LOCUS       sequence-243395-        8574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luminescent reporter of insulin secretion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Burns SM, Vetere A, Walpita D, Dancik V, Khodier C, Perez J, Clemons
            PA, Wagner BK, Altshuler D
  TITLE     High-throughput luminescent reporter of insulin secretion for 
            discovering regulators of pancreatic Beta-cell function.
  JOURNAL   Cell Metab. 2015 Jan 6;21(1):126-37. doi: 
            10.1016/j.cmet.2014.12.010.
  PUBMED    25565210
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.12"; journalName: "Cell Metab"; date: 
            "2015-01-6- 6"; volume: "21"; issue: "1"; pages: "126-37"
COMMENT     Sequence Label: Proinsulin-NanoLuc in pLX304
FEATURES             Location/Qualifiers
     source          1..8574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..64
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    181..202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(217..222,223..240,241..247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    255..271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     260..282
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     279..295
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     279..295
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     314..334
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        316..334
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        362..588
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             589..769
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    816..941
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1434..1667
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1852..1896
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2071..2571
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2490..2508
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2583..2978
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3272..3575
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3576..3779
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3730..3750
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3802..3826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4096..4605
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     protein_bind    complement(4679..4703)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4705..4746
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4788..5376
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4841..4861)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5259..5270)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5448..5681
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5759..5880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5796..5815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5850..5869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5920..6055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6075..6094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6076..6094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6104..6121)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6104..6120)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6113..6135)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6262..6717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6349..6368)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6559..6580
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6743..6847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6848..6916,6917..7708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7066..7085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7879..8467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8368..8387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
       61 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      121 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      181 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      241 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      301 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      361 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      421 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      481 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      541 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      601 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      661 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      721 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      781 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      841 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      901 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      961 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1021 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1081 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1141 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1201 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1261 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1321 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1381 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1441 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1501 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1561 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1621 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1681 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1741 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1801 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1861 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1921 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1981 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2041 cagacccacc tcccaacccc gaggggaccc ttgcgccttt tccaaggcag ccctgggttt
     2101 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     2161 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     2221 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     2281 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     2341 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     2401 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     2461 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     2521 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggggtacca
     2581 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     2641 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     2701 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     2761 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     2821 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     2881 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     2941 ttcgtgaatt gctgccctct ggttatgtgt gggagggcct gcagctgcag tagtaagaat
     3001 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gcgccggctc gagggggccc ggggttatta atagtaatca attacggggt
     3241 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     3301 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3361 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     3421 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     3481 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     3541 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     3601 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     3661 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     3721 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     3781 tctggctaac tgtcgggatc aacaagtttg tacaaaaaag caggcttaat ggccctgtgg
     3841 atgcgcttcc tgcccctgct ggccctgctc ttcctctggg agtcccaccc cacccaggct
     3901 tttgtcaagc agcacctttg tggttcccac ctggtggagg ctctctacct ggtgtgtggg
     3961 gagcgtggct tcttctacac acccatgtcc cgccgtgaag tggaggaccc acaagtggca
     4021 caactggagc tgggtggagg cccgggagca ggtgaccttc agaccttggc actggaggtg
     4081 gcccagcaga agagggtctt cacactcgaa gatttcgttg gggactggcg acagacagcc
     4141 ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc
     4201 ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc
     4261 gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa
     4321 aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgcactat
     4381 ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg acggccgtat
     4441 gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc
     4501 aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt ccgagtaacc
     4561 atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgaagcg tggcattgta
     4621 gatcagtgct gcaccagcat ctgctccctc taccagctgg agaactactg caactagtac
     4681 ccagctttct tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat
     4741 tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg
     4801 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4861 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4921 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4981 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5041 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5101 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5161 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5221 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5281 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5341 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     5401 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5461 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5521 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5581 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5641 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     5701 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     5761 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5821 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5881 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     5941 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6001 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     6061 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     6121 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6181 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6241 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6301 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6361 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6421 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6481 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6541 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6601 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6661 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     6721 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6781 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6841 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6901 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6961 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7021 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7081 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7141 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7201 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7261 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7321 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7381 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7441 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7501 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7561 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7621 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7681 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7741 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7801 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7861 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7921 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7981 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8041 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8101 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8161 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8221 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8281 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8341 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8401 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8461 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8521 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgc
//
