LOCUS       sequence-243324-        3068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""Minimal cargo"" vector. Allows introducing any part using BsmBI; 
            but doesn't have cloning reporter. Can be used to generate new 
            promoter/terminator acceptor vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-min2
FEATURES             Location/Qualifiers
     source          1..3068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    82..102
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      136..230
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        303..394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(395..463,464..1255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1259..1298
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1445..1553
                     /note="incP origin of transfer"
     rep_origin      1589..2177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2078..2097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2331..2348
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2363..2503
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2429..2448)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2589..2611
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2605..2796)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(2976..3062)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtcttgaga cgtctctgac ctactagtag cggccgctgc agggagttgt cttcgaagac
      121 ttcgctctag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      181 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
      241 ccaataatta cgatttaaat tagtagcccg cctaatgagc gggctttttt ttaattcccc
      301 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      361 ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc
      421 gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt
      481 gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct
      541 gaacagcggc aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac
      601 ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact
      661 gggccgtcgt attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa
      721 acatctgacc gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga
      781 taacaccgcg gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt
      841 tctgcataac atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga
      901 agcgattccg aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg
      961 taaactgctg acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat
     1021 ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat
     1081 tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc
     1141 ggatggtaaa ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga
     1201 tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga
     1261 tacaattaaa ggctcctttt ggagcctttt tttttggacg acccttgtcc ttttccgctg
     1321 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
     1381 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
     1441 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
     1501 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg
     1561 gccccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1621 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1681 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1741 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1801 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1861 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1921 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1981 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2041 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2101 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2161 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2221 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2281 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2341 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     2401 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2461 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     2521 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     2581 gacagtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     2641 gcagctgcgg taaagctcat cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc
     2701 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     2761 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt
     2821 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     2881 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     2941 gcggcggggg cgcgcccagc tgtctagggc ggcggatttg tcctactcag gagagcgttc
     3001 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     3061 tgatgcct
//
