LOCUS       sequence-243479-        4008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part T- Terminator;  L3S2P21; synthetic terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 13 to 47)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-L3S2P21_T
FEATURES             Location/Qualifiers
     source          1..4008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..47
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    241..261
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             289..309
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             329..1036
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             329..1036
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(353..381)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     976..996
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1076..1170
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1243..1334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1335..1403,1404..2195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2199..2238
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2385..2493
                     /note="incP origin of transfer"
     rep_origin      2529..3117
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3018..3037
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3271..3288
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3303..3443
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3369..3388)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3529..3551
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3545..3736)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3916..4002)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctgctttcg actcggtacc aaattccaga aaagaggcct
      181 cccgaaaggg gggccttttt tcgttttggt cctgttcgct tgagaccttt catcgcgacc
      241 tactagtagc ggccgctgca gggagtaact tctgactgag ttgcatactt gtttaacttt
      301 aagaaggaga tatacaatgg tagcccgtaa aggagaagaa cttttcactg gagttgtccc
      361 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      421 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      481 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      541 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      601 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      661 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      721 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      781 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      841 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      901 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      961 aaagagagat cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1021 ggatgaacta tacaaaggtt cgtaacttct gactgagttg cacgctctag tcttggactc
     1081 ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt
     1141 tgccgccggg cgttttttat tggtgagaat ccaggggtcc ccaataatta cgatttaaat
     1201 tagtagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta tttttctaaa
     1261 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1321 gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc gctgattccg ttttttgcgg
     1381 cgttttgcct gccggtgttt gcgcatccgg aaaccctggt gaaagtgaaa gatgcggaag
     1441 atcaactggg tgcgcgcgtg ggctatattg aactggatct gaacagcggc aaaattctgg
     1501 aatcttttcg tccggaagaa cgttttccga tgatgagcac ctttaaagtg ctgctgtgcg
     1561 gtgcggttct gagccgtgtg gatgcgggcc aggaacaact gggccgtcgt attcattata
     1621 gccagaacga tctggtggaa tatagcccgg tgaccgaaaa acatctgacc gatggcatga
     1681 ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga taacaccgcg gcgaacctgc
     1741 tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt tctgcataac atgggcgatc
     1801 atgtgacccg tctggatcgt tgggaaccgg aactgaacga agcgattccg aacgatgaac
     1861 gtgataccac catgccggca gcaatggcga ccaccctgcg taaactgctg acgggtgagc
     1921 tgctgaccct ggcaagccgc cagcaactga ttgattggat ggaagcggat aaagtggcgg
     1981 gtccgctgct gcgtagcgcg ctgccggctg gctggtttat tgcggataaa agcggtgcgg
     2041 gcgaacgtgg cagccgtggc attattgcgg cgctgggccc ggatggtaaa ccgagccgta
     2101 ttgtggtgat ttataccacc ggcagccagg cgacgatgga tgaacgtaac cgtcagattg
     2161 cggaaattgg cgcgagcctg attaaacatt ggtaaaccga tacaattaaa ggctcctttt
     2221 ggagcctttt tttttggacg acccttgtcc ttttccgctg cataaccctg cttcggggtc
     2281 attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa
     2341 agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa
     2401 gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg
     2461 ctcaacggga atcctgctct gcgaggctgg ccgtaggccg gccccgtaga aaagatcaaa
     2521 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2581 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2641 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     2701 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2761 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2821 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2881 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2941 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3001 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3061 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3121 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3181 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3241 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3301 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     3361 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     3421 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     3481 gggcttgtct gctcccggca tccgcttaca gacaagctgt gacagtctcc gggagctgca
     3541 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     3601 cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     3661 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     3721 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     3781 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     3841 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcggggg cgcgcccagc
     3901 tgtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     3961 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcct
//
