LOCUS       sequence-243367-        8642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) 
            signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Sattiraju A, Kang S, Giotti B, Chen Z, Marallano VJ, Brusco C, 
            Ramakrishnan A, Shen L, Tsankov AM, Hambardzumyan D, Friedel RH, Zou
            H
  TITLE     Hypoxic niches attract and sequester tumor-associated macrophages 
            and cytotoxic T cells and reprogram them for immunosuppression.
  JOURNAL   Immunity. 2023 Jul 7:S1074-7613(23)00274-1. doi: 
            10.1016/j.immuni.2023.06.017.
  PUBMED    37451265
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2023 Jul 7:S1074-7613(23)00274-1. doi: 
            10.1016/j.immuni.2023.06.017."
COMMENT     Sequence Label: pLenti-HRE-dUnaG
FEATURES             Location/Qualifiers
     source          1..8642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1725..1749
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1998..2036
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2033..2057
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2092..2101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2098..2514
                     /codon_start=1
                     /product="bilirubin-binding green fluorescent protein from 
                     Anguilla japonica (Kumagai et al., 2013)"
                     /label=UnaG
                     /translation="MVEKFVGTWKIADSHNFGEYLKAIGAPKELSDGGDATTPTLYISQ
                     KDGDKMTVKIENGPPTFLDTQVKFKLGEEFDEFPSDRRKGVKSVVNLVGEKLVYVQKWD
                     GKETTYVREIKDGKLVVTLTMGDVVAVRSYRRATE"
     CDS             2683..2712
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2748..2765)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2754..2978
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3020..3044)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3075..3663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3128..3148)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3546..3557)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3677..4176
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3712..3735)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4109..4128
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4197..4796
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4197..4216)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4693..4713
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4934..5167
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5239..5360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5276..5295)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5330..5349
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5400..5535
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5555..5574)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5556..5574)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5584..5601)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5584..5600)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5593..5615)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5742..6197
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5829..5848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6039..6060
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6223..6327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6328..6396,6397..7188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6546..6565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7359..7947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7848..7867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8101..8118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8235..8256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8271..8276,8277..8294,8295..8301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8309..8325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8314..8336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8333..8349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8333..8349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8368..8388
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8370..8388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8416..8642
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
     1741 aagcaggctc cgcggccgcc cccttcaccg taccgagctt ttctcgagcc acagtgcata
     1801 cgtgggctcc aacaggtcct cttgtcgagc cacagtgcat acgtgggctc caacaggtcc
     1861 tcttgtcgag ccacagtgca tacgtgggct ccaacaggtc ctcttgtcga gccacagtgc
     1921 atacgtgggc tccaacaggt cctcttgtcg agccacagtg catacgtggg ctccaacagg
     1981 tcctcttgtc gagatctggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     2041 tagtgaaccg tcagatcact agaagcttgg cattccggta ctgttggtaa agccaccatg
     2101 gtcgagaaat ttgttggcac ctggaagatc gcagacagcc ataattttgg tgaatacctg
     2161 aaagctatcg gagccccaaa ggaattaagc gatggtgggg atgccacgac gccgacattg
     2221 tacatctccc agaaggacgg agacaaaatg acagtgaaaa tagagaatgg acctcctacg
     2281 ttccttgaca ctcaagtaaa gttcaaatta ggggaggagt tcgacgaatt tccttctgat
     2341 cgaagaaaag gcgtaaaatc tgtcgtgaac ttggtgggag agaagctggt gtacgtacaa
     2401 aagtgggacg gcaaggagac gacgtatgtc cgagagataa aggacggtaa actggtcgtg
     2461 acacttacga tgggagacgt cgtggctgtg cgcagctacc ggagggcgac ggaaaccggt
     2521 aagaagctta gccacggctt cccgccggag gtggaggagc aggatgatgg cacgctgccc
     2581 atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc ttctgctagg
     2641 atcaatgtgg tcgacccgcg gggatccacg cgtgcggccg cagaacaaaa actcatctca
     2701 gaagaggatc tgaatggggc cgcatagtct agaagctcgc tgatcagcct cgactgtgcc
     2761 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2821 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2881 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2941 caatagcagg catgctgggg atggcccggg ctctatggct tctgaggcgg aaagaaccag
     3001 ctgaagggtg ggcgcgccga cccagctttc ttgtacaaag tggttgatat ccagcacagt
     3061 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3121 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3181 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3241 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3301 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3361 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3421 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     3481 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3541 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3601 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3661 cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     3721 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     3781 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     3841 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     3901 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     3961 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     4021 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     4081 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     4141 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     4201 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     4261 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     4321 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     4381 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     4441 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     4501 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     4561 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     4621 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     4681 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     4741 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     4801 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc
     4861 ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4921 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
     4981 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5041 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5101 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5161 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5221 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5281 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5341 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     5401 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5461 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5521 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5581 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5641 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5701 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     5761 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5821 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5881 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5941 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6001 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6061 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6121 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6181 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6241 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6301 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6361 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6421 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6481 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6541 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6601 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6661 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6721 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6781 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6841 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6901 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6961 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7021 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7081 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7141 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7201 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7261 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7321 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7381 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7441 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7501 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7561 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7621 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7681 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7741 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7801 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7861 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7921 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7981 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8041 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8101 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8161 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     8221 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8281 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8341 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     8401 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     8461 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     8521 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     8581 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     8641 ac
//
