LOCUS       sequence-243382-       12767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains 
            dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 
            bp upstream of HSP90 start codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12767)
  AUTHORS   Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS
  TITLE     A CRISPR Interference Platform for Efficient Genetic Repression in 
            Candida albicans.
  JOURNAL   mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 
            10.1128/mSphere.00002-19.
  PUBMED    30760609
REFERENCE   2  (bases 1 to 12767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2019-02-13"; volume: "4(1). pii: 4/1/e00002-19. doi"; pages: 
            " 10.1128/mSphere.00002-19"
COMMENT     Sequence Label: pRS267
FEATURES             Location/Qualifiers
     source          1..12767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     207..224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    341..362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(377..382,383..400,401..407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    415..431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     420..442
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     439..455
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     439..455
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        476..494
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             join(1528..1554,1555..1557,1558..4113,4114..4116,
                     4117..5631)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5644..5664
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(6958..7155)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(7756..8099)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(9062..9081)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9063..9081)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9088..9105)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9088..9104)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9097..9119)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9333..9352)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     misc_RNA        complement(9389..9463)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     10912..10933
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11096..11200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11201..11269,11270..12061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11419..11438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12232..12767,1..53)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12721..12740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       61 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg taagagcgcc
      241 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      301 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      361 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      421 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta
      481 accctcacta aagggaacaa aagctggtac cagagctatt aagatcacca gcctcgttta
      541 taataaactt agtctaatat ttcttttgga ccaaaataga aactgttctc aggtggcaaa
      601 aaacagctta aagtctgtgt taaaaaaaga tctcggctat gccaatcaaa aaggagtgac
      661 attattatgt aaaagaagaa atgttgtaat tccgcgaaac aacaatttta atgcttactg
      721 tgccgccacc aatatcctat ggccaagggg cctttaaaca tgccacggaa atatacagtc
      781 aaagtaaacc tactatgtct ttagagcctt caggatcaat tgtttttaaa tgaatgactg
      841 caaaactaga aaagtagcct cttacataag caagtcattt gtacaccgag agtttagtaa
      901 ccagtgaagc aagtatatct ggcaaacctg aaacttatct gtgaattata aagctcataa
      961 gtaaaaagtg tagaaaagaa gataaggcag accaaataca aatttgcaac agtacaacga
     1021 atatttcagc gtcaaaacta gagaataata aagaaaacga tcttttcaaa aagaaaaaac
     1081 cttttagttt tcctttgttg ttgttgtggg tgtgtgctat ttatattata tagtttactc
     1141 ataataccat aaaatattcg gtttgattag gttattttaa taagctaatt tgtttctaat
     1201 cgtgtaattt atgctgtgta tattaagtag tgtgtgcact gcccaaaaat gtttgttgtt
     1261 tatagtcggt taaagagaaa aaagaaaaaa agatccatac acacacgtta attagttgtt
     1321 caacgtaata cactcatatt ttgttcttat ttgctttcgg tcgctgttct caccaagatt
     1381 tattgccaac gaaacaattt ttttttatat attttcagat ttttcttttt ttcctttcct
     1441 ttccttttct aattttcact cctggttttc tttctttctt agaaacatta tctcgatatt
     1501 aatattaaaa aaatataatc attcaaaatg gataaaaaat actccatagg tttggccatt
     1561 ggaaccaatt cagttggatg ggctgtaatc actgatgaat ataaggttcc ttctaaaaag
     1621 ttcaaagttt taggaaatac tgatagacac tccattaaaa agaatttaat tggtgccttg
     1681 ttgtttgaca gtggtgaaac agctgaagca actcgtttga aaagaacagc tagaagaaga
     1741 tataccagaa gaaagaacag aatttgttac ttgcaagaaa tcttttctaa tgaaatggca
     1801 aaggttgatg attctttttt tcatagattg gaagaaagtt tcttagttga agaagataag
     1861 aaacatgaaa gacatccaat ctttggtaat attgtagatg aagtggctta ccacgaaaaa
     1921 tatcctacaa tttatcattt gagaaaaaaa ttagttgact ccactgataa agctgactta
     1981 agacttatat atttggcatt ggctcacatg atcaagttca gaggtcactt cttaattgaa
     2041 ggtgatttga atccagataa ttccgatgtc gacaagttgt ttattcaatt ggttcaaact
     2101 tacaaccaac ttttcgagga aaatccaatc aatgcctcag gtgtggatgc taaagctatt
     2161 ttatctgcta gattgtcaaa aagtagacgt ttggaaaatc ttattgccca attgccaggt
     2221 gaaaaaaaaa atggattgtt tggaaactta attgcattat ctttgggttt gaccccaaat
     2281 ttcaaatcaa actttgattt ggccgaggat gctaaacttc aattgtctaa ggatacatac
     2341 gatgacgatt tagataatct tcttgcccaa attggtgatc aatatgcaga tttattcttg
     2401 gcagctaaga acttgagtga tgcaattttg ttaagtgaca ttttgagagt caacacagaa
     2461 attacaaaag ctcctttatc cgcctctatg attaaaagat atgatgaaca ccatcaagat
     2521 ttaaccttgt tgaaagctct tgtacgtcaa caattaccag aaaagtataa agaaatattc
     2581 tttgatcaat ctaaaaatgg ttacgctggt tatattgatg gtggtgctag tcaagaagaa
     2641 ttttataagt ttatcaagcc aattcttgaa aaaatggatg gtactgagga attgttggtt
     2701 aagttgaatc gagaggattt acttcgtaag caaagaacat ttgataacgg ttctatccct
     2761 catcaaatcc acttaggtga gttacacgct atcttgagac gtcaggaaga tttttaccct
     2821 tttttgaaag acaatcgtga aaaaattgaa aaaattttga ctttcagaat tccttattac
     2881 gtcgggccat tggctagagg taattctaga tttgcatgga tgactcgtaa atctgaagag
     2941 actatcaccc catggaattt tgaggaagtt gttgataagg gagcctccgc tcaatcattc
     3001 atcgagagaa tgaccaattt cgacaaaaac ttgccaaatg agaaggtgtt acctaaacat
     3061 tctttgttat atgaatattt cactgtttat aatgaattaa caaaagtcaa atacgtcact
     3121 gaaggtatga gaaaaccagc attcttaagt ggtgaacaaa agaaagctat cgttgacttg
     3181 ttattcaaaa ctaatcgtaa agtcactgtc aaacaactta aagaggacta ttttaagaaa
     3241 atagagtgtt tcgattccgt tgaaatcagt ggggttgagg atagatttaa tgctagttta
     3301 ggaacttatc acgatttgtt gaaaattatc aaagacaaag attttttgga taatgaagaa
     3361 aacgaagata tcttggagga cattgtattg actttgactt tgtttgagga tagagaaatg
     3421 atcgaagaac gtttaaaaac ttacgctcac ttattcgacg ataaagtcat gaaacaatta
     3481 aaaagaagaa gatataccgg ttggggaaga ttatctagaa agttgattaa tggtattaga
     3541 gataagcaaa gtggtaaaac catcttagat ttccttaaaa gtgatggttt tgcaaacaga
     3601 aactttatgc agttgattca tgatgatagt ttgaccttta aggaagatat tcaaaaagct
     3661 caggtttctg gacagggtga ttcattgcat gaacatattg ccaacttagc agggtctcca
     3721 gctatcaaaa agggaatttt acaaactgtt aaagttgttg atgagttggt gaaagttatg
     3781 ggacgtcata agcctgaaaa tatagttata gaaatggcta gagaaaatca aacaactcag
     3841 aaaggacaaa agaattctcg tgaaagaatg aaaagaatag aagaaggaat taaagagttg
     3901 ggttctcaaa tcttaaaaga acacccagtc gaaaacaccc agttacagaa cgagaagctt
     3961 tacttgtact atttgcaaaa cgggcgtgac atgtatgtag atcaagagtt ggatatcaat
     4021 agactttcag attacgatgt cgatcacata gttcctcagt catttttaaa agatgattcc
     4081 attgacaaca aagttttaac aagatctgat aaggctagag gtaaatcaga caatgtgcca
     4141 tcagaagaag tggttaagaa aatgaagaac tattggagac aattgttaaa tgcaaagttg
     4201 attactcaga gaaaatttga caacttaact aaagctgaaa gaggaggatt atccgaatta
     4261 gataaagctg gattcataaa acgtcagttg gttgaaacaa gacaaattac taaacatgta
     4321 gcccaaattt tagattcccg aatgaatact aaatatgatg aaaatgacaa attaatcaga
     4381 gaggtcaaag tgatcacatt aaagtcaaag ttggtgtcag atttccgaaa ggattttcaa
     4441 ttctacaagg tgagagagat caacaattat catcatgctc acgacgcata tttgaatgca
     4501 gtggtaggaa ctgccttaat aaagaagtat cctaagttag aatccgaatt cgtgtatggt
     4561 gattataaag tctacgatgt cagaaagatg atagcaaaat cagaacaaga aattgggaaa
     4621 gccaccgcaa agtatttctt ttactccaac atcatgaatt tctttaagac cgaaatcacc
     4681 ttggctaacg gggaaatcag aaaacgtcct ttgattgaaa caaatggaga aaccggtgaa
     4741 attgtgtggg ataaggggag agattttgct actgttagaa aagttttatc aatgcctcaa
     4801 gttaatattg taaagaagac tgaagttcaa actggtggtt tctctaagga atccattctt
     4861 ccaaaacgaa actcagacaa gttgatcgcc cgaaaaaaag attgggatcc taagaaatac
     4921 ggaggttttg attctcctac tgtcgcatat tctgtcttgg tcgtggctaa agttgaaaag
     4981 ggtaaatcca aaaaattgaa atctgtgaaa gaattgttag gtattaccat catggagcgt
     5041 tcatcctttg agaaaaaccc aattgacttt ctcgaagcga agggctataa agaagtgaag
     5101 aaagatttga ttattaagtt gccaaagtat tctctctttg aacttgaaaa tgggagaaag
     5161 agaatgttgg cttcagctgg agaattgcaa aaaggaaacg agttagcttt gccttcaaag
     5221 tacgttaatt tcttatactt agcatctcat tatgaaaagt tgaaggggtc ccctgaagac
     5281 aatgagcaaa agcaattatt cgtagaacaa cataaacact atttggatga gataattgaa
     5341 caaatctccg agttttctaa aagagttatt ttggctgatg caaatttgga taaagtttta
     5401 tctgcttata ataagcatcg tgacaaacca attcgagaac aagctgaaaa tattatccac
     5461 ttgttcacat tgactaactt gggagctcca gccgcgttta agtactttga caccaccatt
     5521 gaccgtaaga gatatacttc aacaaaagaa gtgttagacg ccacattgat tcatcagtcc
     5581 ataactggtt tatatgaaac tagaatcgat ttgtcacaat tggggggtga tagccgagcg
     5641 gatcctaaga aaaaacgaaa agtcagtggt ggtggtagtg ggtccgggtc aggaggttca
     5701 tccaagttag gaggttcagg agggagtatg gaacgagtta gaatgatcaa cgtacagcgt
     5761 ttgttagagg ctgcagagtt tttggaaaga cgagaacgag aatgcgagca tggttacgca
     5821 tcttccttcc catctatgcc atccccacga gggagtggag gggggtctgg gtccggttct
     5881 ggatctggga gttcaaaatt agggggatcc gggggatcta tggaacgtgt ccgtatgatt
     5941 aatgtccaaa gattattaga agccgcagaa tttttagaaa gacgagaaag agaatgtgaa
     6001 catggttatg catcatcatt tccaagtatg ccttcaccaa gaggttaacc cgggggatcc
     6061 gagtgaaatt ctggaaatct ggaaatctgg ttttgtattc ttgttattct tctttttgtt
     6121 attacatata taacttgtta cttttttaaa aaaatctttg tttattttat aaatatataa
     6181 aactaaattt aagaagaaga gaaaaatgtt ttatttgaga gattgaaatt ttacttgaat
     6241 ttagcttagc ttttataaag tattattatg taaaaaaaca aaacaaatat acattaaaaa
     6301 gttaagacta taaaatagcc acccaaggca tttctatatc ttgttgttgt tgttttcatc
     6361 ttctgtatca gaggaactta ttttattatt ttcgtcacgg gtattttctc ttgtttgatg
     6421 attcatccca ttcattccat cataaaattc tagaaggacc acctttgatt gtaaatagta
     6481 ataattacca cccttattta attatttatt taacttattt atttatttat tatacatata
     6541 tacaaatcta ataaagtgaa aatctccccc ttcacattta taattggcca gtttttttca
     6601 aataagcatt ccaaccagca tctctatacc ttttaccttc aatatctgga tctcttcctt
     6661 taccaaacaa tcctctacca acaatgataa tatcagttcc agtgctaaca acttcatcaa
     6721 cagttctata ttgttgtcct aatccatcac ctttatcatc taatccaact ccaggtgtca
     6781 taataagcca atcaaatcct tcttcttgtc cacccatatc acgttgggca ataaatccaa
     6841 taacaaattc cttatcggat ttagcaattt caacagtttt ttgagaatat tctccatacg
     6901 aattcgagct cgtttaaacg tcgacactgg atggcggcgt tagtatcgaa tcgacagcag
     6961 tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg cacttaactt
     7021 cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa catttgatta
     7081 aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga aaacaagaat
     7141 ctttttattg tcagtactga ctcgagttat tatggacatg gcatagacat atacaaagct
     7201 tgttcaccat cggaagcagt accatcgtat aaagcagtat ccaaaccaca caaagtgaaa
     7261 cccattcttc tataagcatg aatagctgga gcattaacat tggtaacttc caaccacaaa
     7321 tgaccagcac ctctttctct ggcgaattca gtagccaaac ccatcaaagc tctaccaaca
     7381 ccatgacctc tatgttctgg agcaacttca atatcttcaa cagtcaatct tctgttccaa
     7441 ccagaataag aaacaacaac gaaaccagcc aaatcaccat catcaccata agcaacgaaa
     7501 gttctagaat ctggatcacc atcttcacca gcatcggatt catcatcgga ttcatcatct
     7561 gggaaaacct tagtcaatgg tggatcaact ggaacttctc tcaaagtgaa accatcacca
     7621 gtagcagtaa ctctaaaaac agtatcggta gtgaaagaac catccaaagc ttcaatagct
     7681 tcagcatcac ctggaacaga agttctgtat ctataagcag tatcatccaa agtagtagac
     7741 ataattgtag gatccggttg tttatgttcg gatgtgatgt gagaactgta tcctagcaag
     7801 attttaaaag gaagtatatg aaagaagaac ctcagtggca aatcctaacc ttttatattt
     7861 ctctacaggg gcgcggcgtg gggacaattc aacgcgtctg tgaggggagc gtttccctgc
     7921 tcgcaggtct gcagcgagga gccgtaattt ttgcttcgcg ccgtgcggcc atcaaaatgt
     7981 atggatgcaa atgattatac atggggatgt atgggctaaa tgtacgggcg acagtcacat
     8041 catgcccctg agctgcgcac gtcaagactg tcaaggaggg tattctgggc ctccatgtcg
     8101 ctggccgggt gacccggcgg ggacgaggca agcttgatat gctaatgatc ccactgatga
     8161 taattcagct aacatcaata accctcttgg ctcttggttg gtggtggttt ctttagctcc
     8221 ctgttttaat ccttcaacta ctccattccc agtgacacca tgagcattag taatatctgc
     8281 ccaactacta attttataaa ctccaccaat atattgtttc ttcacggtat taccaatatc
     8341 agcaaatttt ctatcttcaa aaatcataaa ttgatgttta cgtgaaagtt ctaataatgg
     8401 ttcaatagtg gattcatagg aaaaatcatt gattatatca atatgagtct tgattaagca
     8461 tacataagga cccaatttat caattaattc aaggaattcc ttagtggtat caacatcaat
     8521 tgatgcacat aaattggttt tcttcagttc cattaatcga aataatcgtt gtgctactgg
     8581 tgaggcatga gtttctgctc tctcactata ggtcttagtg ttgactgtca tatcttttgt
     8641 ttgctatttc tatttctatt tctttcttca actgtaaaag gggcgatata taaaccagtg
     8701 tgtatggggt tgttgctcag gaattgatca atgtttaaaa gaatggttga aatcatcatt
     8761 gaagaacaaa aaaaaagatt ttttctttca ttatgttatc tgtataatat gcaggaaata
     8821 agattgcaaa aaaatagccg tttttttgga tactatcaaa caagaggaat gactaataca
     8881 gaattagtgt tacgaatcaa tggcactaca gcaactttca acaaaataat ctacgttcaa
     8941 aatataatat atactattaa ataaaatcaa ataataaaat aattttaaag aaagtagtac
     9001 cagctctttt ttttgtttcc gtttatacca tccaaatcaa ttcctattag tctccaattc
     9061 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     9121 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     9181 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     9241 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9301 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9361 cgccacgttc gccggcaaaa aaaagcacca ccgactcggt gccacttttt caagttgata
     9421 acggactagc cttattttaa cttgctattt ctagctctaa aacgtggtct ccgttggttg
     9481 ccatcgaaat taaaaatagt ttacgcaagt ctcgaacttc caccacaatt taattgtaag
     9541 aacgctgcga aaacatattc tccaatccta ttgggccgaa gtttcagcta tgccaaaggc
     9601 atcaaatttt tttttataaa attttccact tttccatacc agtgaaatta tcacgtgatg
     9661 gagtgagtat ctataatatt tgattgtttc tttgatgagg caatactcga gtatagttat
     9721 tgaccaatga cttttaccgg ctaaacaagt ggtattcaag cacaattcag ttttctggat
     9781 ctctaataat tgcaattgca attgcttcac atactctgtt tataattcag tttctttctc
     9841 ttgatgttac tgatatatta agttaataaa taccaataac tttttttcaa aaatcttcta
     9901 ttttcctttc aattgattat ttaaacacga atcgttaata agctgtgatt gctttttctc
     9961 tttctttttc ttttctttgt tcaagtttga aaaaaaaaaa aaaaggtttc tcttgttcga
    10021 tgtagaattc agtgggttat agttgtaatg atatatggaa taaaaagaaa gaaagagaag
    10081 agaaagagaa gaagagaaga attaagtaaa cgtgtattag aatttaaaaa ttaaaattca
    10141 tgataatctt taataataat taactaagaa tcacaaaatt gaaaatttga aagaaaaaag
    10201 atcactccaa ggtcgtgtca agtgactaga aattcttaat taaaatagtt ataacaaaaa
    10261 aagaaatttg tatataacaa ttaagtaaaa gaaaaaaaag caattcgctt tgtataataa
    10321 tacccagaaa aatatattca tatattcata aatcaaacat gaaatgaaga atgctgaatc
    10381 acttgatagg atttagttcc attatggtac taagaagtaa tattacttct atgaaaaggt
    10441 tgaatccgag atcaaaattc atcttttcat atactctgga gaaaaaaaaa aaagtaattg
    10501 tagtaagaat gacaagtatc agttgagaaa aaaagtaaca aagagaaagc tcggaggagg
    10561 ctccccaaag gttttatcac caatggtggt accactaacc caagaacaga aaaagcattg
    10621 atattttgaa aaatacaata ctcatctttt aacccaaacc ttttcttgtc ctttaaaact
    10681 tttatctcat gtctgtccaa agtcatattg tcttactact ttctctcctt caacgtcctt
    10741 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    10801 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    10861 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    10921 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    10981 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
    11041 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
    11101 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    11161 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    11221 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    11281 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    11341 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    11401 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    11461 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    11521 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    11581 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    11641 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    11701 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    11761 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    11821 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    11881 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    11941 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    12001 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    12061 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    12121 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    12181 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    12241 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    12301 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    12361 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    12421 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    12481 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    12541 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    12601 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    12661 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    12721 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctct
//
