LOCUS       sequence-243418-        4007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part P- Promoter (non-constitutive); Pbad (I13453), arabinose 
            induced; controlled by AraC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-Pbad_P
FEATURES             Location/Qualifiers
     source          1..4007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     119..138
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    240..260
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             288..308
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             328..1035
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             328..1035
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(352..380)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     975..995
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1075..1169
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1242..1333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1334..1402,1403..2194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2198..2237
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2384..2492
                     /note="incP origin of transfer"
     rep_origin      2528..3116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3017..3036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3270..3287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3302..3442
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3368..3387)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3528..3550
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3544..3735)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3915..4001)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagacatt gattatttgc acggcgtcac actttgctat
      121 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
      181 tctactgttt ctccataccg tttttttggg ctagctactt gagacctttc atcgcgacct
      241 actagtagcg gccgctgcag ggagtaactt ctgactgagt tgcatacttg tttaacttta
      301 agaaggagat atacaatggt agcccgtaaa ggagaagaac ttttcactgg agttgtccca
      361 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      421 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      481 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      541 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      601 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      661 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      721 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      781 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      841 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      901 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      961 aagagagatc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1021 gatgaactat acaaaggttc gtaacttctg actgagttgc acgctctagt cttggactcc
     1081 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     1141 gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt
     1201 agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat
     1261 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1321 aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc
     1381 gttttgcctg ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga
     1441 tcaactgggt gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga
     1501 atcttttcgt ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg
     1561 tgcggttctg agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag
     1621 ccagaacgat ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac
     1681 cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct
     1741 gctgacgacc attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca
     1801 tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg
     1861 tgataccacc atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct
     1921 gctgaccctg gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg
     1981 tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg
     2041 cgaacgtggc agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat
     2101 tgtggtgatt tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc
     2161 ggaaattggc gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg
     2221 gagccttttt ttttggacga cccttgtcct tttccgctgc ataaccctgc ttcggggtca
     2281 ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa
     2341 gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag
     2401 taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc
     2461 tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa aagatcaaag
     2521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2641 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3301 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     3361 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     3421 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     3481 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acagtctccg ggagctgcat
     3541 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     3601 agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     3661 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     3721 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     3781 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     3841 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggggc gcgcccagct
     3901 gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     3961 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcct
//
