LOCUS       sequence-243530-       14711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pmbfA::gRNA2(cexA)::ttrpC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14711)
  AUTHORS   Steiger MG, Rassinger A, Mattanovich D, Sauer M
  TITLE     Engineering of the citrate exporter protein enables high citric acid
            production in Aspergillus niger.
  JOURNAL   Metab Eng. 2019 Mar;52:224-231. doi: 10.1016/j.ymben.2018.12.004. 
            Epub 2018 Dec 13.
  PUBMED    30553933
REFERENCE   2  (bases 1 to 14711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-03"; volume: "52:224-231. doi"; pages: " 
            10.1016/j.ymben.2018.12.004. Epub 2018 Dec 13"
COMMENT     Sequence Label: pMST-711
FEATURES             Location/Qualifiers
     source          1..14711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(62..650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(142..161)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     ncRNA           2243..2279
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        2300..2375
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           2380..2447
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     terminator      2463..3236
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     CDS             4551..8654
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4551..8654
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4551..8654
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8667..8687
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8695..9468
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     regulatory      9624..9633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10217..11242
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10217..11242
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 cgctgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      661 tgatcttttc tacggggtgg agcgcaaaaa accccgcccc tgacagggcg gggttttttc
      721 gcgatcggag gtacagtggc catgaaatcc aatcatttcc ttctggccgc cctcgggcaa
      781 gagatagtgc cgcagaggac tctcacagca tctacatctg cgaccgcaac agccaccaag
      841 cgaggcgcac atgagcttgt cctcctccca tgccaaagtt tggccctctt cgtttctgtg
      901 atgctgaagg aagtcaaact cgtcgatgat aggaccagat ggtttgtcaa gggtcaacgc
      961 tttccatgcc ttctggcacc ggtagtaatg ctcttctgca agggagactt gacgtttcgg
     1021 atccgcgggc cccgggacat gctggaaggg attttctggc tcaataccac gtctgtattt
     1081 gaccctttcc agacagttaa tccgctgcag gagggcgaac tgtagctcct cgttctcctt
     1141 gtagcgcttg atccagtctt tttggatgtt gcacttgctt ggcctatgct tctcatataa
     1201 tcttgccctg tcatagagac gacgtctgag attgtagcgt tcgtctttga tcacccggag
     1261 ccagataggc ctgagtatat ctgacattag atcaaagggt ctgtggatag tctccttcag
     1321 catcagcgac gcatgtgact cgcatgtcgg agagagcttg tgggtggtca tctttgatgg
     1381 cgtcctctgc tttcccttga ttttcgttga ttgtttttcg aaagttaagt ctggaagtca
     1441 agagaatcct tctgccagac attatattta cgtatactga cgtagtagaa acagcgtcag
     1501 gatgaggaca tggtgtgtgc tggaccacgg aatcatagtt catcagtata ttgggttgga
     1561 caaataacgc tgagcatgta tatgtcttta cacactataa aagccagcga acgccaataa
     1621 aatagggcat attgatgtga aaatatgaca ccagttaaaa gcagtgtatt gattttatct
     1681 ctcttcacct cggacctata ctaccgtata caagactcaa cttacttcca gatatagtaa
     1741 tatacaccct atggacgaac cagcacaata attacagcca aacaacacca cccaaatggc
     1801 atattcctaa tcagcactaa gcacaaatac cactgtcatc acagcataat caataagaat
     1861 cccagacaac cgactcactc tgactcacct tacacaaacc cccaagcaaa gcgcagccca
     1921 gaacctcagc caacaatcgg gcaacgtacg gggaaagatt ggccgatcca tgatgtcagc
     1981 agccctaacc caaagcggac tagcgcatac cgcccctctg actccgccat cccagggctc
     2041 gagaagcttc cgtggcgtcg atataaattc agcgggcctt gaacatccct ccttacgaca
     2101 cacctcacgc gatcgatttt gacactcaca caccgccacc ctcacatcct ccacccacac
     2161 cacacccctt aatcaaccca ccatcaccgc tagaacgtct atctcatcac cgacttctca
     2221 tccatcttca aacatgtctc cgctgatgag tccgtgagga cgaaacgagt aagctcgtcc
     2281 ggagaaaata tggcagcgag ttttagagct agaaatagca agttaaaata aggctagtcc
     2341 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttg gccggcatgg tcccagcctc
     2401 ctcgctggcg ccggctgggc aacatgcttc ggcatggcga atgggacagc tttggactgc
     2461 ttgatccact taacgttact gaaatcatca aacagcttga cgaatctgga tataagatcg
     2521 ttggtgtcga tgtcagctcc ggagttgaga caaatggtgt tcaggatctc gataagatac
     2581 gttcatttgt ccaagcagca aagagtgcct tctagtgatt taatagctcc atgtcaacaa
     2641 gaataaaacg cgtttcgggt ttacctcttc cagatacagc tcatctgcaa tgcattaatg
     2701 cattggacct cgcaacccta gtacgccctt caggctccgg cgaagcagaa gaatagctta
     2761 gcagagtcta ttttcatttt cgggagacga gatcaagcag atcaacggtc gtcaagagtc
     2821 ctacgagact gaggaatccg ctcttggctc cacgcgacta tatatttgtc tctaattgta
     2881 ctttgacatg ctcctcttct ttactctgat agcttgacta tgaaaattcc gtcaccagcc
     2941 cctgggttcg caaagataat tgcactgttt cttccttgaa ctctcaagcc tacaggacac
     3001 acattcatcg taggtataaa cctcgaaaat cattcctact aagatgggta tacaatagta
     3061 accatgcatg gttgcctagt gaatgctccg taacacccaa tacgccggcc gaaacttttt
     3121 tacaactctc ctatgagtcg tttacccaga atgcacaggt acacttgttt agaggtaatc
     3181 cttctttcta gaagtcctcg tgtactgtgt aagcgcccac tccacatctc cactcgctcc
     3241 ggggagaacc gaagaggatg ggaaccccga ccatggcctg cggccgcagc tatcagtcct
     3301 ctcagctgac gcccctcacg aaccctcgca ctctcccgaa atgctcttcg gtgtccatgg
     3361 cgcatcatct cagcagcatc ccatgtcaaa ccatggcttt gggcccacgg actctgtggc
     3421 cctgccgcaa catcaccatc accatcgact cccgccccat gcagcgctgc gcgccccagg
     3481 gcgtcatgga gtgaatgtgg aatctcctcc tttgccctcg ggcccgccga gtgtggtggg
     3541 ccagcctgga atgcctgatc cagcccccag gcctcgagga ccaaaactga agtttactcc
     3601 cgaagaggac gctctactgg tggagttgaa ggaaaacaag aacttgacgt ggaagcaaat
     3661 tgcagacttc ttcccgggcc gaacgagcgg taccttgcaa gtccgatact gcaccaagct
     3721 gaaggctaag gatgtagctt ggagtgacga aatggttcga tttgctcctg atgtatttct
     3781 acgcctgtct cacacatgct aatgaaggga ataggtacaa aggctgcagc gggcaatgca
     3841 cgagtacgag aacgatcggt ggcgcatcat tgcagggaag gttggaaatg gcttcacccc
     3901 agctgcttgc cgcgagaaag ccatgcagct ccatgagtaa aagcgttggg gaattttcat
     3961 atttatatct actgtcgcca gattcggccc tgcttggacc ctctgatctc cttactctcc
     4021 atattggttc aaatgtcggg tcaccgatag ggctggtggt gcaggcttgt tgtaggcacg
     4081 ggaggatgat cagcataact ctgagtcact atagggacgg gttgatgtaa ggtattaagt
     4141 gatgtatgat aattcatttt agcccggggg aacatatggc gccggcattt gttcgttcgc
     4201 aatgaaccga cactagcgtc cgctctcgca gtttagcacc ggctgatccc gggctgaacg
     4261 cggccattgc tcggccgggg catgtgttcc ttatctacgg cagaccgcag atgaccactg
     4321 gagcagatta tagaccctaa gccctaagcc ggacacccaa tcgagtaggt ctgcggacca
     4381 ggtcactgcg ggcagccgga gaagctccgc aaccaatcaa tccccggcgc tgactaaggg
     4441 caggcgacca cgggccgaag cggcttcaaa ctcacctcaa cctccaaact ccctcatctc
     4501 caaacgtcct tgccttgtct gccgtcattg caacccaccc accaggacac atggacaaga
     4561 agtactccat tgggctcgat atcggcacaa acagcgtcgg ttgggccgtc attacggacg
     4621 agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa
     4681 agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa gccacgcggc
     4741 tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg
     4801 agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt
     4861 cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg
     4921 acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagcttgtag
     4981 acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat atgatcaaat
     5041 ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac
     5101 tctttatcca actggttcag acttacaatc agcttttcga agagaacccg atcaacgcat
     5161 ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg cggctcgaaa
     5221 acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat cttatcgccc
     5281 tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa gatgccaagc
     5341 ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc cagatcggcg
     5401 accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt ctgctgagtg
     5461 atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt atgatcaagc
     5521 gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga cagcaactgc
     5581 ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc ggatacattg
     5641 acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg gaaaaaatgg
     5701 acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc aaacagcgca
     5761 ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac gctatcctca
     5821 ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt gagaaaatcc
     5881 tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc agattcgcgt
     5941 ggatgactcg caaatcagaa gagactatca ctccctggaa cttcgaggaa gtcgtggata
     6001 agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa aatctgccta
     6061 acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt tataacgagc
     6121 tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg tctggagagc
     6181 agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc gtgaaacagc
     6241 tcaaagagga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc agcggagtgg
     6301 aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc attaaagaca
     6361 aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc ctcaccctta
     6421 cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct catctcttcg
     6481 acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg cggctgtcaa
     6541 gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg gattttctta
     6601 agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac tctctcacct
     6661 ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagcctt cacgagcaca
     6721 tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc gttaaggtcg
     6781 tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt atcgagatgg
     6841 cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg atgaagagga
     6901 ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca gttgaaaaca
     6961 cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg gacatgtacg
     7021 tggatcagga actggacatc aatcggctct ccgactacga cgtggatcat atcgtgcccc
     7081 agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc gataaaaata
     7141 gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa aattattggc
     7201 ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg actaaggctg
     7261 aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag cttgttgaga
     7321 cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac accaagtacg
     7381 atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct aagctcgtct
     7441 cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat taccaccatg
     7501 cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa tatcccaagc
     7561 ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa atgatcgcaa
     7621 agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc aatattatga
     7681 attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga ccacttatcg
     7741 aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc gcgacagtcc
     7801 ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta cagaccggag
     7861 gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc gcacgcaaaa
     7921 aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct tacagtgtac
     7981 tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc aaggaactgc
     8041 tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac tttctcgagg
     8101 cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag tactctctct
     8161 ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg cagaaaggta
     8221 acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc cactatgaaa
     8281 agctcaaagg gtcccccgaa gataatgagc agaagcagct gttcgtggaa caacacaaac
     8341 actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg atcctcgccg
     8401 acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag cccatcaggg
     8461 agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg cctgcagcct
     8521 tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag gaggtcctgg
     8581 acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc gacctctctc
     8641 agctcggtgg agacagcagg gctgacccca agaagaagag gaaggtgtga gcttgatcca
     8701 cttaacgtta ctgaaatcat caaacagctt gacgaatctg gatataagat cgttggtgtc
     8761 gatgtcagct ccggagttga gacaaatggt gttcaggatc tcgataagat acgttcattt
     8821 gtccaagcag caaagagtgc cttctagtga tttaatagct ccatgtcaac aagaataaaa
     8881 cgcgtttcgg gtttacctct tccagataca gctcatctgc aatgcattaa tgcattggac
     8941 ctcgcaaccc tagtacgccc ttcaggctcc ggcgaagcag aagaatagct tagcagagtc
     9001 tattttcatt ttcgggagac gagatcaagc agatcaacgg tcgtcaagag tcctacgaga
     9061 ctgaggaatc cgctcttggc tccacgcgac tatatatttg tctctaattg tactttgaca
     9121 tgctcctctt ctttactctg atagcttgac tatgaaaatt ccgtcaccag cccctgggtt
     9181 cgcaaagata attgcactgt ttcttccttg aactctcaag cctacaggac acacattcat
     9241 cgtaggtata aacctcgaaa atcattccta ctaagatggg tatacaatag taaccatgca
     9301 tggttgccta gtgaatgctc cgtaacaccc aatacgccgg ccgaaacttt tttacaactc
     9361 tcctatgagt cgtttaccca gaatgcacag gtacacttgt ttagaggtaa tccttctttc
     9421 tagaagtcct cgtgtactgt gtaagcgccc actccacatc tccactcgct aattcatgaa
     9481 taacggtgag actagcggcc ggtcccctta tcccagctgt tccacgttgg cctgcccctc
     9541 agttagcgct caactcaatg cccctcactg gcgaggcgag ggcaaggatg gaggggcagc
     9601 atcgcctgag ttggagcaaa gcggccgcca tgggagcagc gaaccaacgg agggatgccg
     9661 tgctttgtcg tggctgctgt ggccaatccg ggcccttggt tggctcacag agcgttgctg
     9721 tgagtccatg agctattatt gctaggtaca gtatagagag aggagagaga gagagagaga
     9781 gagaggggaa aaaaggtgag gttgaagtga gaaaaaaaaa aaaaaaaatc caaccactga
     9841 cggctgccgg ctctgccacc cccctccctc caccccagac cacctgcaca ctcagcgcgc
     9901 agcatcacct aatcttggct cgccttcccg cagctcaggt tgtttttttt ttctctctcc
     9961 ctcgtcgaag ccgcccttgt tcccttattt atttccctct ccatccttgt ctgcctttgg
    10021 tccatctgcc cctttgtctg catctctttt gcacgcatcg ccttatcgtc gtctcttttt
    10081 tcactcacgg gagcttgacg atgacctgac tcgtgagcct cacctgctga tttctctccc
    10141 cccctcccga ccggcttgac ttttgtttct cctccagtac cttatcgcga agccggaaga
    10201 acctcttaac ctctagatga aaaagcctga actcaccgcg acgtctgtcg agaagttcct
    10261 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
    10321 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
    10381 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
    10441 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
    10501 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
    10561 cgcggaggcc atggatgcga tcgctgcggc cgatctcagc cagacgagcg ggttcggccc
    10621 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
    10681 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
    10741 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
    10801 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
    10861 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
    10921 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcacccgga
    10981 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
    11041 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
    11101 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
    11161 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
    11221 tcgtccgagg gcaaaggaat agatgcatgg ctttcgtgac cgggcttcaa acaatgatgt
    11281 gcgatggtgt ggttcccggt tggcggagtc tttgtctact ttggttgtct gtcgcaggtc
    11341 ggtagaccgc aaatgagcaa ctgatggatt gttgccagcg atactataat tcacatggat
    11401 ggtctttgtc gatcagtagc tagtgagaga gagagaacat ctatccacaa tgtcgagtgt
    11461 ctattagaca tactccgaga ataaagtcaa ctgtgtctgt gatctaaaga tcgattcggc
    11521 agtcgagtag cgtataacaa ctccgagtac cagcgaaagc acgtcgtgac aggagcaggg
    11581 ctttgccaac tgcgcaacct tgcttgaatg aggatacacg gggtgcaaca tggctgtact
    11641 gatccatcgc aaccaaaatt tctgtttata gatcaagctg gtagattcca attactccac
    11701 ctcttgcgct tctccatgac atgtaagtgc acgtggaaac catacccaaa ttgcctacag
    11761 ctgcggagca tgagcctatg gcgatcagtc tggtcatgtt aaccagcctg tgctctgacg
    11821 ttaatgcaga atagcttatg ctgcagacgc gttacgtatc ggatccagaa ttcgtgatat
    11881 ctgaattcgt cgacaagctt attttttgta tactgttttg tgatagcacg aagtttttcc
    11941 acggtatctt gttaaaaata tatatttgtg gcgggcttac ctacatcaaa ttaataagag
    12001 actaattata aactaaacac acaagcaagc tactttaggg taaaagttta taaatgcttt
    12061 tgacgtataa acgttgcttg tatttattat tacaattaaa ggtggataga aaacctagag
    12121 actagttaga aactaatctc aggtttgcgt taaactaaat cagagcccga gaggttaaca
    12181 gaacctagaa ggggactaga tatccgggta gggaaacaaa aaaaaaaaac aagacagcca
    12241 catattaggg agactagtta gaagctagtt ccaggactag gaaaataaaa gacaatgata
    12301 ccacagtcta gttgacaact agatagattc tagattgagg ccaaagtctc tgagatccag
    12361 gttagttgca actaatacta gttagtatct agtctcctat aactctgaag ctagaataac
    12421 ttactactat tatcctcacc actgttcagc tgcgcaaacg gagtgattgc aaggtgttca
    12481 gagactagtt attgactagt cagtgactag caataactaa caaggtatta acctaccatg
    12541 tctgccatca ccctgcactt cctcgggctc agcagccttt tcctcctcat tttcatgctc
    12601 attttccttg tttaagactg tgactagtca aagactagtc cagaaccaca aaggagaaat
    12661 gtcttaccac tttcttcatt gcttgtctct tttgcattat ccatgtctgc aactagttag
    12721 agtctagtta gtgactagtc cgacgaggac ttgcttgtct ccggattgtt ggaggaactc
    12781 tccagggcct caagatccac aacagagcct tctagatgac tggtcaataa ctagttggtc
    12841 tttgtctgag tctgacttac gaggttgcat actcgctccc tttgcctcgt caatcgatga
    12901 gaaaaagcgc caaaactcgc aatatggctt tgaaccacac ggtgctgaga ctagttagaa
    12961 tctagtccca aactagcttg gatagcttac ctttgccctt tgcgttgcga caggtcttgc
    13021 agggtatggt tcctttctca ccagctgatt tagctgcctt gctaccctca cggcggatct
    13081 gcataaagag tggctagagg ttataaatta gcactgatcc taggtacggg gctgaatgta
    13141 acttgccttt cctttctcat cgcgcggcaa gacaggcttg ctcaaattcc taccagtcac
    13201 aggggtatgc acggcgtacg gaccacttga actagtcaca gattagttag caactagtct
    13261 gcattgaatg gctgtactta cgggccctcg ccattgtcct gatcatttcc agcttcaccc
    13321 tcgttgctgc aaagtagtta gtgactagtc aaggactagt tgaaatggga gaagaaactc
    13381 acgaattctc gacaccctta gtattgtggt ccttggactt ggtgctgcta tatattagct
    13441 aatacactag ttagactcac agaaacttac gcagctcgct tgcgcttctt ggtaggagtc
    13501 ggggttggga gaacagtgcc ttcaaacaag ccttcatacc atgctacttg actagtcagg
    13561 gactagtcac caagtaatct agataggact tgcctttggc ctccatcagt tccttcatag
    13621 tgggaggtcc attgtgcaat gtaaactcca tgccgtggga gttcttgtcc ttcaagtgct
    13681 tgaccaatat gtttctgttg gcagagggaa cctgtcaact agttaataac tagtcagaaa
    13741 ctagtatagc agtagactca ctgtacgctt gaggcatccc ttcactcggc agtagacttc
    13801 atatggatgg atatcaggca cgccattgtc gtcctgtgga ctagtcagta actaggctta
    13861 aagctagtcg ggtcggctta ctatcttgaa atccggcagc gtaagctccc cgtccttaac
    13921 tgcctcgaga tagtgacagt actctgggga ctttcggaga tcgttatcgc gaatgctcgg
    13981 catactaatc gttgactagt cttggactag tcccgagcaa aaaggattgg aggaggagga
    14041 ggaaggtgag agtgagacaa agagcgaaat aagagcttca aaggctatct ctaagcagta
    14101 tgaaggttaa gtatctagtt cttgactaga tttaaaagag atttcgacta gttatgtacc
    14161 tggagtttgg atataggaat gtgttgtggt aacgaaatgt aagggggagg aaagaaaaag
    14221 tcggtcaaga ggtaactcta agtcggccat tcctttttgg gaggcgctaa ccataaacgg
    14281 catggtcgac ttagagttag ctcagggaat ttagggagtt atctgcgacc accgaggaac
    14341 ggcggaatgc caaagaatcc cgatggagct ctagctggcg gttgacaacc ccaccttttg
    14401 gcgtttctgc ggcgttgcag gcgggactgg atacttcgta gaaccagaaa ggcaaggcag
    14461 aacgcgctca gcaagagtgt tggaagtgat agcatgatgt gccttgttaa ctaggtcaaa
    14521 atctgcagta tgcttgatgt tatccaaagt gtgagagagg aaggtccaaa catacacgat
    14581 tgggagaggg cctaggtata agagtttttg agtagaacgc atgtgagccc agccatctcg
    14641 aggagattaa acacgggccg gcatttgatg gctatgttag taccccaatg gaaagcctga
    14701 gagtccagtg g
//
