LOCUS       sequence-243348-        6826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry cassette for dCas9-KRAB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Genga RMJ, Kernfeld EM, Parsi KM, Parsons TJ, Ziller MJ, Maehr R
  TITLE     Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves 
            Molecular Drivers of Early Human Endoderm Development.
  JOURNAL   Cell Rep. 2019 Apr 16;27(3):708-718.e10. doi: 
            10.1016/j.celrep.2019.03.076.
  PUBMED    30995470
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.03"; journalName: "Cell Rep"; date: 
            "2019-04-16- 16"; volume: "27"; issue: "3"; pages: "708-718.e10"
COMMENT     Sequence Label: pENTR2B-dCas9-KRAB
FEATURES             Location/Qualifiers
     source          1..6826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      257..343
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      435..462
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     470..493
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    512..611
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             662..682
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      731..740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(737..763,764..766,767..3253,3254..3256,3257..4840)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             737..4840
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4844..4870
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4850..4876
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4889..4909
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4916..4936
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5006..5200
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     protein_bind    complement(5209..5308)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5420..5439)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5431..6240
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5498..5517)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(6330..6826,1..92)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     join(6819..6826,1..12)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
       61 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      121 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      181 tggataaccg tattaccgct agcatggatc tcggggacgt ctaactacta agcgagagta
      241 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg gaagactggg cctttcgttt
      301 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
      361 gaacgttgtg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
      421 gcatcaaact aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
      481 tcctgttagt tagttactta agctcgggcc ccaaataatg attttatttt gactgatagt
      541 gacctgttcg ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtacaa
      601 aaaagcaggc tggcgccgga accaattcag tcgacaggcc taggctacgc gcgccaccat
      661 gcccaagaag aagcgcaagg tgggacgcgt ctgcaggata tcaagcttgc ggtaccgcgg
      721 gcccgggatc gccaccatgg acaagaagta cagcatcggc ctggccatcg gcaccaactc
      781 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
      841 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggcgccctgc tgttcgacag
      901 cggagaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
      961 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     1021 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     1081 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     1141 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     1201 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     1261 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     1321 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     1381 actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     1441 tggcctgttc ggcaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     1501 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     1561 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     1621 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     1681 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     1741 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     1801 caagaacggc tacgccggct acatcgatgg cggagccagc caggaagagt tctacaagtt
     1861 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag
     1921 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     1981 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     2041 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     2101 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     2161 ctggaacttc gaggaagtgg tggacaaggg cgccagcgcc cagagcttca tcgagcggat
     2221 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     2281 cgagtacttc accgtgtaca acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     2341 aaagcccgcc ttcctgagcg gcgagcagaa aaaagccatc gtggacctgc tgttcaagac
     2401 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     2461 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     2521 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     2581 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     2641 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     2701 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     2761 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     2821 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     2881 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     2941 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     3001 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     3061 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     3121 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     3181 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     3241 ctacgatgtg gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgataacaa
     3301 agtgctgact cggagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     3361 cgtgaagaag atgaagaact actggcgcca gctgctgaat gccaagctga ttacccagag
     3421 gaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     3481 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     3541 ggactcccgg atgaacacta agtacgacga gaacgacaaa ctgatccggg aagtgaaagt
     3601 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     3661 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     3721 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     3781 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     3841 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     3901 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa acaggcgaga tcgtgtggga
     3961 taagggccgg gactttgcca ccgtgcggaa agtgctgtct atgccccaag tgaatatcgt
     4021 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     4081 cagcgacaag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     4141 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     4201 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     4261 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     4321 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     4381 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     4441 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     4501 acagctgttt gtggaacagc acaaacacta cctggacgag atcatcgagc agatcagcga
     4561 gttctccaag agagtgatcc tggccgacgc taatctggac aaggtgctga gcgcctacaa
     4621 caagcacaga gacaagccta tcagagagca ggccgagaat atcatccacc tgtttaccct
     4681 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     4741 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     4801 gtacgagaca cggatcgacc tgtctcagct gggaggcgac gcctatccct atgacgtgcc
     4861 cgattatgcc agcctgggca gcggctcccc caagaaaaaa cgcaaggtgg aagatcctaa
     4921 gaaaaagcgg aaagtggacg gcattggtag tgggagcaac ggcagcagcg gatccatgga
     4981 cgcgaaatca cttacggcat ggtcgagaac actggttacg ttcaaggacg tgtttgtgga
     5041 ctttacacgt gaggagtgga aattgctgga tactgcgcaa caaattgtgt atcgaaatgt
     5101 catgcttgag aattacaaga acctcgtcag tctcggatac cagttgacga aaccggatgt
     5161 gatccttagg ctcgaaaagg gggaagaacc ttggctggta taatctagac ccagctttct
     5221 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     5281 ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa
     5341 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     5401 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     5461 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     5521 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     5581 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     5641 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     5701 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     5761 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     5821 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     5881 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     5941 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     6001 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     6061 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     6121 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     6181 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     6241 tcagaattgg ttaattggtt gtaacattat tcagattggg ccccgttcca ctgagcgtca
     6301 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6361 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6421 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6481 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6541 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6601 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6661 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6721 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6781 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacg
//
