LOCUS       sequence-243309-        5736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gateway cloning destination vector to express enhancer driven Gal4 
            in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Han C, Wang D, Soba P, Zhu S, Lin X, Jan LY, Jan YN
  TITLE     Integrins regulate repulsion-mediated dendritic patterning of 
            drosophila sensory neurons by restricting dendrites in a 2D space.
  JOURNAL   Neuron. 2012 Jan 12;73(1):64-78. doi: 10.1016/j.neuron.2011.10.036.
  PUBMED    22243747
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.10"; journalName: "Neuron"; date: 
            "2012-01-12- 12"; volume: "73"; issue: "1"; pages: "64-78"
COMMENT     Sequence Label: pDEST-APIGH
FEATURES             Location/Qualifiers
     source          1..5736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             425..3070
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    3738..4123
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    4157..4281
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4306..4311,4312..4329,4330..4336)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4390..5049
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4456..4475)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5391..5696
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5575..5594
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
      121 gtggtgatga tctggtctag cgagcgccgg agtataaata gaggcgcttc gtctacggag
      181 cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa
      241 acaagcgcag ctgagcaagc taaacaatcg taaagtgcaa gttaaagtga atcaattaaa
      301 agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccggtg agattgtaca
      361 cccttcttaa agatcccctt caaagtctaa attcttcctt ttcgtttagc caagactcga
      421 caaaatgaag ctactgtctt ctatcgaaca agcatgcgat atttgccgac ttaaaaagct
      481 caagtgctcc aaagaaaaac cgaagtgcgc caagtgtctg aagaacaact gggagtgtcg
      541 ctactctccc aaaaccaaaa ggtctccgct gactagggca catctgacag aagtggaatc
      601 aaggctagaa agactggaac agctatttct actgattttt cctcgagaag accttgacat
      661 gattttgaaa atggattctt tacaggatat aaaagcattg ttaacaggat tatttgtaca
      721 agataatgtg aataaagatg ccgtcacaga tagattggct tcagtggaga ctgatatgcc
      781 tctaacattg agacagcata gaataagtgc gacatcatca tcggaagaga gtagtaacaa
      841 aggtcaaaga cagttgactg tatcgattga ctcggcagct catcatgata actccacaat
      901 tccgttggat tttatgccca gggatgctct tcatggattt gattggtctg aagaggatga
      961 catgtcggat ggcttgccct tcctgaaaac ggaccccaac aataatgggt tctttggcga
     1021 cggttctctc ttatgtattc ttcgatctat tggctttaaa ccggaaaatt acacgaactc
     1081 taacgttaac aggctcccga ccatgattac ggatagatac acgttggctt ctagatccac
     1141 aacatcccgt ttacttcaaa gttatctcaa taattttcac ccctactgcc ctatcgtgca
     1201 ctcaccgacg ctaatgatgt tgtataataa ccagattgaa atcgcgtcga aggatcaatg
     1261 gcaaatcctt tttaactgca tattagccat tggagcctgg tgtatagagg gggaatctac
     1321 tgatatagat gttttttact atcaaaatgc taaatctcat ttgacgagca aggtcttcga
     1381 gtcaggttcc ataattttgg tgacagccct acatcttctg tcgcgatata cacagtggag
     1441 gcagaaaaca aatactagct ataattttca cagcttttcc ataagaatgg ccatatcatt
     1501 gggcttgaat agggacctcc cctcgtcctt cagtgatagc agcattctgg aacaaagacg
     1561 ccgaatttgg tggtctgtct actcttggga gatccaattg tccctgcttt atggtcgatc
     1621 catccagctt tctcagaata caatctcctt cccttcttct gtggacgatg tgcagcgtac
     1681 cacaacaggt cccaccatat atcatggcat cattgaaaca gcaaggctct tacaagtttt
     1741 cacaaaaatc tatgaactag acaaaacagt aactgcagaa aaaagtccta tatgtgcaaa
     1801 aaaatgcttg atgatttgta atgagattga ggaggtttcg agacaggcac caaagttttt
     1861 acaaatggat atttccacca ccgctctaac caatttgttg aaggaacacc cttggctatc
     1921 ctttacaaga ttcgaactga agtggaaaca gttgtctctt atcatttatg tattaagaga
     1981 ttttttcact aattttaccc agaaaaagtc acaactagaa caggatcaaa atgatcatca
     2041 aagttatgaa gttaaacgat gctccatcat gttaagcgat gcagcacaaa gaactgttat
     2101 gtctgtaagt agctatatgg acaatcataa tgtcacccca tattttgcct ggaattgttc
     2161 ttattacttg ttcaatgcag tcctagtacc cataaagact ctactctcaa actcaaaatc
     2221 gaatgctgag aataacgaga ccgcacaatt attacaacaa attaacactg ttctgatgct
     2281 attaaaaaaa ctggccactt ttaaaatcca gacttgtgaa aaatacattc aagtactgga
     2341 agaggtatgt gcgccgtttc tgttatcaca gtgtgcaatc ccattaccgc atatcagtta
     2401 taacaatagt aatggtagcg ccattaaaaa tattgtcggt tctgcaacta tcgcccaata
     2461 ccctactctt ccggaggaaa atgtcaacaa tatcagtgtt aaatatgttt ctcctggctc
     2521 agtagggcct tcacctgtgc cattgaaatc aggagcaagt ttcagtgatc tagtcaagct
     2581 gttatctaac cgtccaccct ctcgtaactc tccagtgaca ataccaagaa gcacaccttc
     2641 gcatcgctca gtcacgcctt ttctagggca acagcaacag ctgcaatcat tagtgccact
     2701 gaccccgtct gctttgtttg gtggcgccaa ttttaatcaa agtgggaata ttgctgatag
     2761 ctcattgtcc ttcactttca ctaacagtag caacggtccg aacctcataa caactcaaac
     2821 aaattctcaa gcgctttcac aaccaattgc ctcctctaac gttcatgata acttcatgaa
     2881 taatgaaatc acggctagta aaattgatga tggtaataat tcaaaaccac tgtcacctgg
     2941 ttggacggac caaactgcgt ataacgcgtt tggaatcact acagggatgt ttaataccac
     3001 tacaatggat gatgtatata actatctatt cgatgatgaa gataccccac caaacccaaa
     3061 aaaagagtaa ctagaattaa ttaactaagc cagtcggcaa tcggacgcct tcgaaacatg
     3121 caacactaat gtttaattca gatttcagca gagacaagct aaaacaccga cgagttgtaa
     3181 tcatttctgt gcgccagcat atatttctta tatacaacgt aatacataat tatgtaattc
     3241 tagcatctcc ccaacactca catacaaaca aacaaaaaat acaaacacac aaaacgtatt
     3301 tacccgcacg catccttggc gaggttgagt atgaaacaaa aacaaaactt aatttagagc
     3361 aaagtaatta cacgaataaa tttaataaaa aaaaactata ataaaaacgc caatcatgtt
     3421 atttccaaaa aaaaaaaaaa aaaaaaaaac tagacgagat gtttgcgctt gtgtcgatga
     3481 atgtaaagaa ttgcaactac atagatggta attaatttca gctgtaagaa gtacaagttt
     3541 ttcataattg tgagaaaagt gcagtagtat cgcttaaagt tcagttttaa tcagggcatt
     3601 tagcagctcg aacccaatgt atttgtaagc ccaataaacg tgtaaatgcc tagagcaaaa
     3661 tatcacaact gtagcttttc atccgaaaga taataatgaa tagactgacc tgacgttttt
     3721 caaaatgcgt aactagtcac gtaataagtg tgcgttgaat ttattcgcaa aaacattgca
     3781 tattttcggc aaagtaaaat tttgttgcat accttatcaa aaaataagtg ctgcatactt
     3841 tttagagaaa ccaaataatt ttttattgca tacccgtttt taataaaata cattgcatac
     3901 cctcttttaa taaaaaatat tgcatacttt gacgaaacaa attttcgttg catacccaat
     3961 aaaagattat tatattgcat acccgttttt aataaaatac attgcatacc ctcttttaat
     4021 aaaaaatatt gcatacgttg acgaaacaaa ttttcgttgc atacccaata aaagattatt
     4081 atattgcata ccttttcttg ccataccatt tagccgatca attgtgctcg gcaacagcat
     4141 gctgcagcag atcatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     4201 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     4261 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct
     4321 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
     4381 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     4441 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     4501 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     4561 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     4621 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     4681 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     4741 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     4801 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     4861 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     4921 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     4981 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     5041 ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     5101 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     5161 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     5221 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     5281 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     5341 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     5401 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     5461 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     5521 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     5581 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     5641 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     5701 caggctccct tatacacagc cagtctgcag gtcgac
//
