LOCUS       sequence-243329-        3819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP 
            cloning reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 95 to 129)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP29-1B(sfGFP)
FEATURES             Location/Qualifiers
     source          1..3819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        95..129
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             134..154
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             171..881
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    934..954
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      988..1082
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1203..1222)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1214..2029
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2196..2304
                     /note="incP origin of transfer"
     rep_origin      2340..2928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2829..2848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3082..3099
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3114..3254
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3180..3199)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3340..3362
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3356..3547)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3727..3813)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagagcg tctctaatgg gagtgagacc ggagttgacg gctagctcag tcctaggtac
      121 agtgctagcg tacttgttta actttaagaa ggagatatac aatggtagcc cgtaaaggcg
      181 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
      241 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
      301 agttcatctg tactactggt aaactgccgg taccttggcc gactctggta acgacgctga
      361 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
      421 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
      481 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
      541 tgaaaggcat tgactttaaa gaagatggca atatcctggg ccataagctg gaatacaatt
      601 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
      661 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
      721 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
      781 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
      841 ccgcagcggg catcacgcat ggtatggatg aactgtacaa aggttcgtaa cttctgactg
      901 agttgcacgc tggtctctcg ctagcctgag acgtactagt agcggccgct gcagggagtt
      961 gtcttcgaag acttcgctct agtcttggac tcctgttgat agatccagta atgacctcag
     1021 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     1081 atccaggggt ccccaataat tacgatttaa atttgtgtct caaaatctct gatgttacat
     1141 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1201 tacaaggggt gttatgagcc atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa
     1261 cagcaacatg gatgcggatc tgtatggcta taaatgggcg cgtgataacg tgggtcagag
     1321 cggcgcgacc atttatcgtc tgtatggcaa accggatgcg ccggaactgt ttctgaaaca
     1381 tggcaaaggc agcgtggcga acgatgtgac cgatgaaatg gtgcgtctga actggctgac
     1441 cgaatttatg ccgctgccga ccattaaaca ttttattcgc accccggatg atgcgtggct
     1501 gctgaccacc gcgattccgg gcaaaaccgc gtttcaggtg ctggaagaat atccggatag
     1561 cggcgaaaac attgtggatg cgctggccgt gtttctgcgt cgtctgcata gcattccggt
     1621 gtgcaactgc ccgtttaaca gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat
     1681 gaacaacggc ctggtggatg cgagcgattt tgatgatgaa cgtaacggct ggccggtgga
     1741 acaggtgtgg aaagaaatgc ataaactgct gccgtttagc ccggatagcg tggtgaccca
     1801 cggcgatttt agcctggata acctgatttt cgatgaaggc aaactgattg gctgcattga
     1861 tgtgggccgt gtgggcattg cggatcgtta tcaggatctg gccattctgt ggaactgcct
     1921 gggcgaattt agcccgagcc tgcaaaaacg tctgtttcag aaatatggca ttgataatcc
     1981 ggatatgaac aaactgcaat ttcatctgat gctggatgaa tttttctaat aattaattgg
     2041 accgcggtcc gcgcgttgtc cttttccgct gcataaccct gcttcggggt cattatagcg
     2101 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     2161 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
     2221 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     2281 aatcctgctc tgcgaggctg gccgtaggcc ggccccgtag aaaagatcaa aggatcttct
     2341 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2401 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2461 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2521 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2581 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2641 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2701 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2761 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2821 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2881 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2941 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3001 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3061 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     3121 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     3181 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     3241 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3301 tgctcccggc atccgcttac agacaagctg tgacagtctc cgggagctgc atgtgtcaga
     3361 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     3421 cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     3481 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     3541 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     3601 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     3661 gttgtgaggg taaacaactg gcggtatgga tgcggcgggg gcgcgcccag ctgtctaggg
     3721 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3781 ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
