LOCUS       sequence-243308-       14616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gateway cloning destination vector for expressing enhancer driven 
            CD4-IFP2.0-T2A-HO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14616)
  AUTHORS   Poe AR, Wang B, Sapar ML, Ji H, Li K, Onabajo T, Fazliyeva R, Gibbs 
            M, Qiu Y, Hu Y, Han C
  TITLE     Robust CRISPR/Cas9-Mediated Tissue Specific Mutagenesis Reveals Gene
            Redundancy and Perdurance in Drosophila.
  JOURNAL   Genetics. 2018 Nov 30. pii: genetics.118.301736. doi: 
            10.1534/genetics.118.301736.
  PUBMED    30504366
REFERENCE   2  (bases 1 to 14616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2018 Nov 30. pii: genetics.118.301736. doi: 
            10.1534/genetics.118.301736."
COMMENT     Sequence Label: pDEST-HemmarI2
FEATURES             Location/Qualifiers
     source          1..14616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1282..2244
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLD
                     WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
                     VRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     CDS             2248..2259
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2251..2304
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    3840..4225
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(4235..4253)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4705..4776,5178..5451,5526..6180,6242..6557,6761..6892,
                     6963..7577)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8356..8910,8911..8941)
                     /label=P element 5' end
     rep_origin      complement(9176..9764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9256..9275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9935..10726,10727..10795))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10558..10577
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10796..10900)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10968..10986
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11024..11046)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11146..11165
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    11374..11443
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(11987..12017,12018..12219)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    12617..13002
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    13037..13161
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(13186..13191,13192..13209,13210..13216)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             13270..13929
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(13336..13355)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             14271..14576
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     14455..14474
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
      121 gtggtgattt gtcaaggtac cgagcgccgg agtataaata gaggcgcttc gtctacggag
      181 ctcagttgtg aatgaatgtt cgagccgagc agacgtgccg cgcgaaagct aagcaaataa
      241 acaagcgcag ctgagcaagc taaacaatcg taaagtgcaa gttaaagtga atcaattaaa
      301 agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccggtg agattgtaca
      361 cccttcttaa agatcccctt caaagtctaa attcttcctt ttcgtttagc cactcgagag
      421 aaaaccggta cgcgtgaatt caacttaaaa aaaaaaatca aaatgaatcc caagagcgaa
      481 gtcctcattg cagccgtgct gttcatgctg ctggcctgcg tccagtgtca agctagcggt
      541 ggcggcggaa gtggaggtgg aggctcgttc cagaaggcct ccagcatagt ctataagaaa
      601 gagggggaac aggtggagtt ctccttccca ctcgccttta cagttgaaaa gctgacgggc
      661 agtggcgagc tgtggtggca ggcggagagg gcttcctcct ccaagtcttg gatcaccttt
      721 gacctgaaga acaaggaagt gtctgtaaaa cgggttaccc aggaccctaa gctccagatg
      781 ggcaagaagc tcccgctcca cctcaccctg ccccaggcct tgcctcagta tgctggctct
      841 ggaaacctca ccctggccct tgaagcgaaa acaggaaagt tgcatcagga agtgaacctg
      901 gtggtgatga gagccactca gctccagaaa aatttgacct gtgaggtgtg gggacccacc
      961 tcccctaagc tgatgctgag cttgaaactg gagaacaagg aggcaaaggt ctcgaagcgg
     1021 gagaaggcgg tgtgggtgct gaaccctgag gcggggatgt ggcagtgtct gctgagtgac
     1081 tcgggacagg tcctgctgga atccaacatc aaggttctgc ccacatggtc caccccggtg
     1141 cagccaatgg ccctgattgt gctggggggc gtcgccggcc tcctgctttt cattgggcta
     1201 ggcatcttct tctgtgtcag gtgccggcac cgaaggcgcg gcggaagcgg aggcggatcc
     1261 atggtgagca agggcgagga gatggctcgg gaccctcaac ctttcttccc tcctctgtat
     1321 ctcggaggac cagagatcac tactgagaac tgcgagcggg aacctataca tataccaggt
     1381 tctattcaac ctcatggagc actgctcaca gccgatggac attctggcga ggtgctgcaa
     1441 gtttcactca acgcagccac tttcctgggc caggaaccaa ctgttctgcg gggacagacc
     1501 ctggcagccc tgctgccaga ccagtggcct gctctgcaga ctgccctgcc tccaggctgc
     1561 caggacgccc tgcagtatcg cgccaccctg gattggcccg ccgccggaca cctgagcctg
     1621 accgtgcata gggtggccga gctgctgatt ctggaattcg agcctactga ggcatgggac
     1681 tctattggac cccacgctct gagaaatgca atgttcgctc tggagagtgc tcctaatctc
     1741 cgggcactgg ctgaagtcgc aacccaaaca gtccgggaac tgtcaggttt cgaccgggtg
     1801 atgctgtaca agtttgcacc agacgcaaca ggagaggtta ttgccgaagc aaggcgcgag
     1861 ggcatgcagg cttacctcgg gcataggttt cccgcatcca ccacccctgc acaagctagg
     1921 gccctctaca caagacacct gctccggctg accgcagaca ccagggctgc agcagtgccc
     1981 ctcgaccccg tgctgaatcc ccagacaaat gctcctacac ctctgggcgg agctgtcctc
     2041 agagctacat ccccaatgca catgcagtac ctgaggaata tgggagtggg ctcctccctg
     2101 agcgtcagcg tcgtggtcgg cggccagctg tggggactga ttgtctgcca ccatcagaca
     2161 ccctacgtgc tgccaccaga tctgcggacc accctggagt atctggggag gctcctgtcc
     2221 ctgcaggtgc agaggaaaga agccgatatc gagggcagag gaagtctgct aacatgcggt
     2281 gacgtcgagg agaatcctgg cccaatggaa agaccacagc cagactctat gccacaggat
     2341 ctgagcgaag ccctcaagga ggccaccaag gaagtgcaca cccaggccga gaacgctgag
     2401 ttcatgagaa acttccagaa aggacaggtt actcgcgacg gattcaagct ggtgatggct
     2461 agcctctatc acatctacgt ggccctggag gaggaaattg agagaaacaa ggaatccccc
     2521 gtgttcgctc cagtttattt cccagaggag ctgcatcgga aggccgccct ggaacaggat
     2581 ctggcttttt ggtatggccc ccgctggcag gaggtcattc cttatacccc cgctatgcaa
     2641 cgctacgtta agagactcca cgaggtgggc cgcacagaac ctgagctgct cgtggcccat
     2701 gcctacaccc gctacctggg cgacctgtca gggggtcaag ttctgaaaaa gatagcccaa
     2761 aaggccctgg acctgccttc ctctggcgag gggctggcct tctttacttt ccctaacatt
     2821 gcctcagcca caaagttcaa gcagctctac cggtccagga tgaacagcct ggaaatgacc
     2881 ccagccgtgc ggcagcgggt catagaagaa gccaagactg cctttctgct gaatatccag
     2941 ctgtttgagg aactccagga actgctgacc catgacacta aagaccagtc cccctccagg
     3001 gccccaggac tcagacagag ggcctcaaac aaggttcagg actcagcccc cgtggaaaca
     3061 cccaggggaa aaccccctct caatacaaga tctcaagcac ccctgctgag atgggtcctc
     3121 accctgagct tcctcgtcgc taccgttgca gtggggctct atgctatgtg atctagaatt
     3181 aattaactaa gccagtcggc aatcggacgc cttcgaaaca tgcaacacta atgtttaatt
     3241 cagatttcag cagagacaag ctaaaacacc gacgagttgt aatcatttct gtgcgccagc
     3301 atatatttct tatatacaac gtaatacata attatgtaat tctagcatct ccccaacact
     3361 cacatacaaa caaacaaaaa atacaaacac acaaaacgta tttacccgca cgcatccttg
     3421 gcgaggttga gtatgaaaca aaaacaaaac ttaatttaga gcaaagtaat tacacgaata
     3481 aatttaataa aaaaaaacta taataaaaac gccaatcatg ttatttccaa aaaaaaaaaa
     3541 aaaaaaaaaa nctagacgag atgtttgcgc ttgtgtcgat gaatgtaaag aattgcaact
     3601 acatagatgg taattaattt cagctgtaag aagtacaagt ttttcataat tgtgagaaaa
     3661 gtgcagtagt atcgcttaaa gttcagtttt aatcagggca tttagcagct cgaacccaat
     3721 gtatttgtaa gcccaataaa cgtgtaaatg cctagagcaa aatatcacaa ctgtagcttt
     3781 tcatccgaaa gataataatg aatagactga cctgacgttt ttcaaaatgc gtaactagtc
     3841 acgtaataag tgtgcgttga atttattcgc aaaaacattg catattttcg gcaaagtaaa
     3901 attttgttgc ataccttatc aaaaaataag tgctgcatac tttttagaga aaccaaataa
     3961 ttttttattg catacccgtt tttaataaaa tacattgcat accctctttt aataaaaaat
     4021 attgcatact ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     4081 atacccgttt ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatacgt
     4141 tgacgaaaca aattttcgtt gcatacccaa taaaagatta ttatattgca taccttttct
     4201 tgccatacca tttagccgat caattctagt atgtatgtaa gttaataaaa cccatttttg
     4261 cggaaagtag ataaaaaaaa catttttttt ttttactgca ctggatatca ttgaacttat
     4321 ctgatcagtt ttaaatttac ttcgatccaa gggtatttga tgtaccaggt tctttcgatt
     4381 acctctcact caaaatgaca ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc
     4441 acagaaatat cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc accgtttgta
     4501 gcgttacgta gcgtcaatgt ccgccttcag ttgcattttg tcagcggttt cgtgacgaag
     4561 ctccaagcgg tttacgccat caattaaaca caaagtgctg tgccaaaact cctctcgctt
     4621 cttatttttg tttgtttttt gagtgattgg ggtggtgatt ggttttgggt gggtaagcag
     4681 gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc aggagctatt aattcgcgga
     4741 ggcagcaaac acccatctgc cgagcatctg aacaatgtga gtagtacatg tgcatacatc
     4801 ttaagttcac ttgatctata ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga
     4861 actgcgaccc acaaaaatcc caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga
     4921 ttattctggt agctgttctc gctatataag acaatttttg agatcatatc atgatcaaga
     4981 catctaaagg cattcatttt cgactatatt cttttttaca aaaaatataa caaccagata
     5041 ttttaagctg atcctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat
     5101 acttcggggt actttttgtt cggggttaga tgagcataac gcttgtagtt gatatttgag
     5161 atcccctatc attgcagggt gacagcggag cggcttcgca gagctgcatt aaccagggct
     5221 tcgggcaggc caaaaactac ggcacgctcc ggccacccag tccgccggag gactccggtt
     5281 cagggagcgg ccaactagcc gagaacctca cctatgcctg gcacaatatg gacatctttg
     5341 gggcggtcaa tcagccgggc tccggatggc ggcagctggt caaccggaca cgcggactat
     5401 tctgcaacga gcgacacata ccggcgccca ggaaacattt gctcaagaac ggtgagtttc
     5461 tattcgcagt cggctgatct gtgtgaaatc ttaataaagg gtccaattac caatttgaaa
     5521 ctcagtttgc ggcgtggcct atccgggcga acttttggcc gtgatgggca gttccggtgc
     5581 cggaaagacg accctgctga atgcccttgc ctttcgatcg ccgcagggca tccaagtatc
     5641 gccatccggg atgcgactgc tcaatggcca acctgtggac gccaaggaga tgcaggccag
     5701 gtgcgcctat gtccagcagg atgacctctt tatcggctcc ctaacggcca gggaacacct
     5761 gattttccaa gccatggtgc ggatgccacg acatctgacc tatcggcagc gagtggcccg
     5821 cgtggatcag gtgatccagg agctttcgct cagcaaatgt cagcacacga tcatcggtgt
     5881 gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag cgtctggcat tcgcctccga
     5941 ggctctaacc gatccgccgc ttctgatctg cgatgagccc acctccggac tggactcctt
     6001 taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg cagaagggca agaccgtcat
     6061 cctgaccatt catcagccgt cttccgagct gtttgagctc tttgacaaga tccttctgat
     6121 ggccgagggc agggtagctt tcttgggcac tcccagcgaa gccgtcgact tcttttccta
     6181 gtgagttcga tgtgtttatt aagggtatct agtattacat aacatctcaa ctcctatcca
     6241 gcgtgggtgc ccagtgtcct accaactaca atccggcgga cttttacgta caggtgttgg
     6301 ccgttgtgcc cggacgggag atcgagtccc gtgatcggat cgccaagata tgcgacaatt
     6361 ttgccattag caaagtagcc cgggatatgg agcagttgtt ggccaccaaa aatctggaga
     6421 agccactgga gcagccggag aatgggtaca cctacaaggc cacctggttc atgcagttcc
     6481 gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga accactcctc gtaaaagtgc
     6541 gacttattca gacaacggtg agtggttcca gtggaaacaa atgatataac gcttacaatt
     6601 cttggaaaca aattcgctag attttagata gaattgcctg attccacacc cttcttagtt
     6661 tttttcaatg agatgtatag tttatagttt tgcagaagat aaataaattt catttaactc
     6721 gcgaatatta atgagatgcg agtaacattt taatttgcag atggttgcca tcttgattgg
     6781 cctcatcttt ttgggccaac aactcacgca agtgggtgtg atgaatatca acggagccat
     6841 cttcctcttc ctgaccaaca tgacctttca aaacgtcttt gccacgataa atgtaagtca
     6901 tgtttagaat acatttgcat ttcaataatt tactaacttt ctaatgaatc gattcgattt
     6961 aggtgttcac ctcagagctg ccagttttta tgagggaggc ccgaagtcga ctttatcgct
     7021 gtgacacata ctttctgggc aaaacgattg ccgaattgcc gctttttctc acagtgccac
     7081 tggtcttcac ggcgattgcc tatccgatga tcggactgcg ggccggagtg ctgcacttct
     7141 tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc aacgtccttc ggatatctaa
     7201 tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt gggtccgccg gttatcatac
     7261 cattcctgct ctttggcggc ttcttcttga actcgggctc ggtgccagta tacctcaaat
     7321 ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg tctgctgatt aaccaatggg
     7381 cggacgtgga gccgggcgaa attagctgca catcgtcgaa caccacgtgc cccagttcgg
     7441 gcaaggtcat cctggagacg cttaacttct ccgccgccga tctgccgctg gactacgtgg
     7501 gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg
     7561 cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt ttttttacca
     7621 ttattaccat cgtgtttact gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt
     7681 gccaataaaa acaagatatg acctatagaa tacaagtatt tccccttcga acatccccac
     7741 aagtagactt tggatttgtc ttctaaccaa aagacttaca cacctgcata ccttacatca
     7801 aaaactcgtt tatcgctaca taaaacaccg ggatatattt tttatataca tacttttcaa
     7861 atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg tttcgtagtt gctctttcgc
     7921 tgtctcccac ccgctctccg caacacattc accttttgtt cgacgacctt ggagcgactg
     7981 tcgttagttc cgcgcgattc ggttcgctca aatggttccg agtggttcat ttcgtctcaa
     8041 tagaaattag taataaatat ttgtatgtac aatttatttg ctccaatata tttgtatata
     8101 tttccctcac agctatattt attctaattt aatattatga ctttttaagg taattttttg
     8161 tgacctgttc ggagtgatta gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt
     8221 ccttgtgtag atgcatctca aaaaaatggt gggcataata gtgttgttta tatatatcaa
     8281 aaataacaac tataataata agaatacatt taatttagaa aatgcttgga tttcactgga
     8341 actagaatta attcggctgc tgctctaaac gacgcatttc gtactccaaa gtacgaattt
     8401 tttccctcaa gctcttattt tcattaaaca atgaacagga cctaacgcac agtcacgtta
     8461 ttgtttacat aaatgatttt ttttactatt caaacttact ctgtttgtgt actcccactg
     8521 gtatagcctt cttttatctt ttctggttca ggctctatca ctttactagg tacggcatct
     8581 gcgttgagtc gcctcctttt aaatgtctga ccttttgcag gtgcagcctt ccactgcgaa
     8641 tcattaaagt gggtatcaca aatttgggag ttttcaccaa ggctgcaccc aaggctctgc
     8701 tcccacaatt ttctcttaat agcacacttc ggcacgtgaa ttaattttac tccagtcaca
     8761 gctttgcagc aaaatttgca atatttcatt tttttttatt ccacgtaagg gttaatgttt
     8821 tcaaaaaaaa attcgtccgc acacaacctt tcctctcaac aagcaaacgt gcactgaatt
     8881 taagtgtata cttcggtaag cttcggctat cgacgggacc accttatgtt atttcatcat
     8941 gggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc gtctccagga
     9001 tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct
     9061 ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac catgagaggt
     9121 acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag gcgtttttcc
     9181 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9241 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9301 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9361 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9421 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9481 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9541 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9601 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9661 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9721 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9781 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9841 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9901 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9961 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    10021 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    10081 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    10141 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    10201 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10261 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10321 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10381 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10441 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10501 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10561 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10621 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10681 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10741 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10801 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10861 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10921 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    10981 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    11041 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    11101 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    11161 ttgtactgag agtgcaccat tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct
    11221 gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc
    11281 gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta
    11341 ccgccacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg
    11401 ggagcccaag ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt
    11461 cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
    11521 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
    11581 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
    11641 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
    11701 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
    11761 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
    11821 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
    11881 aacaagcagt tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga
    11941 gccagatatg cgagcacccg gaagctcacg atgagaatgg ccagaccatg atgaaataac
    12001 ataaggtggt cccgtcggca agagacatcc acttaacgta tgcttgcaat aagtgcgagt
    12061 gaaaggaata gtattctgag tgtcgtattg agtctgagtg agacagcgat atgattgttg
    12121 attaaccctt agcatgtccg tggggtttga attaactcat aatattaatt agacgaaatt
    12181 atttttaaag ttttattttt aataatttgc gagtacgcaa agcttcctag gtcaagaccg
    12241 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
    12301 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
    12361 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
    12421 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
    12481 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
    12541 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    12601 ccaagcttgg ctgcatcacg taataagtgt gcgttgaatt tattcgcaaa aacattgcat
    12661 attttcggca aagtaaaatt ttgttgcata ccttatcaaa aaataagtgc tgcatacttt
    12721 ttagagaaac caaataattt tttattgcat acccgttttt aataaaatac attgcatacc
    12781 ctcttttaat aaaaaatatt gcatactttg acgaaacaaa ttttcgttgc atacccaata
    12841 aaagattatt atattgcata cccgttttta ataaaataca ttgcataccc tcttttaata
    12901 aaaaatattg catacgttga cgaaacaaat tttcgttgca tacccaataa aagattatta
    12961 tattgcatac cttttcttgc cataccattt agccgatcaa ttgtgctcgg caacagcatg
    13021 ctgcagaaga cttatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
    13081 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
    13141 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct
    13201 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
    13261 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
    13321 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
    13381 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
    13441 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
    13501 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
    13561 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
    13621 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
    13681 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
    13741 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
    13801 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
    13861 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
    13921 ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
    13981 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
    14041 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
    14101 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
    14161 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
    14221 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
    14281 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
    14341 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
    14401 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
    14461 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
    14521 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
    14581 caggctccct tatacacagc cagtctgcag gtcgac
//
