LOCUS       sequence-243555-        8763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses evoFERNY-BE4max-NG in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson 
            C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR
  TITLE     Continuous evolution of base editors with expanded target 
            compatibility and improved activity.
  JOURNAL   Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 
            10.1038/s41587-019-0193-0.
  PUBMED    31332326
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 
            10.1038/s41587-019-0193-0."
COMMENT     Sequence Label: pBT374
FEATURES             Location/Qualifiers
     source          1..8763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             314..334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             917..5017
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5048..5296
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5327..5575
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5618..5638
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5647..5664
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5687..5704)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5693..5917
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5988..6004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5988..6004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6001..6023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6012..6028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6036..6042,6043..6060,6061..6066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6081..6102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6219..6236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6390..6978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6470..6489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7149..7940,7941..8009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7772..7791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8010..8114)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8193..8212)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8384..8763
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag ccgacggaag
      301 cgagttcgag tcaccaaaga agaagcggaa agtcagtttt gagaggaact acgacccccg
      361 ggagctgaga aaggagacat acctgctgta tgagatcaag tggggcaagt ccggcaagct
      421 gtggaggcac tggtgccaga acaatcgcac acagcacgcc gaggtgtact tcctggagaa
      481 catctttaat gcccggagat tcaatccatc tacccactgt agcatcacat ggtatctgag
      541 ctggtccccc tgcgccgagt gttctcagaa gatcgtggat ttcctgaagg agcaccctaa
      601 cgtgaatctg gagatctatg tggcccggct gtactatcca gagaacgaga ggaataggca
      661 gggcctgcgg gatctggtga attccggcgt gaccatcaga atcatggacc tgccagatta
      721 caactattgc tggaagacct tcgtgagcga tcagggaggc gacgaggatt actggccagg
      781 acacttcgcc ccttggatca agcagtatag cctgaagctg tctggcggat ctagcggagg
      841 atcctctggc agcgagacac caggaacaag cgagtcagca acaccagaga gcagtggcgg
      901 cagcagcggc ggcagcgaca agaagtacag catcggcctg gccatcggca ccaactctgt
      961 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
     1021 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
     1081 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
     1141 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
     1201 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
     1261 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
     1321 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
     1381 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
     1441 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
     1501 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
     1561 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
     1621 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
     1681 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
     1741 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
     1801 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
     1861 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
     1921 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
     1981 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
     2041 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
     2101 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
     2161 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
     2221 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
     2281 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
     2341 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
     2401 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
     2461 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
     2521 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
     2581 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
     2641 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
     2701 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
     2761 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
     2821 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
     2881 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
     2941 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
     3001 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
     3061 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     3121 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     3181 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
     3241 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
     3301 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
     3361 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
     3421 cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
     3481 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
     3541 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
     3601 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
     3661 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
     3721 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
     3781 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
     3841 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
     3901 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
     3961 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
     4021 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
     4081 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
     4141 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
     4201 aaagaccgag gtgcagacag gcggcttcag caaagagtct atccggccca agaggaacag
     4261 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgtgag
     4321 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
     4381 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
     4441 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
     4501 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
     4561 tgcccggttc ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
     4621 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
     4681 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
     4741 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
     4801 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
     4861 caatctggga gcccctcggg ccttcaagta ctttgacacc accatcgacc ggaaggtgta
     4921 ccggagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
     4981 cgagacacgg atcgacctgt ctcagctggg aggtgacagc ggcgggagcg gcgggagcgg
     5041 ggggagcact aatctgagcg acatcattga gaaggagact gggaaacagc tggtcattca
     5101 ggagtccatc ctgatgctgc ctgaggaggt ggaggaagtg atcggcaaca agccagagtc
     5161 tgacatcctg gtgcacaccg cctacgacga gtccacagat gagaatgtga tgctgctgac
     5221 ctctgacgcc cccgagtata agccttgggc cctggtcatc caggattcta acggcgagaa
     5281 taagatcaag atgctgagcg gaggatccgg aggatctgga ggcagcacca acctgtctga
     5341 catcatcgag aaggagacag gcaagcagct ggtcatccag gagagcatcc tgatgctgcc
     5401 cgaagaagtc gaagaagtga tcggaaacaa gcctgagagc gatatcctgg tccataccgc
     5461 ctacgacgag agtaccgacg aaaatgtgat gctgctgaca tccgacgccc cagagtataa
     5521 gccctgggct ctggtcatcc aggattccaa cggagagaac aaaatcaaaa tgctgtctgg
     5581 cggctcaaaa agaaccgccg acggcagcga attcgagccc aagaagaaga ggaaagtcta
     5641 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
     5701 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5761 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5821 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5881 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5941 tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6001 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6061 tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6121 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6181 gggagaggcg gtttgcgtat tgggcgcact tccgcttcct cgctcactga ctcgctgcgc
     6241 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6301 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6361 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6421 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6481 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6541 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6601 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6661 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6721 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6781 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6841 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6901 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6961 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cactcagtgg
     7021 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7081 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7141 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7201 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7261 tctggcccca gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca
     7321 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7381 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7441 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7501 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7561 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7621 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7681 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7741 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7801 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7861 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7921 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7981 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8041 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8101 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8161 cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta
     8221 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     8281 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     8341 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     8401 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     8461 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     8521 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     8581 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     8641 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     8701 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     8761 atg
//
