LOCUS       sequence-243425-        3922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part P- Promoter (non-constitutive); Pcue; metal sensitive; 
            controlled by CueR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-Pcue_P
FEATURES             Location/Qualifiers
     source          1..3922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    155..175
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             203..223
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             243..950
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             243..950
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(267..295)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     890..910
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      990..1084
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1157..1248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1249..1317,1318..2109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2113..2152
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2299..2407
                     /note="incP origin of transfer"
     rep_origin      2443..3031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2932..2951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3185..3202
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3217..3357
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3283..3302)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3443..3465
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3459..3650)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3830..3916)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagaattt cttgaccttc cccttgctgg aaggtttaac
      121 ctttatcaca tacttgagac ctttcatcgc gacctactag tagcggccgc tgcagggagt
      181 aacttctgac tgagttgcat acttgtttaa ctttaagaag gagatataca atggtagccc
      241 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      301 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      361 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      421 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
      481 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
      541 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
      601 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
      661 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
      721 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
      781 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
      841 ccacacaatc tgccctttcg aaagatccca acgaaaagag agatcacatg gtccttcttg
      901 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa ggttcgtaac
      961 ttctgactga gttgcacgct ctagtcttgg actcctgttg atagatccag taatgacctc
     1021 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
     1081 gaatccaggg gtccccaata attacgattt aaattagtag cccgcctaat gagcgggctt
     1141 ttttttaatt cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1201 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagcattcag
     1261 cattttcgtg tggcgctgat tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat
     1321 ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat
     1381 attgaactgg atctgaacag cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt
     1441 ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg
     1501 ggccaggaac aactgggccg tcgtattcat tatagccaga acgatctggt ggaatatagc
     1561 ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg
     1621 attaccatga gcgataacac cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa
     1681 gaactgaccg cgtttctgca taacatgggc gatcatgtga cccgtctgga tcgttgggaa
     1741 ccggaactga acgaagcgat tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg
     1801 gcgaccaccc tgcgtaaact gctgacgggt gagctgctga ccctggcaag ccgccagcaa
     1861 ctgattgatt ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg
     1921 gctggctggt ttattgcgga taaaagcggt gcgggcgaac gtggcagccg tggcattatt
     1981 gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg tgatttatac caccggcagc
     2041 caggcgacga tggatgaacg taaccgtcag attgcggaaa ttggcgcgag cctgattaaa
     2101 cattggtaaa ccgatacaat taaaggctcc ttttggagcc tttttttttg gacgaccctt
     2161 gtccttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc
     2221 atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg
     2281 tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc
     2341 cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg
     2401 ctggccgtag gccggccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2581 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3061 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3121 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3181 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     3241 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     3301 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     3361 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3421 tacagacaag ctgtgacagt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3481 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag
     3541 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     3601 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
     3661 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
     3721 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     3781 ctggcggtat ggatgcggcg ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac
     3841 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     3901 cctttcgttt tatttgatgc ct
//
