LOCUS       sequence-24362-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     T4 Lysozyme Plasmids - Tray 4
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme V131-[A] WT*
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    724..747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(751..757,758..773,774..778))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctacgaaaaa agcccgctca ttaggcgggc tcagatctgc tcatgntttg acagcttatc
       61 atcgataagc ttcggcaata aattcttgat atgttttcat atgtttcctt taaatatttt
      121 aattagtaat tgtctattca agtaattgtg aatatactat cacaattcca agagaaagta
      181 aacagcttta tagattttta tacgcgtccc aagtgccagt tctaaacgtt gtaatgactc
      241 gttttgcgcg attaggtgtt tgattatacc atctactttt agctaagttt gcaactgctg
      301 cttcatccca gcgtttttgt tgaagcatac gtaaagagtt agtaaatcct gccacaccgg
      361 tttctcccat ttggaaaacc atattaatca atgcagcgcg acgaaccgca tcaagagaat
      421 cataaaccgg ttttaattta gcatttctca gaattccgcg aacagcagca tcaacatcct
      481 gattaaagag tttttcagcc tcatcttttg taattacacc attagtatta cgcccaatag
      541 ctttatctaa ttcagattta gcagcattaa gtgatggact ttttgtaagc aaatgaccga
      601 tgccaatagt gtaatagcct tctgtgtctt tatagatttt aagtctaaga ccttcatcta
      661 tacgtaacat ttcaaatata ttcataatac ctcctaagca tcgatggatc ctgtttcctg
      721 tgtgaattgt tatccgctca caattccaca cattatacga gccgatgatn aattgtcaca
      781 gggggatggg gagtagctga tccggttccg ggtgaatgta ncgctccatc cccnatatcg
      841 agcg
//
