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
    11881 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gcttttttgt
    11941 acaaacttgt gatcgcgtac cggttttctc tcgagagctg taattgaact gggagtggac
    12001 acctgtggag agaaaggcaa agtggatgtc agtaagacca ataggtgcct atcagaaacg
    12061 caagagtctt ctctgtctcg acaagcccag tttctattgg tctccttaaa cctgtcttgt
    12121 aaccttgata cttacctgcc cagtgcctca cgaccaactt ctgcaaggct accttaagag
    12181 agtcactggt cgagtattca gagttctctc cttgtattca ataattactt cttggcagat
    12241 ttcagtagtt gcagttgatt tacttggttg ctggttactt ttaattgatt cactttaact
    12301 tgcactttac gattgtttag cttgctcagc tgcgcttgtt tatttgctta gctttcgctt
    12361 agcgacgtgt tcactttgct tgtttgaatt gaattgtcgc tccgtagacg aagcgcctct
    12421 atttatactc cggcgctcgc tagtgctaga gtctccgctt actgtatgga tgtacagta
//
