LOCUS       sequence-243368-       10579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aka-Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Bozec D, Sattiraju A, Bouras A, Jesu Raj JG, Rivera D, Huang Y, 
            Junqueira Alves C, Tejero R, Tsankova NM, Zou H, Hadjipanayis C, 
            Friedel RH
  TITLE     Akaluc bioluminescence offers superior sensitivity to track in vivo 
            glioma expansion.
  JOURNAL   Neurooncol Adv. 2020 Oct 10;2(1):vdaa134. doi: 
            10.1093/noajnl/vdaa134. eCollection 2020 Jan-Dec.
  PUBMED    33241215
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurooncol 
            Adv."; date: "2020-10-10"; volume: "2(1):vdaa134. doi"; pages: " 
            10.1093/noajnl/vdaa134. eCollection 2020 Jan-Dec"
COMMENT     Sequence Label: pLenti-PGK-Venus-Akaluc (neo)
FEATURES             Location/Qualifiers
     source          1..10579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(138..140,141..143,144..854)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(183..204)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(444..463)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     791..812
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2530..2554)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2585..3173
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2638..2658)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3056..3067)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3216..3715
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3251..3274)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3648..3667
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3729..4532
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3792..3811)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4402..4421
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4623..4856
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4928..5049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4965..4984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5019..5038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5089..5224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5244..5263)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5245..5263)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5273..5290)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5273..5289)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5282..5304)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5431..5886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5518..5537)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5728..5749
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5912..6016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6017..6085,6086..6877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6235..6254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7048..7636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7537..7556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7790..7807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7924..7945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7960..7965,7966..7983,7984..7990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7998..8014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8003..8025
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8022..8038
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8022..8038
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8057..8077
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8059..8077
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8105..8331
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8332..8512
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8559..8684
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9177..9410
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9595..9639
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9906..10023
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10069..10579
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10498..10516
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ggggatccac cggttcgtcg actagtccag tgtggtggaa ttctgcagat atcaacaagt
       61 ttgtacaaaa aagcaggctc cgcggccgcc cccttcaccg acatagtaca gaagggtggg
      121 cgcgcccacc cttcaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      181 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      241 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
      301 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct
      361 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      421 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      481 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      541 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg
      601 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
      661 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      721 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      781 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      841 acgagctgta caagggatcc atggaagatg ccaaaaacat taagaagggc ccagcgccgt
      901 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
      961 ccctggtgcc cggcgccatc gcctttaccg acgcacatat tcaggtggac gttacctacg
     1021 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaggcgctat gggctgaata
     1081 caaaccatcg gatcgtggtg tgcagcgaga atagctcgca gttcttcatg cccgtgttgg
     1141 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
     1201 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
     1261 gaaaggtcct caacgtgcaa aagaagctac cgatcatacg aaagatcatc atcatggata
     1321 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
     1381 ccagcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
     1441 tgatcatgaa cagtagtggt agtacaggat tacccaaggg cgtagcccta ccgcaccgca
     1501 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggctaccag aacatccccg
     1561 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
     1621 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
     1681 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
     1741 gcctcgctaa gagcactctc atcgacaagt acgacctaag cagcttgcgc gagatcgcca
     1801 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccgcctac
     1861 caggcatccg ccagggctat ggcctgacag aaacaaccaa cgccgtcatg atcacccccg
     1921 agggggaccg taagcctggc tcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
     1981 tagacttggt caccggtaag acactgggtg tgaaccagcg cggtgagctg tgcgtccgtg
     2041 gccccatgat catgagcggc tacgttaaca accccgaggc tacgaacgct ctcatcgaca
     2101 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
     2161 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
     2221 tggagggcat cctgctgcaa cacccctaca tcttcgacgc cggagtcgcc ggcctgcccg
     2281 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgtt ggaacacggt aaaaccatga
     2341 ccgagaaaga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
     2401 gtggtgttgt gtttgtggat gaagtcccta gaggatcgac cggcaagtta gacgcccgca
     2461 agatccgcga gattctcact aaggccaaga aggacggcaa gatcgccgtg taaaagggtg
     2521 ggcgcgccga cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     2581 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2641 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2701 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2761 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2821 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2881 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2941 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     3001 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3061 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3121 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     3181 tctgcagata tccggactag tgatctaatt ctaccgggta ggggaggcgc ttttcccaag
     3241 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     3301 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     3361 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     3421 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     3481 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     3541 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     3601 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     3661 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     3721 cagccaatat gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt
     3781 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3841 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3901 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3961 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4021 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4081 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4141 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4201 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4261 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4321 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4381 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4441 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4501 ccttctatcg ccttcttgac gagttcttct gaggggatcc gtcgagggcc cggtaccttt
     4561 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4621 actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4681 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4741 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4801 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     4861 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     4921 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5041 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     5101 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5161 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5221 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     5281 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     5341 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     5401 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     5461 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5521 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5581 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5641 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5701 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5761 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5821 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     5881 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5941 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6001 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6061 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6121 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6181 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6241 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6301 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6361 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6421 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6481 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6541 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6601 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6661 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6721 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6781 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6841 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6901 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6961 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7021 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7081 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7141 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7201 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7261 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7321 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7381 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7441 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7501 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7561 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7621 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7681 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7741 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7801 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7861 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7921 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7981 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     8041 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     8101 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     8161 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     8221 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     8281 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     8341 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8401 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8461 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     8521 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     8581 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     8641 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8701 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     8761 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     8821 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     8881 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     8941 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     9001 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     9061 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     9121 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     9181 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     9241 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     9301 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     9361 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     9421 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     9481 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     9541 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     9601 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     9661 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     9721 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     9781 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     9841 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     9901 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     9961 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
    10021 tttatcgatc acgagactag cctcgagaag cttgatatcg aattccacgg ggttggggtt
    10081 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
    10141 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
    10201 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
    10261 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
    10321 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
    10381 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
    10441 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
    10501 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
    10561 caccgacctc tctccccag
//
