LOCUS       sequence-243630-        6119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Human TRIM28 with deleted Linker region 
            between CC and PHD domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Li J, Xi Y, Li W, McCarthy RL, Stratton SA, Zou W, Li W, Dent SY, 
            Jain AK, Barton MC
  TITLE     TRIM28 interacts with EZH2 and SWI/SNF to activate genes that 
            promote mammosphere formation.
  JOURNAL   Oncogene. 2017 May 25;36(21):2991-3001. doi: 10.1038/onc.2016.453. 
            Epub 2017 Jan 9.
  PUBMED    28068325
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2016.453"; journalName: "Oncogene"; date: "2017-05-25- 
            25"; volume: "36"; issue: "21"; pages: "2991-3001"
COMMENT     Sequence Label: HA-TRIM28deltaLinker
FEATURES             Location/Qualifiers
     source          1..6119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        47..1031
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1066..1084
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1066..1083
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1119..1199
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     polyA_signal    3202..3336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3226..3245)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3280..3299
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3449..3469)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3449..3467)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3488..3504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3488..3504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3501..3523)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3512..3528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3536..3542,3543..3560,3561..3566))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3581..3602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3719..3736)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3890..4478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3970..3989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4649..5440,5441..5509))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5272..5291
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5510..5614)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5640..6095)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5777..5798)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5989..6008
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagtcg accgccaatt
       61 caatatggcg tatatggact catgccaatt caatatggtg gatctggacc tgtgccaatt
      121 caatatggcg tatatggact cgtgccaatt caatatggtg gatctggacc ccagccaatt
      181 caatatggcg gacttggcac catgccaatt caatatggcg gacctggcac tgtgccaact
      241 ggggaggggt ctacttggca cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag
      301 tccgccatat tgaattggca tggtgccaat aatggcggcc atattggcta tatgccagga
      361 tcaatatata ggcaatatcc aatatggccc tatgccaata tggctattgg ccaggttcaa
      421 tactatgtat tggccctatg ccatatagta ttccatatat gggttttcct attgacgtag
      481 atagcccctc ccaatgggcg gtcccatata ccatatatgg ggcttcctaa taccgcccat
      541 agccactccc ccattgacgt caatggtctc tatatatggt ctttcctatt gacgtcatat
      601 gggcggtcct attgacgtat atggcgcctc ccccattgac gtcaattacg gtaaatggcc
      661 cgcctggctc aatgcccatt gacgtcaata ggaccaccca ccattgacgt caatgggatg
      721 gctcattgcc cattcatatc cgttctcacg ccccctattg acgtcaatga cggtaaatgg
      781 cccacttggc agtacatcaa tatctattaa tagtaacttg gcaagtacat tactattgga
      841 agtacgccag ggtacattgg cagtactccc attgacgtca atggcggtaa atggcccgcg
      901 atggctgcca agtacatccc cattgacgtc aatggggagg ggcaatgacg caaatgggcg
      961 ttccattgac gtaaatgggc ggtaggcgtg cctaatggga ggtctatata agcaatgctc
     1021 gtttagggaa ccgccattct gcctggggac gtcggagcaa gcttgattta ggtgacacta
     1081 tagaatacaa gctacttgtt ctttttgcag gatccatgta cccatacgat gttccagatt
     1141 acgcttatcc ttatgacgta cctgactatg cataccctta tgatgtacca gactacgctg
     1201 gccggccaat ggcggcctcc gcggcggcag cctcggcagc agcggcctcg gccgcctctg
     1261 gcagcccggg cccgggcgag ggctccgctg gcggcgaaaa gcgctccacc gccccttcgg
     1321 ccgcagcctc ggcctctgcc tcagccgcgg cgtcgtcgcc cgcggggggc ggcgccgagg
     1381 cgctggagct gctggagcac tgcggcgtgt gcagagagcg cctgcgaccc gagagggagc
     1441 cccgcctgct gccctgtttg cactcggcct gtagtgcctg cttagggccc gcggcccccg
     1501 ccgccgccaa cagctcgggg gacggcgggg cggcgggcga cggcaccgtg gtggactgtc
     1561 ccgtgtgcaa gcaacagtgc ttctccaaag acatcgtgga gaattatttc atgcgtgata
     1621 gtggcagcaa ggctgccacc gacgcccagg atgcgaacca gtgctgcact agctgtgagg
     1681 ataatgcccc agccaccagc tactgtgtgg agtgctcgga gcctctgtgt gagacctgtg
     1741 tagaggcgca ccagcgggtg aagtacacca aggaccatac tgtgcgctct actgggccag
     1801 ccaagtctcg ggatggtgaa cgtactgtct attgcaacgt acacaagcat gaaccccttg
     1861 tgctgttttg tgagagctgt gatactctca cctgccgaga ctgccagctc aatgcccaca
     1921 aggaccacca gtaccagttc ttagaggatg cagtgaggaa ccagcgcaag ctcctggcct
     1981 cactggtgaa gcgccttggg gacaaacatg caacattgca gaagagcacc aaggaggttc
     2041 gcagctcaat ccgccaggtg tctgacgtac agaagcgtgt gcaagtggat gtcaagatgg
     2101 ccatcctgca gatcatgaag gagctgaata agcggggccg tgtgctggtc aatgatgccc
     2161 agaaggtgac tgaggggcag caggagcgcc tggagcggca gcactggacc atgaccaaga
     2221 tccagaagca ccaggagcac attctgcgct ttgcctcttg ggctctggag agtgacaaca
     2281 acacagccct tttgctttct aagaagttga tctacttcca gctgcaccgg gccctcaaga
     2341 tgattgtgga tcccgtggag ccacatggcg agatgaagtt tcagtgggac ctcaatgcct
     2401 ggaccaagag tgccgaggcc tttggcaaga ttgtggcaga gcgtcctggc actaactcaa
     2461 caggccctgc acccatggcc cctccaagag ccccagggcc cctgagcaag cagggctctg
     2521 gcagcagcca gcccatgtgc cgtgtctgcc agaagccagg cgatctggtt atgtgcaacc
     2581 agtgtgagtt ttgtttccac ctggactgtc acctgccggc cctgcaggat gtaccagggg
     2641 aggagtggag ctgctcactc tgccatgtgc tccctgacct gaaggaggag gatggcagcc
     2701 tcagcctgga tggtgcagac agcactggcg tggtggccaa gctctcacca gccaaccagc
     2761 ggaaatgtga gcgtgtactg ctggccctat tctgtcacga accctgccgc cccctgcatc
     2821 agctggctac cgactccacc ttctccctgg accagcccgg tggcaccctg gatctgaccc
     2881 tgatccgtgc ccgcctccag gagaagttgt cacctcccta cagctcccca caggagtttg
     2941 cccaggatgt gggccgcatg ttcaagcaat tcaacaagtt aactgaggac aaggcagacg
     3001 tgcagtccat catcggcctg cagcgcttct tcgagacgcg catgaacgag gccttcggtg
     3061 acaccaagtt ctctgctgtg ctggtggagc ccccgccgat gagcctgcct ggtgctggcc
     3121 tgagttccca ggagctgtct ggtggccctg gtgatggccc ctgaggcgcg cctctagaac
     3181 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa
     3241 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3301 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3361 atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg
     3421 cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     3481 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3541 tacgagccgg gagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3601 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3661 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4321 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4561 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4621 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4681 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4741 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4801 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4861 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4921 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4981 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5041 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5101 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5161 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5221 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5281 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5341 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5401 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5461 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5581 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     5641 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     5701 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     5761 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     5821 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     5881 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     5941 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     6001 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     6061 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgca
//
