LOCUS       sequence-243315-        6479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW
  TITLE     Optimized large-scale production of high titer lentivirus vector 
            pseudotypes.
  JOURNAL   J Virol Methods. 2004 Dec 15. 122(2):131-9.
  PUBMED    15542136
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol 
            Methods. 2004 Dec 15. 122(2):131-9."
COMMENT     Sequence Label: pHCMV-MokolaG
FEATURES             Location/Qualifiers
     source          1..6479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          696..1268
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1337..1353
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1338..1354
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3088..3107)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3153..3208
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3207..3226)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3566..3582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3566..3582)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3579..3601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3590..3606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3614..3620,3621..3638,3639..3644))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3659..3680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3797..3814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3968..4556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4048..4067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4727..5518,5519..5587))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5350..5369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5588..5692)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5718..6173)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5855..5876)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6067..6086
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6300..6322
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6314..6331
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6315..6331
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6341..6360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6341..6359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gtcgaccgat cctgagaact tcagggtgag tttggggacc cttgattgtt
      721 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
      781 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
      841 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
      901 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
      961 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
     1021 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
     1081 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc
     1141 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     1201 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt
     1261 tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat
     1321 tcctcgacgg atctggtcga ggtcgacggt atcgataagc ttgatcatga atataccttg
     1381 ctttgctgtg atcctcagct tagctactac acattctctg ggagaattcc ccttgtatac
     1441 gattcccgag aaaatagaga aatggacccc catagacatg atccatctta gttgccctaa
     1501 taacatgctg tctgaggaag aaggttgcaa tacagagtct cctttcacct acttcgagct
     1561 caagagtggt tacctagccc atcagaaggt cccaggattt acatgcactg gggttgtgaa
     1621 tgaggcagag acatacacaa actttgtcgg atatgtcacc accaccttca aaaggaagca
     1681 ctttaaacct acagtggctg cttgtcgtga tgcctacaac tggaaagtat caggggaccc
     1741 ccgatatgaa gaatctctac acaccccgta tcccgacagc agctggttaa ggactgtgac
     1801 cacaaccaaa gaagcccttc ttataatatc gccaagcatt gtagagatgg acatatatgg
     1861 caggaccctt cactctccca tgttcccttc ggggaaatgt tccaagctct atccttctgt
     1921 cccctcttgt acaaccaacc atgattacac attgtggttg ccagaagatt ctagtctgag
     1981 tttgatttgc gacatcttca cttccagcag tggacagaag gccatgaatg ggtctcgcat
     2041 ctgcggattc aaggatgaaa ggggatttta cagatccttg aagggatcct gtaagctgac
     2101 attgtgcggg aaacctggaa ttaggctgtt cgacggaact tgggtctctt ttacaaagcc
     2161 ggacgttcat gtgtggtgca ctcccaacca gttagtcaat atacataacg acagactaga
     2221 tgaggttgaa catctgatcg tggacgatat catcaagaag agagaggagt gtttagacac
     2281 gctggaaact atacttatgt ctcaatcagt tagttttaga cggttgagcc atttcagaaa
     2341 gttagttcca ggatatggaa aagcttacac tattttgaac ggcagcttaa tggaaacaaa
     2401 tgtctactac aaaagagttg acaggtgggc ggacattttg ccttctaggg gatgtctgaa
     2461 agtcggacaa cagtgcatgg accctgtcaa aggggtcctc ttcaacggaa ttatcaaggg
     2521 tccggatgga caaatattga ttccagagat gcagtcagag cagctcaaac agcatatgga
     2581 tctgttgaaa gcagctatgt ttcctctccg tcatccttta atcaacagag aggcagtctt
     2641 caagaaggat ggaaatgccg atgattttgt tgatctccat atgcctgatg ttcaaaaatc
     2701 tgtgtcggat gtcgacctgg gcctgcctca ttgggggttc tggttgttag tcggggcaac
     2761 agtagtagcc tttgtggtct tggcgtgctt gctccgtgta tgttgtagga gaatgagaag
     2821 gagaaggtca ctgcgtgcca ctcaggatat ccccctcagc gttgcccctg cccctgtccc
     2881 tcgtgccaaa gtggtgtcat catgggagtc ttctaaaggg ctcccaggta cttgatgcct
     2941 ttatcatact gcctgacaca tagatcacaa cctgattacc tgcctcggca ccagatccgt
     3001 cgaggaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     3061 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     3121 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     3181 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     3241 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     3301 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     3361 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     3421 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     3481 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     3541 cggatcggcc gcaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     3601 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3661 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3721 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3781 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3841 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3901 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3961 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4021 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4081 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4141 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4201 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4261 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4321 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4381 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4441 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4501 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4561 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4621 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4681 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4741 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4801 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4861 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4921 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4981 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5041 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5101 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5161 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5221 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5281 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5341 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5401 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5461 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5521 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5581 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5641 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5701 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     5761 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     5821 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     5881 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     5941 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     6001 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     6061 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     6121 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     6181 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     6241 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     6301 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     6361 cgaattggag ctccaccgcg gtggcggccg ctctagagag cttggcccat tgcatacgtt
     6421 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgtt
//
