LOCUS       sequence-243539-        8901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CDA1-BE4max-NG in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson 
            C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR
  TITLE     Continuous evolution of base editors with expanded target 
            compatibility and improved activity.
  JOURNAL   Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 
            10.1038/s41587-019-0193-0.
  PUBMED    31332326
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 
            10.1038/s41587-019-0193-0."
COMMENT     Sequence Label: pBT371
FEATURES             Location/Qualifiers
     source          1..8901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             718..1338
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="TDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGERR
                     ACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKIL
                     EWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIFI
                     QSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             1435..5535
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5566..5814
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5845..6093
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6136..6156
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6165..6182
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6205..6222)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6211..6435
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6506..6522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6506..6522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6519..6541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6530..6546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6554..6560,6561..6578,6579..6584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6599..6620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6737..6754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6908..7496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6988..7007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7667..8458,8459..8527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8290..8309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8528..8632)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8711..8730)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcagtacc
      721 gacgccgagt acgtgcggat ccacgagaag ctggatatct acacattcaa gaagcagttc
      781 tttaacaaca agaagtccgt gtctcacaga tgctacgtgc tgttcgagct gaagcggaga
      841 ggagagaggc gcgcctgttt ttggggctat gccgtgaaca agccacagtc tggaaccgag
      901 aggggaatcc acgcagagat cttcagcatc aggaaggtgg aggagtacct gcgcgacaac
      961 cccggccagt ttacaatcaa ttggtatagc tcctggagcc cttgcgccga ttgtgccgag
     1021 aagatcctgg agtggtacaa ccaggagctg aggggcaatg gccacaccct gaagatctgg
     1081 gcctgcaagc tgtactatga gaagaacgcc aggaatcaga tcggcctgtg gaacctgcgc
     1141 gacaatggcg tgggcctgaa cgtgatggtg tccgagcact atcagtgctg tcgcaagatc
     1201 tttatccagt ctagccacaa tcagctgaac gagaatcggt ggctggagaa gacactgaag
     1261 agagccgaga agcggagaag cgagctgtcc atcatgatcc aggtgaagat cctgcacacc
     1321 acaaagtctc ccgccgtgtc tggcggatct agcggaggat cctctggcag cgagacacca
     1381 ggaacaagcg agtcagcaac accagagagc agtggcggca gcagcggcgg cagcgacaag
     1441 aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac
     1501 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
     1561 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg
     1621 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
     1681 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
     1741 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
     1801 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg
     1861 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag
     1921 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
     1981 ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc
     2041 agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa
     2101 aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc
     2161 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa
     2221 ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc
     2281 gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc
     2341 gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag
     2401 agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg
     2461 cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt
     2521 gacggcggag ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg
     2581 gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg
     2641 accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg
     2701 cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc
     2761 ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc
     2821 tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac
     2881 aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc
     2941 aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag
     3001 ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag
     3061 cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag
     3121 ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg
     3181 gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac
     3241 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg
     3301 acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc
     3361 gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc
     3421 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg
     3481 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc
     3541 tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac
     3601 attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg
     3661 gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg
     3721 gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg
     3781 atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac
     3841 acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac
     3901 gtggaccagg aactggacat caaccggctg tccgactacg atgtggacca tatcgtgcct
     3961 cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac
     4021 cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg
     4081 cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc
     4141 gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa
     4201 acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac
     4261 gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg
     4321 tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac
     4381 gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag
     4441 ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc
     4501 aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg
     4561 aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc
     4621 gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg
     4681 cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc
     4741 ggcttcagca aagagtctat ccggcccaag aggaacagcg ataagctgat cgccagaaag
     4801 aaggactggg accctaagaa gtacggcggc ttcgtgagcc ccaccgtggc ctattctgtg
     4861 ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg
     4921 ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa
     4981 gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg
     5041 ttcgagctgg aaaacggccg gaagagaatg ctggcctctg cccggttcct gcagaaggga
     5101 aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag
     5161 aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag
     5221 cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc
     5281 gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga
     5341 gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc ccctcgggcc
     5401 ttcaagtact ttgacaccac catcgaccgg aaggtgtacc ggagcaccaa agaggtgctg
     5461 gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct
     5521 cagctgggag gtgacagcgg cgggagcggc gggagcgggg ggagcactaa tctgagcgac
     5581 atcattgaga aggagactgg gaaacagctg gtcattcagg agtccatcct gatgctgcct
     5641 gaggaggtgg aggaagtgat cggcaacaag ccagagtctg acatcctggt gcacaccgcc
     5701 tacgacgagt ccacagatga gaatgtgatg ctgctgacct ctgacgcccc cgagtataag
     5761 ccttgggccc tggtcatcca ggattctaac ggcgagaata agatcaagat gctgagcgga
     5821 ggatccggag gatctggagg cagcaccaac ctgtctgaca tcatcgagaa ggagacaggc
     5881 aagcagctgg tcatccagga gagcatcctg atgctgcccg aagaagtcga agaagtgatc
     5941 ggaaacaagc ctgagagcga tatcctggtc cataccgcct acgacgagag taccgacgaa
     6001 aatgtgatgc tgctgacatc cgacgcccca gagtataagc cctgggctct ggtcatccag
     6061 gattccaacg gagagaacaa aatcaaaatg ctgtctggcg gctcaaaaag aaccgccgac
     6121 ggcagcgaat tcgagcccaa gaagaagagg aaagtctaac cggtcatcat caccatcacc
     6181 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6241 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6301 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6361 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6421 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct
     6481 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6541 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat
     6601 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6661 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6721 ggcgcacttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6781 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6841 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6901 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6961 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7021 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7081 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7141 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7201 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7261 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7321 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7381 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7441 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7501 atcctttgat cttttctacg gggtctgaca ctcagtggaa cgaaaactca cgttaaggga
     7561 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7621 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7681 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7741 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7801 taccgcggga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7861 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7921 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7981 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8041 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8101 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8161 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8221 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8281 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8341 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8401 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8461 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8521 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8581 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8641 cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat cccctagggt
     8701 cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     8761 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     8821 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     8881 tacgggccag atatacgcgt t
//
