LOCUS       sequence-243383-        9745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Kawaguchi Y, Kovacs JJ, McLaurin A, Vance JM, Ito A, Yao TP
  TITLE     The deacetylase HDAC6 regulates aggresome formation and cell 
            viability in response to misfolded protein stress.
  JOURNAL   Cell. 2003 Dec 12. 115(6):727-38.
  PUBMED    14675537
REFERENCE   2  (bases 1 to 9745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2003 
            Dec 12. 115(6):727-38."
COMMENT     Sequence Label: pcDNA-HDAC6.DC-FLAG
FEATURES             Location/Qualifiers
     source          1..9745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        174..553
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        554..757
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     708..728
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     802..821
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        802..820
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          857..1429
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             5195..5218
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5237..5255)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5238..5255)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5275..5292)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5281..5505
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5551..5979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5638..5657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5848..5869
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5988..6008)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5993..6322
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6173..6308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6235..6254
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6389..7183
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6443..6462)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7053..7072
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7357..7478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7394..7413)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7448..7467
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7527..7543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7527..7543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7540..7562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7551..7567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7575..7581,7582..7599,7600..7605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7620..7641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7758..7775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7929..8517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8009..8028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8688..9479,9480..9548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9311..9330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9549..9653)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(9728..9745,1..2))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
       61 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      121 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      181 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      241 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      301 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      361 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      421 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      481 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      541 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      601 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      661 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      721 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      781 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctagagtcga
      841 tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattgt
      901 aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg
      961 tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc tactctgttg
     1021 acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt taaactttag
     1081 cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt gtaagtactt
     1141 tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt cagcacagtt
     1201 ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg
     1261 ctggcgtgga aatattctta ttggtagaaa caactacatc ctggtcatca tcctgccttt
     1321 ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa
     1381 accgggcccc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     1441 cgtgctggtt tgtgctgtcg accccaagct tggcccgggc ggatccacta gtaacggccg
     1501 ccagtgtgct ggaattctgc agatatccat cacactggcg gccgccatga cctcaaccgg
     1561 ccaggattcc accacaacca ggcagcgaag aagtaggcag aacccccagt cgccccctca
     1621 ggactccagt gtcacttcga agcgaaatat taaaaaggga gccgttcccc gctctatccc
     1681 caatctagcg gaggtaaaga agaaaggcaa aatgaagaag ctcggccaag caatggaaga
     1741 agacctaatc gtgggactgc aagggatgga tctgaacctt gaggctgaag cactggctgg
     1801 cactggcttg gtgttggatg agcagttaaa tgaattccat tgcctctggg atgacagctt
     1861 cccggaaggc cctgagcggc tccatgccat caaggagcaa ctgatccagg agggcctcct
     1921 agatcgctgc gtgtcctttc aggcccggtt tgctgaaaag gaagagctga tgttggttca
     1981 cagcctagaa tatattgatc tgatggaaac aacccagtac atgaatgagg gagaactccg
     2041 tgtcctagca gacacctacg actcagttta tctgcatccg aactcatact cctgtgcctg
     2101 cctggcctca ggctctgtcc tcaggctggt ggatgcggtc ctgggggctg agatccggaa
     2161 tggcatggcc atcattaggc ctcctggaca tgccgcccag cacagtctta tggatggcta
     2221 ttgcatgttc aaccacgtgg ctgtggcagc ccgctatgct caacagaaac accgcatccg
     2281 gagggtcctt atcgtagatt gggatgtgca ccacggtcaa ggaacacagt tcaccttcga
     2341 ccaggacccc agtgtcctct atttctccat ccaccgctac gagcagggta ggttctggcc
     2401 ccacctgaag gcctctaact ggtccaccac aggtttcggc caaggccaag gatataccat
     2461 caatgtgcct tggaaccagg tggggatgcg ggatgctgac tacattgctg ctttcctgca
     2521 cgtcctgctg ccagtcgccc tcgagttcca gcctcagctg gtcctggtgg ctgctggatt
     2581 tgatgccctg caaggggacc ccaagggtga gatggccgcc actccggcag ggttcgccca
     2641 gctaacccac ctgctcatgg gtctggcagg aggcaagctg atcctgtctc tggagggtgg
     2701 ctacaacctc cgcgccctgg ctgaaggcgt cagtgcttcg ctccacaccc ttctgggaga
     2761 cccttgcccc atgctggagt cacctggtgc cccctgccgg agtgcccagg cttcagtttc
     2821 ctgtgctctg gaagcccttg agcccttctg ggaggttctt gtgagatcaa ctgagaccgt
     2881 ggagagggac aacatggagg aggacaatgt agaggagagc gaggaggaag gaccctggga
     2941 gccccctgtg ctcccaatcc tgacatggcc agtgctacag tctcgcacag ggctggtcta
     3001 tgaccaaaat atgatgaatc actgcaactt gtgggacagc caccaccctg aggtacccca
     3061 gcgcatcttg cggatcatgt gccgtctgga ggagctgggc cttgccgggc gctgcctcac
     3121 cctgacaccg cgccctgcca cagaggctga gctgctcacc tgtcacagtg ctgagtacgt
     3181 gggtcatctc cgggccacag agaaaatgaa aacccgggag ctgcaccgtg agagttccaa
     3241 ctttgactcc atctatatct gccccagtac cttcgcctgt gcacagcttg ccactggcgc
     3301 tgcctgccgc ctggtggagg ctgtgctctc aggagaggtt ctgaatggtg ctgctgtggt
     3361 gcgaccccca ggacacgccg cagagcagga tgcagcttgc ggtttttgct ttttcaactc
     3421 tgtggctgtg gctgctcgcc atgcccagac tatcagtggg catgccctac ggatcctgat
     3481 tgtggattgg gatgtccacc acggtaatgg aactcagcac atgtttgagg atgaccccag
     3541 tgtgctatat gtgtccctgc accgctatga tcatggcacc ttcttcccca tgggggatga
     3601 gggtgccagc agccagatcg gccgggctgc gggcacaggc ttcaccgtca acgtggcatg
     3661 gaacgggccc cgcatgggtg atgctgacta cctagctgcc tggcatcgcc tggtgcttcc
     3721 cattgcctac gagtttaacc cagaactggt gctggtctca gctggctttg atgctgcacg
     3781 gggggatccg ctggggggct gccaggtgtc acctgagggt tatgcccacc tcacccacct
     3841 gctgatgggc cttgccagtg gccgcattat ccttatccta gagggtggct ataacctgac
     3901 atccatctca gagtccatgg ctgcctgcac tcgctccctc cttggagacc caccacccct
     3961 gctgaccctg ccacggcccc cactatcagg ggccctggcc tcaatcactg agaccatcca
     4021 agtccatcgc agatactggc gcagcttacg ggtcatgaag gtagaagaca gagaaggacc
     4081 ctccagttct aagttggtca ccaagaaggc accccaacca gccaaaccta ggttagctga
     4141 gcggatgacc acacgagaaa agaaggttct ggaagcaggc atggggaaag tcacctcggc
     4201 atcatttggg gaagagtcca ctccaggcca gactaactca gagacagctg tggtggccct
     4261 cactcaggac cagccctcag aggcagccac agggggagcc actctggccc agaccatttc
     4321 tgaggcagcc attgggggag ccatgctggg ccagaccacc tcagaggagg ctgtcggggg
     4381 agccactccg gaccagacca cctcagagga gactgtggga ggagccattc tggaccagac
     4441 cacctcagag gatgctgttg ggggagccac gctgggccag actacctcag aggaggctgt
     4501 aggaggagct acactggccc agaccatctc ggaggcagcc atggagggag ccacactgga
     4561 ccagactacg tcagaggagg ctccaggggg caccgagctg atccaaactc ctctagcctc
     4621 gagcacagac caccagaccc ccccaacctc acctgtgcag ggaactacac cccagatatc
     4681 tcccagtaca ctgattggga gtctcaggac cttggagcta ggcagcgaat ctcagggggc
     4741 ctcagaatct caggccccag gagaggagaa cctactagga gaggcagctg gaggtcagga
     4801 catggctgat tcgatgctga tgcagggatc taggggcctc actgatcagg ccatatttta
     4861 tgctgtgaca ccactgccct ggtgtcccca tttggtggca gtatgcccca tacctgcagc
     4921 aggcctagac gtgacccaac cttgtgggga ctgtggaaca atccaagaga attgggtgtg
     4981 tctctcttgc tatcaggtct actgtggtcg ttacatcaat ggccacatgc tccaacacca
     5041 tggaaattct ggacacccgc tggtcctcag ctacatcgac ctgtcagcct ggtgttacta
     5101 ctgtcaggcc tatgtccacc accaggctct cctagatgtg aagaacatcg cccaccagaa
     5161 caagtttggg gaggatatgc cccacccaca cggggattat aaagatgatg atgataaata
     5221 aactagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     5281 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5341 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5401 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5461 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5521 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5581 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5641 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5701 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5761 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5821 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5881 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5941 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6001 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6061 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6121 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6181 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6241 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6301 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6361 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     6421 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6481 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6541 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6601 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6661 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6721 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6781 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6841 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6901 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6961 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7021 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7081 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     7141 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     7201 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     7261 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7321 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7381 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7441 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7501 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7561 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7621 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7681 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7741 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7801 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7861 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7921 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7981 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8041 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8101 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8161 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8221 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8281 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8341 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8401 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8461 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8521 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8581 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8641 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8701 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8761 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8821 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8881 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8941 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9001 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9061 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9121 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9181 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9241 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9301 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9361 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9421 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9481 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9541 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9601 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9661 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     9721 ctctcagtac aatctgctct gatgc
//
