LOCUS       sequence-243307-       13143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gateway cloning destination vector to express enhancer driven Flip1 
            in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13143)
  AUTHORS   Poe AR, Tang L, Wang B, Li Y, Sapar ML, Han C
  TITLE     Dendritic space-filling requires a neuronal type-specific 
            extracellular permissive signal in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Sep 19;114(38):E8062-E8071. doi: 
            10.1073/pnas.1707467114. Epub 2017 Sep 5.
  PUBMED    28874572
REFERENCE   2  (bases 1 to 13143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1707467114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-09-19- 19"; volume: "114"; issue: "38"; pages: 
            "E8062-E8071"
COMMENT     Sequence Label: pDEST-APIC-Flp1
FEATURES             Location/Qualifiers
     source          1..13143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1940..2325
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(2335..2353)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2805..2876,3278..3551,3626..4280,4342..4657,4861..4992,
                     5063..5677)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(6456..7010,7011..7041)
                     /label=P element 5' end
     rep_origin      complement(7276..7864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7356..7375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8035..8826,8827..8895))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8658..8677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8896..9000)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9068..9086
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9124..9146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9246..9265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    9474..9543
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(10087..10117,10118..10319)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    10717..11102
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    11136..11260
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(11285..11290,11291..11308,11309..11315)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             11369..12028
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11435..11454)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             12370..12675
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     12554..12573
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(12716..12840)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgccgcagt ttgatatcct ctgcaagacc ccaccaaagg tgttggtgcg tcaattcgtg
       61 gagcgatttg agaggccgtc gggtgagaag atcgccctgt gcgctgccga gttgacctat
      121 ttgtgttgga tgatcactca taatggcacc gcgattaagc gcgctacctt tatgagctat
      181 aacactatca ttagcaattc cctgtccttt gacatagtaa acaagtccct gcagtttaaa
      241 tacaagactc agaaggccac tatattggag gcttcgctga aaaagttgat cccggcatgg
      301 gagttcacga tcatcccata ctacggtcag aaacaccaga gcgatattac cgatattgta
      361 agcagcctcc agctgcagtt tgagtccagc gaagaggctg ataagggtaa tagtcacagc
      421 aaaaagatgc tgaaggcact gctgtccgag ggcgaaagca tctgggagat tactgagaaa
      481 atcctgaact cgtttgagta caccagccga ttcaccaaga cgaagaccct gtaccagttc
      541 ctctttttgg caaccttcat caattgtggt cgcttcagtg acatcaaaaa cgtggaccct
      601 aaatcgttca agctggtgca gaataagtac ctgggtgtta tcatccagtg cttggttacc
      661 gagactaaga cctccgtgtc caggcacatt tatttcttct ccgcaagggg tcgcatcgat
      721 ccgctggtat acttggatga gttccttcgg aatagtgaac cagtcttgaa gcgcgtgaac
      781 aggacgggca attccagtag caacaagcaa gagtaccagc tgctgaagga taatcttgtt
      841 cggtcgtaca acaaagcctt gaagaaaaat gcgccatact cgatctttgc aatcaagaat
      901 ggtcccaaga gtcatattgg ccggcacctg atgaccagtt tcttgtccat gaagggtctg
      961 actgagctca caaacgtcgt tggcaactgg tcggacaagc gagcgagtgc cgtcgcccgc
     1021 acgacataca cccaccagat cacggccata ccagatcact atttcgcgct ggtgtcgcgt
     1081 tattatgcct atgatcccat cagcaaggag atgatcgcgc tgaaggacga aaccaatcca
     1141 atcgaggagt ggcagcatat cgagcaactt aagggaagcg ctgagggtag catccgttac
     1201 cccgcctgga acggcatcat cagccaggag gttctggatt acctgagctc ctacatcaat
     1261 cgccgtattt aatctagaat taattaacta agccagtcgg caatcggacg ccttcgaaac
     1321 atgcaacact aatgtttaat tcagatttca gcagagacaa gctaaaacac cgacgagttg
     1381 taatcatttc tgtgcgccag catatatttc ttatatacaa cgtaatacat aattatgtaa
     1441 ttctagcatc tccccaacac tcacatacaa acaaacaaaa aatacaaaca cacaaaacgt
     1501 atttacccgc acgcatcctt ggcgaggttg agtatgaaac aaaaacaaaa cttaatttag
     1561 agcaaagtaa ttacacgaat aaatttaata aaaaaaaact ataataaaaa cgccaatcat
     1621 gttatttcca aaaaaaaaaa aaaaaaaaaa actagacgag atgtttgcgc ttgtgtcgat
     1681 gaatgtaaag aattgcaact acatagatgg taattaattt cagctgtaag aagtacaagt
     1741 ttttcataat tgtgagaaaa gtgcagtagt atcgcttaaa gttcagtttt aatcagggca
     1801 tttagcagct cgaacccaat gtatttgtaa gcccaataaa cgtgtaaatg cctagagcaa
     1861 aatatcacaa ctgtagcttt tcatccgaaa gataataatg aatagactga cctgacgttt
     1921 ttcaaaatgc gtaactagtc acgtaataag tgtgcgttga atttattcgc aaaaacattg
     1981 catattttcg gcaaagtaaa attttgttgc ataccttatc aaaaaataag tgctgcatac
     2041 tttttagaga aaccaaataa ttttttattg catacccgtt tttaataaaa tacattgcat
     2101 accctctttt aataaaaaat attgcatact ttgacgaaac aaattttcgt tgcataccca
     2161 ataaaagatt attatattgc atacccgttt ttaataaaat acattgcata ccctctttta
     2221 ataaaaaata ttgcatacgt tgacgaaaca aattttcgtt gcatacccaa taaaagatta
     2281 ttatattgca taccttttct tgccatacca tttagccgat caattctagt atgtatgtaa
     2341 gttaataaaa cccatttttg cggaaagtag ataaaaaaaa catttttttt ttttactgca
     2401 ctggatatca ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa gggtatttga
     2461 tgtaccaggt tctttcgatt acctctcact caaaatgaca ttccactcaa agtcagcgct
     2521 gtttgcctcc ttctctgtcc acagaaatat cgccgtctct ttcgccgctg cgtccgctat
     2581 ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt ccgccttcag ttgcattttg
     2641 tcagcggttt cgtgacgaag ctccaagcgg tttacgccat caattaaaca caaagtgctg
     2701 tgccaaaact cctctcgctt cttatttttg tttgtttttt gagtgattgg ggtggtgatt
     2761 ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc
     2821 aggagctatt aattcgcgga ggcagcaaac acccatctgc cgagcatctg aacaatgtga
     2881 gtagtacatg tgcatacatc ttaagttcac ttgatctata ggaactgcga ttgcaacatc
     2941 aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc caaaccgcaa ttgcacaaac
     3001 aaatagtgac acgaaacaga ttattctggt agctgttctc gctatataag acaatttttg
     3061 agatcatatc atgatcaaga catctaaagg cattcatttt cgactatatt cttttttaca
     3121 aaaaatataa caaccagata ttttaagctg atcctagatg cacaaaaaat aaataaaagt
     3181 ataaacctac ttcgtaggat acttcggggt actttttgtt cggggttaga tgagcataac
     3241 gcttgtagtt gatatttgag atcccctatc attgcagggt gacagcggag cggcttcgca
     3301 gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc ggccacccag
     3361 tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca cctatgcctg
     3421 gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc ggcagctggt
     3481 caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca ggaaacattt
     3541 gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc ttaataaagg
     3601 gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga acttttggcc
     3661 gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc ctttcgatcg
     3721 ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca acctgtggac
     3781 gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt tatcggctcc
     3841 ctaacggcca gggaacacct gattttccaa gccatggtgc ggatgccacg acatctgacc
     3901 tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct cagcaaatgt
     3961 cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag
     4021 cgtctggcat tcgcctccga ggctctaacc gatccgccgc ttctgatctg cgatgagccc
     4081 acctccggac tggactcctt taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg
     4141 cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct gtttgagctc
     4201 tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac tcccagcgaa
     4261 gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct agtattacat
     4321 aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca atccggcgga
     4381 cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc gtgatcggat
     4441 cgccaagata tgcgacaatt ttgccattag caaagtagcc cgggatatgg agcagttgtt
     4501 ggccaccaaa aatctggaga agccactgga gcagccggag aatgggtaca cctacaaggc
     4561 cacctggttc atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga
     4621 accactcctc gtaaaagtgc gacttattca gacaacggtg agtggttcca gtggaaacaa
     4681 atgatataac gcttacaatt cttggaaaca aattcgctag attttagata gaattgcctg
     4741 attccacacc cttcttagtt tttttcaatg agatgtatag tttatagttt tgcagaagat
     4801 aaataaattt catttaactc gcgaatatta atgagatgcg agtaacattt taatttgcag
     4861 atggttgcca tcttgattgg cctcatcttt ttgggccaac aactcacgca agtgggtgtg
     4921 atgaatatca acggagccat cttcctcttc ctgaccaaca tgacctttca aaacgtcttt
     4981 gccacgataa atgtaagtca tgtttagaat acatttgcat ttcaataatt tactaacttt
     5041 ctaatgaatc gattcgattt aggtgttcac ctcagagctg ccagttttta tgagggaggc
     5101 ccgaagtcga ctttatcgct gtgacacata ctttctgggc aaaacgattg ccgaattgcc
     5161 gctttttctc acagtgccac tggtcttcac ggcgattgcc tatccgatga tcggactgcg
     5221 ggccggagtg ctgcacttct tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc
     5281 aacgtccttc ggatatctaa tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt
     5341 gggtccgccg gttatcatac cattcctgct ctttggcggc ttcttcttga actcgggctc
     5401 ggtgccagta tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg
     5461 tctgctgatt aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa
     5521 caccacgtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga
     5581 tctgccgctg gactacgtgg gtctggccat tctcatcgtg agcttccggg tgctcgcata
     5641 tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc gaaataactg
     5701 cttgtttttt ttttttacca ttattaccat cgtgtttact gtttattgcc ccctcaaaaa
     5761 gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa tacaagtatt
     5821 tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa aagacttaca
     5881 cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg ggatatattt
     5941 tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg
     6001 tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc accttttgtt
     6061 cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggttcgctca aatggttccg
     6121 agtggttcat ttcgtctcaa tagaaattag taataaatat ttgtatgtac aatttatttg
     6181 ctccaatata tttgtatata tttccctcac agctatattt attctaattt aatattatga
     6241 ctttttaagg taattttttg tgacctgttc ggagtgatta gcgttacaat ttgaactgaa
     6301 agtgacatcc agtgtttgtt ccttgtgtag atgcatctca aaaaaatggt gggcataata
     6361 gtgttgttta tatatatcaa aaataacaac tataataata agaatacatt taatttagaa
     6421 aatgcttgga tttcactgga actagaatta attcggctgc tgctctaaac gacgcatttc
     6481 gtactccaaa gtacgaattt tttccctcaa gctcttattt tcattaaaca atgaacagga
     6541 cctaacgcac agtcacgtta ttgtttacat aaatgatttt ttttactatt caaacttact
     6601 ctgtttgtgt actcccactg gtatagcctt cttttatctt ttctggttca ggctctatca
     6661 ctttactagg tacggcatct gcgttgagtc gcctcctttt aaatgtctga ccttttgcag
     6721 gtgcagcctt ccactgcgaa tcattaaagt gggtatcaca aatttgggag ttttcaccaa
     6781 ggctgcaccc aaggctctgc tcccacaatt ttctcttaat agcacacttc ggcacgtgaa
     6841 ttaattttac tccagtcaca gctttgcagc aaaatttgca atatttcatt tttttttatt
     6901 ccacgtaagg gttaatgttt tcaaaaaaaa attcgtccgc acacaacctt tcctctcaac
     6961 aagcaaacgt gcactgaatt taagtgtata cttcggtaag cttcggctat cgacgggacc
     7021 accttatgtt atttcatcat gggccagacc cacgtagtcc agcggcagat cggcggcgga
     7081 gaagttaagc gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt
     7141 gcagctaatt tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc
     7201 gtaacggaac catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt
     7261 caagaagaag gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7441 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7681 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7741 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7801 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7861 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7921 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7981 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8041 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8101 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8161 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8221 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8281 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8341 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8401 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8461 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8521 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8581 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8641 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8701 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8761 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8821 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8881 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8941 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9001 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     9061 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     9121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     9181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     9241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat tcgacatgcc cgccgtgacc
     9301 gtcgagaacc cgctgacgct gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc
     9361 gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc
     9421 gcgttcgccg ggatcaacta ccgccacctc gacccgttca tcatgatgga ccagatgggt
     9481 gaggtggagt acgcgcccgg ggagcccaag ggcacgccct ggcacccgca ccgcggcttc
     9541 gagaccgtga cctacatcgt cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac
     9601 taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg
     9661 gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg
     9721 cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg
     9781 agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt
     9841 ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt
     9901 attggcacaa atataattac attagctttt tgagggggca ataaacagta aacacgatgg
     9961 taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc
    10021 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg
    10081 ccagaccatg atgaaataac ataaggtggt cccgtcggca agagacatcc acttaacgta
    10141 tgcttgcaat aagtgcgagt gaaaggaata gtattctgag tgtcgtattg agtctgagtg
    10201 agacagcgat atgattgttg attaaccctt agcatgtccg tggggtttga attaactcat
    10261 aatattaatt agacgaaatt atttttaaag ttttattttt aataatttgc gagtacgcaa
    10321 agcttcctag gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
    10381 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
    10441 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    10501 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    10561 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    10621 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
    10681 cgcgccagcc gaactgttcg ccaagcttgg ctgcatcacg taataagtgt gcgttgaatt
    10741 tattcgcaaa aacattgcat attttcggca aagtaaaatt ttgttgcata ccttatcaaa
    10801 aaataagtgc tgcatacttt ttagagaaac caaataattt tttattgcat acccgttttt
    10861 aataaaatac attgcatacc ctcttttaat aaaaaatatt gcatactttg acgaaacaaa
    10921 ttttcgttgc atacccaata aaagattatt atattgcata cccgttttta ataaaataca
    10981 ttgcataccc tcttttaata aaaaatattg catacgttga cgaaacaaat tttcgttgca
    11041 tacccaataa aagattatta tattgcatac cttttcttgc cataccattt agccgatcaa
    11101 ttgtgctcgg caacagcatg ctgcagcaga tcatcacaag tttgtacaaa aaagctgaac
    11161 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
    11221 ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc
    11281 aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc
    11341 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
    11401 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
    11461 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
    11521 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
    11581 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
    11641 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
    11701 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
    11761 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
    11821 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
    11881 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
    11941 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
    12001 ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata
    12061 acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac
    12121 ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc
    12181 gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa
    12241 ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag
    12301 ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg
    12361 gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
    12421 gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc
    12481 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
    12541 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
    12601 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
    12661 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag
    12721 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
    12781 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt
    12841 gatgatctgg tctagcgagc gccggagtat aaatagaggc gcttcgtcta cggagcgaca
    12901 attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag
    12961 cgcagctgag caagctaaac aatcgtaaag tgcaagttaa agtgaatcaa ttaaaagtaa
    13021 ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc cggtgagatt gtacaccctt
    13081 cttaaagatc cccttcaaag tctaaattct tccttttcgt ttagccaaga ctcgagaatc
    13141 aaa
//
