LOCUS       sequence-243420-        3953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part P- Promoter (non-constitutive); Ptet; induced by 
            tetracycline/analogue; controlled by TetR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-Ptet_P
FEATURES             Location/Qualifiers
     source          1..3953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    133..151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    186..206
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             234..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             274..981
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             274..981
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(298..326)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     921..941
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1021..1115
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1188..1279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1280..1348,1349..2140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2144..2183
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2330..2438
                     /note="incP origin of transfer"
     rep_origin      2474..3062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2963..2982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3216..3233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3248..3388
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3314..3333)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3474..3496
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3490..3681)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3861..3947)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagttttc agcaggacgc actgacctcc ctatcagtga
      121 tagagattga catccctatc agtgatagag atactgagca ctacttgaga cctttcatcg
      181 cgacctacta gtagcggccg ctgcagggag taacttctga ctgagttgca tacttgttta
      241 actttaagaa ggagatatac aatggtagcc cgtaaaggag aagaactttt cactggagtt
      301 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      361 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      421 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
      481 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
      541 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      601 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      661 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      721 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      781 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      841 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
      901 aacgaaaaga gagatcacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
      961 ggcatggatg aactatacaa aggttcgtaa cttctgactg agttgcacgc tctagtcttg
     1021 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     1081 tcggttgccg ccgggcgttt tttattggtg agaatccagg ggtccccaat aattacgatt
     1141 taaattagta gcccgcctaa tgagcgggct tttttttaat tcccctattt gtttattttt
     1201 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1261 atattgaaaa aggaagagta tgagcattca gcattttcgt gtggcgctga ttccgttttt
     1321 tgcggcgttt tgcctgccgg tgtttgcgca tccggaaacc ctggtgaaag tgaaagatgc
     1381 ggaagatcaa ctgggtgcgc gcgtgggcta tattgaactg gatctgaaca gcggcaaaat
     1441 tctggaatct tttcgtccgg aagaacgttt tccgatgatg agcaccttta aagtgctgct
     1501 gtgcggtgcg gttctgagcc gtgtggatgc gggccaggaa caactgggcc gtcgtattca
     1561 ttatagccag aacgatctgg tggaatatag cccggtgacc gaaaaacatc tgaccgatgg
     1621 catgaccgtg cgtgaactgt gcagcgcggc gattaccatg agcgataaca ccgcggcgaa
     1681 cctgctgctg acgaccattg gcggtccgaa agaactgacc gcgtttctgc ataacatggg
     1741 cgatcatgtg acccgtctgg atcgttggga accggaactg aacgaagcga ttccgaacga
     1801 tgaacgtgat accaccatgc cggcagcaat ggcgaccacc ctgcgtaaac tgctgacggg
     1861 tgagctgctg accctggcaa gccgccagca actgattgat tggatggaag cggataaagt
     1921 ggcgggtccg ctgctgcgta gcgcgctgcc ggctggctgg tttattgcgg ataaaagcgg
     1981 tgcgggcgaa cgtggcagcc gtggcattat tgcggcgctg ggcccggatg gtaaaccgag
     2041 ccgtattgtg gtgatttata ccaccggcag ccaggcgacg atggatgaac gtaaccgtca
     2101 gattgcggaa attggcgcga gcctgattaa acattggtaa accgatacaa ttaaaggctc
     2161 cttttggagc cttttttttt ggacgaccct tgtccttttc cgctgcataa ccctgcttcg
     2221 gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt
     2281 gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag
     2341 gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg
     2401 cggtgctcaa cgggaatcct gctctgcgag gctggccgta ggccggcccc gtagaaaaga
     2461 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2521 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2581 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2641 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2701 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2761 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2821 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2881 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2941 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3001 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3061 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3241 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     3301 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     3361 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     3421 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgacag tctccgggag
     3481 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     3541 ctcatcagcg tggtcgtgca gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     3601 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     3661 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     3721 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     3781 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggggcgcgc
     3841 ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     3901 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cct
//
