LOCUS       sequence-243494-        3979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part CT- C-terminus + Terminator; L2U2H09. C-termini STOP + 
            terminator (synthetic).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 13 to 47)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-L2U2H09_CT
FEATURES             Location/Qualifiers
     source          1..3979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..47
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    212..232
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             260..280
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             300..1007
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             300..1007
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(324..352)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     947..967
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1047..1141
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1214..1305
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1306..1374,1375..2166)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2170..2209
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2356..2464
                     /note="incP origin of transfer"
     rep_origin      2500..3088
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2989..3008
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3242..3259
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3274..3414
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3340..3359)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3500..3522
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3516..3707)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3887..3973)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctttcgtga tcgaacggcc ctcgcaaggg ccgttttttt
      181 gtatgttcgc ttgagacctt tcatcgcgac ctactagtag cggccgctgc agggagtaac
      241 ttctgactga gttgcatact tgtttaactt taagaaggag atatacaatg gtagcccgta
      301 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      361 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      421 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      481 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      541 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      601 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      661 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      721 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      781 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      841 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
      901 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga tcacatggtc cttcttgagt
      961 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaaggt tcgtaacttc
     1021 tgactgagtt gcacgctcta gtcttggact cctgttgata gatccagtaa tgacctcaga
     1081 actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa
     1141 tccaggggtc cccaataatt acgatttaaa ttagtagccc gcctaatgag cgggcttttt
     1201 tttaattccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1261 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag cattcagcat
     1321 tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg
     1381 gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt
     1441 gaactggatc tgaacagcgg caaaattctg gaatcttttc gtccggaaga acgttttccg
     1501 atgatgagca cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc
     1561 caggaacaac tgggccgtcg tattcattat agccagaacg atctggtgga atatagcccg
     1621 gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt
     1681 accatgagcg ataacaccgc ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa
     1741 ctgaccgcgt ttctgcataa catgggcgat catgtgaccc gtctggatcg ttgggaaccg
     1801 gaactgaacg aagcgattcc gaacgatgaa cgtgatacca ccatgccggc agcaatggcg
     1861 accaccctgc gtaaactgct gacgggtgag ctgctgaccc tggcaagccg ccagcaactg
     1921 attgattgga tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct
     1981 ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg
     2041 gcgctgggcc cggatggtaa accgagccgt attgtggtga tttataccac cggcagccag
     2101 gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct gattaaacat
     2161 tggtaaaccg atacaattaa aggctccttt tggagccttt ttttttggac gacccttgtc
     2221 cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     2281 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     2341 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     2401 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     2461 gccgtaggcc ggccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2521 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2581 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2641 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2701 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2761 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2821 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2881 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2941 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3001 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3061 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3121 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3181 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3241 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     3301 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     3361 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     3421 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3481 agacaagctg tgacagtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3541 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgcagcga ttcacagatg
     3601 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     3661 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     3721 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     3781 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     3841 gcggtatgga tgcggcgggg gcgcgcccag ctgtctaggg cggcggattt gtcctactca
     3901 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
     3961 ttcgttttat ttgatgcct
//
