LOCUS       sequence-243561-        4831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for making zebrafish apoA-IVb.3  in situ probe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Otis JP, Zeituni EM, Thierer JH, Anderson JL, Brown AC, Boehm ED, 
            Cerchione DM, Ceasrine AM, Avraham-Davidi I, Tempelhof H, Yaniv K, 
            Farber SA
  TITLE     Zebrafish as a model for apolipoprotein biology: comprehensive 
            expression analysis and a role for ApoA-IV in regulating food 
            intake.
  JOURNAL   Dis Model Mech. 2015 Mar;8(3):295-309. doi: 10.1242/dmm.018754. Epub
            2015 Jan 29.
  PUBMED    25633982
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dmm.018754"; journalName: "Dis Model Mech"; date: 
            "2015-03"; volume: "8"; issue: "3"; pages: "295-309"
COMMENT     Sequence Label: PCRII apoA-IVb.3
FEATURES             Location/Qualifiers
     source          1..4831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1060..1079)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1061..1079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1086..1103)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1086..1102)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1095..1117)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1243..1671
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1330..1349)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1540..1561
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             2015..2809
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2069..2088)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2679..2698
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2827..2895,2896..3687)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3045..3064)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3858..4446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4347..4366
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4600..4617
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4734..4755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4770..4775,4776..4793,4794..4800)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4808..4824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(4813..4831,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatttag gtgacactat agaatactca
       61 agctatgcat caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg
      121 gaattcgccc ttacagacta aaagtgaaga ccatgaaggt tcttgtggtg ctcacacttg
      181 ctgtggttac aggctgccag gccaatatat tctatgctga tgagcccaaa ccacagctgg
      241 agcagctgac agatgcattt tggagctatg tttccaaagc aacacaaacc gcagaggaat
      301 ccattaagat gatcagagag tctcaactgg gtcaagaagt caatgaaaga ttgacccaga
      361 gtgctgatat ggccagtgaa tacgctgtcg tcctcaagaa acaggtggat cctctgactg
      421 aagagctgat gaacaaaatc accaaggaga ctgaagtgct gagggagcgt ctgggccagg
      481 acctgatcaa cgtgagagat aaactcgagc cctatgctga caacatgaag agccagatcc
      541 agcagagagt ggaggagctc agggcggcca tggctccgta tgctgactcc ctggattctg
      601 agaccctgaa ggccactctg ctccagaaga gcgaggagct gagaggaaac ctggagcaga
      661 gcgtgaagga gctgcaggtt cagctggggc cctacactgc tgatctcaag gagaaagtag
      721 accagcatct gcaggagttc cagaagaccg tgactcccct gaccgaggat ctgcaggtcc
      781 agattagaga gagagcccag atggtccagc agagtcttac accctacgct gaagatgtga
      841 aggagaagct ggacccctat gcacagaact tgaaggacca gctcgcttct ctgtatgact
      901 ctttcatcaa gagaaattaa atgcattaaa gacttctctt ttttaccaaa ttaactcttc
      961 acaaacttcg aaatttgaca tatttgatcc aagggcgaat tctgcagata tccatcacac
     1021 tggcggccgc tcgagcatgc atctagaggg cccaattcgc cctatagtga gtcgtattac
     1081 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1141 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1201 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
     1261 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     1321 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     1381 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     1441 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     1501 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     1561 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     1621 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     1681 ttcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg
     1741 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
     1801 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
     1861 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
     1921 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag
     1981 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     2041 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     2101 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     2161 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     2221 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     2281 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatccc accttgctcc
     2341 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     2401 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     2461 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     2521 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     2581 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     2641 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     2701 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     2761 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg
     2821 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2881 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2941 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3001 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3061 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3121 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3181 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3241 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3301 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3361 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3421 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3481 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3541 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3601 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3661 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3721 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3781 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3841 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3901 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3961 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4021 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4081 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4141 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4201 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4261 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4321 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4381 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4441 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4501 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4561 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4621 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4681 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     4741 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     4801 tgtggaattg tgagcggata acaatttcac a
//
