LOCUS       sequence-243314-       14900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express sgRNA and dCas9-KRAB from lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14900)
  AUTHORS   Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM,
            Reddy TE, Crawford GE, Gersbach CA
  TITLE     Highly specific epigenome editing by CRISPR-Cas9 repressors for 
            silencing of distal regulatory elements.
  JOURNAL   Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 
            2015 Oct 26.
  PUBMED    26501517
REFERENCE   2  (bases 1 to 14900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3630"; journalName: "Nat Methods"; date: "2015-12"; 
            volume: "12"; issue: "12"; pages: "1143-9"
COMMENT     Sequence Label: pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
FEATURES             Location/Qualifiers
     source          1..14900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     262..281
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      454..463
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             463..528
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             535..555
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(565..591,592..594,595..3078,3079..3084,3085..3150,
                     3151..3153,3154..4668)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(565..591,592..594,595..3081,3082..3084,3085..4668)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             565..4668
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4681..4701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4735..4929
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             4996..5016
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5023..5076
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5077..5676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5077..5096)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5573..5593
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(5730..5763)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5788..6376
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5841..5861)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6259..6270)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6379..6395)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6380..6396)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6901..7081
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7107..7124)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7113..7337
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7383..7811
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7470..7489)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7680..7701
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7820..7840)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7825..8154
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8005..8140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8067..8086
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8202..8249
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8268..8642
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8772..8893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8809..8828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8863..8882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8942..8958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8942..8958)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8955..8977)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8966..8982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8990..8996,8997..9014,9015..9020))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9035..9056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9173..9190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9344..9932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9424..9443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10103..10894,10895..10963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10726..10745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10964..11068)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11143..11162)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11334..11713
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11715..11913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11868..11888
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11931..12111
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12158..12283
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12776..13009
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13194..13238
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13536..13653
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(13697..13730)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        complement(13741..13816)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(13833..14081)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(13891..13910)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(14061..14081)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        join(14114..14900,1..425)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt
       61 cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg
      121 cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg
      181 cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat
      241 gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga
      301 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
      361 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
      421 tagacgaagc ttgggctgca ggtcgactct agagccacca tggactacaa agaccatgac
      481 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag
      541 aagaagagga aggtgggccg cggaatggac aagaagtact ccattgggct cgccatcggc
      601 acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc
      661 aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg
      721 ttcgactccg gggaaaccgc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat
      781 acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag
      841 gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag
      901 cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac
      961 ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg
     1021 ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg
     1081 gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac
     1141 aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg
     1201 agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag
     1261 aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt
     1321 aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat
     1381 gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg
     1441 gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc
     1501 accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg
     1561 actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc
     1621 gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt
     1681 tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag
     1741 cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac
     1801 cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt
     1861 ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta
     1921 ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc
     1981 atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc
     2041 gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct
     2101 ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa
     2161 gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc
     2221 ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt
     2281 gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga
     2341 acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac
     2401 gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt
     2461 gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag
     2521 aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac
     2581 aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac
     2641 ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa
     2701 gtttctggcc agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct
     2761 atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga
     2821 aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag
     2881 ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg
     2941 tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac
     3001 ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg
     3061 ctctccgact acgacgtgga tgccatcgtg ccccagtctt ttctcaaaga tgattctatt
     3121 gataataaag tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca
     3181 gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc
     3241 acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat
     3301 aaagccggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc
     3361 caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag
     3421 gtgaaagtta ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt
     3481 tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg
     3541 gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac
     3601 tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc
     3661 accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg
     3721 gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc
     3781 gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg
     3841 aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg
     3901 aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc
     3961 ggattcgatt ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg
     4021 aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca
     4081 agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa
     4141 gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga
     4201 atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac
     4261 gttaatttct tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat
     4321 gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa
     4381 ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct
     4441 gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg
     4501 tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac
     4561 agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt
     4621 acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac
     4681 cccaagaaga agaggaaggt ggctagcgat gctaagtcac tgactgcctg gtcccggaca
     4741 ctggtgacct tcaaggatgt gtttgtggac ttcaccaggg aggagtggaa gctgctggac
     4801 actgctcagc agatcctgta cagaaatgtg atgctggaga actataagaa cctggtttcc
     4861 ttgggttatc agcttactaa gccagatgtg atcctccggt tggagaaggg agaagagccc
     4921 tggctggtgg agagagaaat tcaccaagag acccatcctg attcagagac tgcatttgaa
     4981 atcaaatcat cagttccgaa aaagaaacgc aaagttgcta gcgagggcag aggaagtctt
     5041 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     5101 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     5161 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     5221 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     5281 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5341 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     5401 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     5461 ggcgtgtcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     5521 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     5581 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     5641 cgcacctggt gcatgacccg caagcccggt gcctgaccag cacactggcg gccgttacta
     5701 gcttctgcag cacgaccggt tgataataga taacttcgta tagcatacat tatacgaagt
     5761 tatgaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     5821 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5881 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5941 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6001 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6061 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6121 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6181 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6241 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6301 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6361 tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc
     6421 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     6481 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     6541 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6601 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     6661 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     6721 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     6781 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     6841 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     6901 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6961 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7021 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7081 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     7141 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     7201 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     7261 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     7321 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     7381 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7441 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7501 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7561 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7621 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7681 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7741 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7801 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7861 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7921 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7981 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8041 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     8101 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     8161 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     8221 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     8281 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     8341 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     8401 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     8461 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     8521 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     8581 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     8641 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8701 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8761 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8821 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8881 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     8941 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9001 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9061 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9121 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9781 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9961 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10021 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10081 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10141 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10201 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10261 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10321 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10381 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10441 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10501 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10561 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10621 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10681 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10741 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10801 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10861 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10921 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10981 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11041 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    11101 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    11161 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    11221 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
    11281 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
    11341 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
    11401 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
    11461 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
    11521 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
    11581 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
    11641 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
    11701 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
    11761 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
    11821 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
    11881 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
    11941 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    12001 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    12061 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
    12121 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    12181 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    12241 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    12301 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    12361 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    12421 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    12481 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    12541 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    12601 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
    12661 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
    12721 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
    12781 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    12841 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
    12901 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
    12961 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    13021 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
    13081 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
    13141 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
    13201 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    13261 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    13321 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
    13381 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
    13441 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
    13501 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
    13561 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    13621 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
    13681 ccagtttggt taattaataa cttcgtatag catacattat acgaagttat taaaaaaaaa
    13741 gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
    13801 tttctagctc taaaactgag acgtccgtct ccggtgtttc gtcctttcca caagatatat
    13861 aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa
    13921 cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt
    13981 actaatatct ttgtgtttac agtcaaatta attccaatta tctctctaac agccttgtat
    14041 cgtatatgca aatatgaagg aatcatggga aataggccct cttaattaat taacccgtgt
    14101 cggctccaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc
    14161 acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg
    14221 acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag
    14281 aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc
    14341 ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc
    14401 ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag
    14461 ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc
    14521 gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt
    14581 gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg
    14641 gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg
    14701 aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag
    14761 gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc
    14821 atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg
    14881 cgggggcggc agttatggcg
//
