LOCUS       sequence-243481-        3997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part T- Terminator;  L3S1P11; synthetic terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 13 to 47)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-L3S1P11_T
FEATURES             Location/Qualifiers
     source          1..3997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..47
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    230..250
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             278..298
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             318..1025
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             318..1025
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(342..370)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     965..985
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1065..1159
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1232..1323
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1324..1392,1393..2184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2188..2227
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2374..2482
                     /note="incP origin of transfer"
     rep_origin      2518..3106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3007..3026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3260..3277
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3292..3432
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3358..3377)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3518..3540
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3534..3725)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3905..3991)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctgctttcg agacgaacaa taaggcctcc cttcgggggg
      181 gcctttttta ttgatacaaa atgttcgctt gagacctttc atcgcgacct actagtagcg
      241 gccgctgcag ggagtaactt ctgactgagt tgcatacttg tttaacttta agaaggagat
      301 atacaatggt agcccgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      361 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      421 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      481 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
      541 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      601 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      661 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      721 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      781 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
      841 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      901 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagatc
      961 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     1021 acaaaggttc gtaacttctg actgagttgc acgctctagt cttggactcc tgttgataga
     1081 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc
     1141 gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc
     1201 ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat
     1261 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1321 agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg
     1381 ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt
     1441 gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga atcttttcgt
     1501 ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg
     1561 agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat
     1621 ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa
     1681 ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc
     1741 attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt
     1801 ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc
     1861 atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg
     1921 gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg
     1981 cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc
     2041 agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt
     2101 tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc
     2161 gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt
     2221 ttttggacga cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
     2281 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
     2341 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc
     2401 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
     2461 tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa aagatcaaag gatcttcttg
     2521 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2581 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2641 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2701 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2761 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2821 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2881 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2941 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3001 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3061 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3121 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3181 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3241 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     3301 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     3361 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     3421 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3481 ctcccggcat ccgcttacag acaagctgtg acagtctccg ggagctgcat gtgtcagagg
     3541 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     3601 tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     3661 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     3721 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     3781 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     3841 tgtgagggta aacaactggc ggtatggatg cggcgggggc gcgcccagct gtctagggcg
     3901 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3961 agtctttcga ctgagccttt cgttttattt gatgcct
//
